Abstract: ObjectiveTo investigate the expression of cytochrome P4501A(CYP1A1), thymic stromal lymphopoietin receptor(TSLPR) and CC chemokine receptor 3(CCR3) in children with atopic dermatitis and to analyze their correlation with clinical characteristics. 
MethodsA total of 89 children with atopic dermatitis treated in our hospital were selected as atopic dermatitis group, and 80 healthy children who underwent physical examination in our hospital during the same period were selected as healthy group. Three mL of fasting venous blood was collected from the two groups on the 2nd day after enrollment, and peripheral blood mononuclear cells were obtained under density gradient centrifugation. The expressions of CYP1A1 and TSLPR were detected by real-time quantitative PCR(RT-qPCR), and CCR3 expression in peripheral blood mononuclear cells was measured by fluorescent antibody labeling and flow cytometry. The expressions of CYP1A1, TSLPR and CCR3 in children with atopic dermatitis and their correlation with clinical characteristics were analyzed. 
ResultsCompared with healthy group, the expressions of CYP1A1, TSLPR and CCR3 in atopic dermatitis group increased(P＜0.05). The expressions of CYP1A1, TSLPR and CCR3 were not correlated with age, gender and accompanying symptoms of atopic dermatitis, suggesting no significant difference(P＞0.05). The expressions of CYP1A1, TSLPR and CCR3 were correlated with disease severity, family allergy history, family history of atopic dermatitis, and personal allergic history. Compared with the patients with mild and moderate atopic dermatitis, no family history of atopic dermatitis, no family history of atopic dermatitis and no personal allergy, the expression of CYP1A1, TSLPR and CCR3 in patients with severe atopic dermatitis, family history of allergy, family history of atopic dermatitis, and personal history of allergy were significantly higher(P＜0.05). Pearson correlation analysis showed positive correlation between CYP1A1, TSLPR and CCR3(P＜0.05). ROC curve analysis of the predictive value of CYP1A1, TSLPR and CCR3 in children with atopic dermatitis showed that combined detection of CYP1A1, TSLPR and CCR3 in predicting atopic dermatitis in children was higher than that of CYP1A1, TSLPR and CCR3 alone(P＜0.05). 
ConclusionThe expressions of CYP1A1, TSLPR and CCR3 in children with atopic dermatitis increase, which is related to the severity of the disease, family history of allergy, family history of atopic dermatitis, and personal history of allergy. Therefore, combined detection of the three indicators can be used to predict atopic dermatitis in clinical practice.

Key words: dermatitis, atopic, cytochrome P-450 enzyme system, receptors, chemokine ,