河北医科大学学报

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PLK1基因与胃癌AZ521细胞肿瘤生物学行为的研究

  

  1. 1.河北医科大学第四医院胸外科，河北 石家庄 050011；2.河北医科大学基础医学院2017级临床医学四大班，
    河北 石家庄 050017；3.河北医科大学期刊社，河北 石家庄 050017
  • 出版日期:2019-12-25 发布日期:2019-12-26
  • 作者简介:张耀中（1995-），男，河北邯郸人，河北医科大学第四医院医学硕士研究生，从事胸外科疾病诊治研究。
  • 基金资助:
    河北省重点研发计划项目（18397771D）；河北省医学科学研究课题计划（20190409）；河北省医学科学研究重点课题计划（20180528）

Research on the biological behavior of PLK1 gene and gastric carcinoma AZ521 cells#br#

  1. 1.Department of Thoracic Surgery， the Fourth Hospital of Hebei Medical University， Shijiazhuang
    050011, China; 2.2017 Class 4 of Clinical Medicine, the School of Basic Medical Sciences,
    Hebei Medical University， Shijiazhuang 050017, China; 3.Periodical Press,
    Hebei Medical University， Shijiazhuang 050017, China
  • Online:2019-12-25 Published:2019-12-26

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摘要： ［摘要］
  目的  观察抑制PLK1基因表达后对胃癌AZ521细胞生长增殖和侵袭转移的影响，探讨PLK1基因作为胃癌基因治疗靶点的可行性及相关机制。
  方法  应用siRNA干扰技术抑制PLK1基因表达，采用实时荧光定量PCR法检测胃癌AZ521细胞PLK1 mRNA表达水平；采用Western blot法检测AZ521细胞中PLK1、MMP-9、E-cadherin、Vimentin蛋白表达；应用流式细胞仪检测AZ521细胞周期分布的变化；采用Transwell实验检测AZ521细胞侵袭能力的影响。
  结果  siRNA组PLK1 mRNA表达水平明显低于control组（P＜0.05）。siRNA组PLK1、Vimentin、MMP-9蛋白表达明显低于control组，E-cadherin蛋白表达明显高于control组（P＜0.05）。siRNA组G2/M期细胞细胞表达量明显高于control组，S期细胞表达量明显低于control组（P＜0.05）；2组G0/G1期细胞表达量差异无统计学意义（P＞0.05）。siRNA组AZ521细胞穿膜细胞数明显少于control组（P＜0.05）。
  结论  PLK1 siRNA可以阻断PLK1的表达和功能，PLK1在胃癌的生长和侵袭转移中发挥了重要作用。

关键词: 胃肿瘤, PLK1基因, 细胞周期

Abstract: ［Abstract］Objective〖HTSS〗To observe the effect of inhibiting the expression of PLK1 gene on invasion and metastasis of gastric cancer AZ521 cells, and to explore the feasibility and related mechanism of PLK1 gene for gastric cancer therapy.
  〖WTHZ〗Methods〖HTSS〗siRNA interference technology was used to inhibit PLK1 gene expression. The expression of PLK1 mRNA in AZ521 cells was detected by real-time fluorescent quantitative PCR. PLK1, MMP-9, E-cadherin and Vimentin protein expression in AZ521 cells were detected by Western blot. The cell cycle distribution of AZ521 was detected by flow cytometry. Transwell the experiment was used to examine the effect of AZ521 cell invasion ability.
  〖WTHZ〗Results〖HTSS〗The expression level of PLK1 mRNA in siRNA group was significantly lower than that in control group(P＜0.05). The expression of MMP-9 and Vimentin protein was down-regulated, E-cadherin protein expression was significantly up-regulated（P＜0.05）. The G2/M phase cells in the siRNA group were significantly higher than the control group. The S phase cells were significantly lower than the control group(P＜0.05) . There was no significant difference in the G0/G1 phase cells between two groups(P＞0.05). Compared with the control group, The number of transmembrane cells in AZ521 cells in siRNA group was significantly decreased(P＜0.05).
  〖WTHZ〗Conclusion〖HTSS〗PLK1 siRNA can block the expression and function of PLK1, and PLK1 plays an important role in the growth, invasion and metastasis of gastric cancer．

Key words: stomach neoplasms； PLK1 gene, cell cycle

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