关键词: 心肌再灌注损伤, sigma-1受体激动剂, PRE-084

Abstract:

Objective  Sigma-1 receptor is a non-opioid receptor, and its agonist has a positive protective effect on the heart. The purpose of this study was to explore application effect of Sigma-1 receptor on myocardial ischemia-reperfusion(IR) injury.

Methods  A total of 24 SD rats included in this study were randomly divided into three groups according to the numerical randomization method, including the IR group(n=8), the Sham operation group(Sham group, n=8) and the PRE-084 group(n=8). The IR and Sham groups received normal saline at 1 h before operation. In the meantime, according to relevant clinical experiment indicators, the PRE-

084 group received the same amount of 1 mg/kg PRE-084, followed by ligation of the anterior descending artery for 30 min and reperfusion for 24 h. The effects of PRE-084 on the area of myocardial infarction, cardiac function, CK-MB, SOD, and MDA were evaluated.

Results  The values of CK-MB and MDA in I/R group and PRE-084 group were higher than those in Sham group, while the values of SOD were lower than those in Sham group. The values of CK-MB and MDA in PRE-084 group were lower than those in I/R group, while the values of SOD were higher than those in I/R group(P＜0.05). At 24 h after reperfusion, compared with Sham group, the +dp/dtmax, -dp/dtmax and LVSP in PRE-084 group and I/R group decreased significantly, and increased significantly in PRE-084 group compared with I/R group(P＜0.05). Compared with Sham group, LVEDP in PRE-084 group and I/R group increased(P＜0.05), and there was no significant difference between I/R group and PRE-084 group(P＞0.05).

Conclusion  PRE-084 pretreatment can reduce the area of myocardial infarction in rats with IR injury by reducing oxygen free radical damage.

Key words: myocardial reperfusion injury, sigma-1 receptor； PRE-084