Abstract: ObjectiveTo investigate the effect of liraglutide(LIR) on tumor necrosis factor(TNF)-α-induced vascular smooth muscle cell inflammation and its possible molecular mechanism. 
MethodsThe experiment was divided into three groups: blank control group(NC group), TNF-α group(10 μg/L) and TNF-α +LIR group(10 μg/L TNF-α + 100 nmol/L LIR). IL-1β and IL-6 in cell culture medium were detected by enzyme linked-immunosorbent assay(ELISA). The mRNA expressions of ICAM-1, MCP-1 and IκB-α and circ-sirt1 were detected by real-time quantitative polymerase chain reaction detecting system（qPCR）. The protein expressions of ICAM-1, MCP-1 and IκB-α were detected by Western blot. The protein distribution of NF-κB p65 was detected by immunofluorescence. 
ResultsCompared with NC group, the levels of IL-1β and IL-6 in TNF-α group were significantly increased, and the mRNA and protein expressions of ICAM-1 and MCP-1 were significantly increased. The mRNA and protein expression of IκB-α was significantly decreased, and the expression of circ-sirt1 was significantly decreased. NF-κB p65 protein was mainly localized in the nucleus. Compared with TNF-α group, the levels of IL-1β and IL-6 and the protein expressions of ICAM-1 and MCP-1 were significantly decreased, while the protein expression of IκB-α was significantly increased in TNF-α+LIR group. Meanwhile，the expression of circ-sirt1 was significantly increased and nuclear localization of NF-κB p65 protein was decreased in TNF-α+LIR group. 
ConclusionLilarutide can inhibit the inflammatory response of vascular smooth muscle cells, possibly by inhibiting the release of inflammatory mediators and the activation of circ-sirt1/NF-κB signaling pathway.

Key words: liraglutide, myocytes, smooth muscle, NF-κB ,