河北医科大学学报 ›› 2020, Vol. 41 ›› Issue (11): 1245-1250.doi: 10.3969/j.issn.1007-3205.2020.11.002

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双氢青蒿素对人胃癌细胞BGC-823细胞增殖、凋亡的影响及机制研究

  

  1. 1.河北医科大学教学实验中心，河北 石家庄 050017；2.河北医科大学电镜实验中心，
    河北 石家庄 050017;3.河北医科大学细胞生物教研室，河北 石家庄 050017
  • 出版日期:2020-11-25 发布日期:2020-11-30
  • 作者简介:徐彦楠（1984-），女，河北鹿泉人，河北医科大学实验师，医学硕士，从事细胞凋亡研究。
  • 基金资助:
    河北医科大学临床学院大学生创新实验计划项目（202013415011）；河北医科大学大学生创新实验（USIP2020239）;河北医科大学教育科学研究立项课题（2018YB-55）

Effect of dihydroartemisinin on proliferation and apoptosis of human gastric cancer cell line BGC-823 and its mechanisms

  1. 1.Department of Teaching and Experiment Center, Hebei Medical University, Shijiazhuang 050017， China; 
    2.Department of Electron Microscopy Center, Hebei Medical University,Shijiazhuang 050017， China; 
    3.Department of Cell Biology, Hebei Medical University, Shijiazhuang 050017， China
  • Online:2020-11-25 Published:2020-11-30

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摘要： 研究双氢青蒿素（dihydroartemisinin,DHA）对人胃癌细胞BGC-823细胞增殖、凋亡的影响及机制。
方法  采用噻唑蓝（methylthiazoletetrazolium,MTT）法检测DHA对人胃癌细胞BGC-823增殖的抑制作用；用流式细胞技术、荧光显微镜、透射电子显微镜检测经DHA处理后BGC-823细胞的凋亡率及形态特征的变化；Western印迹方法检测凋亡相关基因Bax、Caspase-3、Caspase-8表达的变化。
结果  MTT分析表明，DHA作用可明显抑制BGC-823细胞的增殖，抑制率随药物浓度的增加而增大，且随着时间的延长而增大，具有显著的剂量和时间依赖性（P＜0.01），半数抑制浓度(IC50值)为3.4 μmol/L。流式细胞术检测结果显示，随着DHA药物浓度的增加，凋亡峰愈加明显，并呈现出剂量依赖性（P＜0.01）。形态学的观察结果：BGC-823细胞在DHA作用下出现典型的凋亡形态。Western印迹显示：Bax、Caspase-3、Caspase-8蛋白表达随着DHA给药浓度的增加而增高。
结论  DHA对BGC-823细胞增殖有抑制作用；DHA通过上调Bax、Caspase-3、Caspase-8表达促进BGC-823细胞凋亡。


关键词: 胃肿瘤, 双氢青蒿素, 细胞增殖, 细胞凋亡

Abstract: Objective  To investigate the effect of dihydroartemisinin on the growth and apoptosis of human gastric cancer cell line BGC-823. 
Methods  Methylthiazoletetrazolium（MTT） assay was used to determine the inhibitory rate of dihydroartemisinin with different concentrations on the proliferation of BGC-823 cells. The apoptosis rate and morphological changes of BGC-823 cells treated with dihydroartemisinin were detected by flow cytometry(FCM), fluorescence microscope, transmission electron microscope. Western-blot were used to detect the changes of Bax,Caspase-3,Caspase-8 protein levels in BGC-823 cells. 
Results  MTT assay showed that the proliferation of BGC-823 cells was markedly inhibited after treatment with dihydroartemisinin, and the inhibition rate increases with the increase of drug concentration, and with the extension of time. It has a significant dose and time dependence(P＜0.01) and IC50 was 3.4 μmol/L. The results of flow cytometry showed that with the increase of drug concentration, the apoptotic peak became more obvious and showed a dose-dependent(P＜0.01). Typical apoptotic morphology were observed in BGC-823 cells after induced by dihydroartemisinin. Western blot showed that the expressions of Bax, Caspase-3 and Caspase-8 proteins increased with the increase of dihydroartemisinin concentration. 
Conclusion  The result suggested that dihydroartemisinin could inhibit the proliferation of BGC-823 cells. Dihydroartemisinin promotes the apoptosis of BGC-823 cells by up-regulating the expression of Bax, Caspase-3 and Caspase-8.


Key words:  , stomach neoplasms, dihydroartemisinin, cell proliferation, apoptosis

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