河北医科大学学报

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高浓度富氢生理盐水对脂多糖诱导大鼠急性肺损伤的作用

  

  1. 1.河北科大学第二医院呼吸与危重症医学一科，河北 石家庄 050000；2.河北医科大学法医学院，
    河北省法医学重点实验室，河北省法医分子鉴定协同创新中心，河北 石家庄 050017
  • 出版日期:2019-12-25 发布日期:2019-12-26
  • 作者简介:徐海博（1983-），男，河北安国人，河北科大学第二医院主治医师，医学硕士，从事呼吸系统疾病及危重症诊治研究。
  • 基金资助:
    河北省卫健委青年科技课题（20180287）

Effects of high concentration hydrogen-rich saline on lipopolysaccharide-induced acute lung injury in rats#br#

  1. 1.The First Department of Respiratory and Critical Care Medicine, the Second Hospital of Hebei Medical
    University， Shijiazhuang 050000, China; 2.College of Forensic Medicine, Hebei Medical University,
    Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic
    Medical Molecular Identification Hebei Province, Shijiazhuang 050017, China
  • Online:2019-12-25 Published:2019-12-26

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摘要： ［摘要］
  目的  探讨高浓度富氢生理盐水对腹腔注射脂多糖诱导急性肺损伤的影响。
  方法  选取60只清洁级SD雄性大鼠，随机分为空白组、高浓度富氢生理盐水组、脂多糖组、脂多糖+高浓度富氢生理盐水组各15只。脂多糖组、脂多糖+富氢生理盐水组腹腔内注射脂多糖10 mg/kg制备大鼠脓毒症模型。富氢生理盐水组、脂多糖+富氢生理盐水组治疗组在术后即刻、3 h、6 h、12 h、18 h，以5 mL/kg高浓度富氢生理盐水腹腔注射。空白组、脂多糖组给予等量生理盐水腹腔注射。取支气管肺泡灌洗液检测中性粒细胞计数，取肺组织检测丙二醛(malondialdehyde，MDA)含量及超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活性，Western blot检测肺组织中核因子E-2-相关因子2（nuclear factor erythroid-2-related factor-2，Nrf2）和血红素加氧酶1（hemeoxygenase-1，HO-1）的表达，比较组间差异。
  结果  与脂多糖组比较，脂多糖+富氢生理盐水组大鼠7 d平均生存时间延长（P＜0.05）；与空白组比较，脂多糖组中性粒细胞计数、MDA含量均明显升高，SOD、CAT、GSH-Px活性均下降（P＜0.05），肺组织中Nrf2和HO-1的蛋白表达上升（P＜0.05）；与脂多糖组比较，脂多糖+富氢生理盐水组中性粒细胞计数、MDA含量均减少，SOD、CAT、GSH-Px活性以及Nrf2和HO-1的蛋白表达均明显增加（P＜0.05）。
  结论  高浓度富氢生理盐水可延长脂多糖所致脓毒症大鼠平均生存时间，减少肺部炎症细胞、氧化应激，可能通过Nrf2/HO-1通路控制急性肺损伤。

关键词: 脓毒症, 急性肺损伤, 脂多糖类, 大鼠

Abstract: ［Abstract］ Objective〖HTSS〗To investigate the effect of high concentration of hydrogen-rich saline on acute lung injury induced by intraperitoneal injection of lipopolysaccharide(LPS).
  〖WTHZ〗Methods〖HTSS〗Sixty male SD rats of clean grade were randomly divided into blank group hydrogen-rich saline group, LPS group and LPS + hydrogen-rich saline group, with 15 rats in each group. LPS group and LPS + hydrogen-rich saline group were injected intraperitoneally with LPS 10 mg/kg to establish sepsis model in rats. hydrogen-rich salin group and LPS + hydrogen-rich saline group were intraperitoneally injected with 5 mL/kg high concentration high concentration hydrogen-rich saline immediately, 3 h, 6 h, 12 h and 18 h after operation. Blank group and LPS 〖JP2〗group were given intraperitoneal injection of normal saline. Bronchoalveolar lavage fluid was used to detect neutrophil count, malondialdehyde(MDA)content, superoxide dismutase(SOD), myeloperoxidase〖JP〗(MPO), glutathione peroxidase(GSH-Px) activity in lung tissue, and Western blot was used to detect the expression of nuclear factor erythroid-2-related factor-2(Nrf2) and hemeoxygenase-1(HO-1)in lung tissue.
  〖WTHZ〗Results〖HTSS〗Compared with LPS group, the mean survival time of rats in LPS + hydrogen-rich salin group was prolonged in 7 days(P＜0.05). Compared with blank group, the number of neutrophils and the content of MDA in LPS group were significantly increased, the activities of SOD, MPO and GSH-Px were decreased(P＜0.05), and the expressions of Nrf2 and HO-1 in lung tissue were increased(P＜0.05). Compared with LPS group, the expression of Nrf2 and HO-1 in lung tissue was increased(P＜0.05). In hydrogen-rich salin group, neutrophil count, MDA content was decreased, SOD, MPO, GSH-Px activity, Nrf2 and HO-1 expression were increased significantly(P＜0.05).
  〖WTHZ〗Conclusion〖HTSS〗High concentration hydrogen-rich saline can prolong the mean survival time of LPS-induced sepsis rats, reduce inflammatory cells and oxidative stress in the lung, and may control acute lung injury through Nrf2/HO-1 pathway.

Key words: sepsis, acute lung injury, lipopolysaccharides, rats

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