河北医科大学学报 ›› 2022, Vol. 43 ›› Issue (11): 1261-1266,1276.doi: 10.3969/j.issn.1007-3205.2022.11.005

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miR-21、Caveolin-1、Gal-9在病毒性脑炎患者的表达及相关性研究

  

  1. 1.河北省邯郸市第一医院神经内二科，河北 邯郸 056002；2.河北省邯郸市中心医院神经外三科，河北 邯郸  056001；3.河北省邯郸市第一医院医务科，河北 邯郸 056002

  • 出版日期:2022-11-25 发布日期:2022-12-28
  • 作者简介:王莎（1982-），女，河北武安人，河北省邯郸市第一医院副主任医师，医学学士，从事神经内科疾病诊治研究。
  • 基金资助:
    河北省医学科学研究课题计划（20200439）

Expression and correlation of miR-21, Caveolin-1 and Gal-9 in patients with viral meningitis

  1. 1.The Second Department of Neurology, the First Hospital of Handan City, Hebei Province, Handan 
    056002, China; 2.The Third Department of Neurology, the Central Hospital of Handan City, 
    Hebei Province, Handan 056001, China; 3.Department of Medical Administration, 
    the First Hospital of Handan City, Hebei Province, Handan 056002, China
  • Online:2022-11-25 Published:2022-12-28

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摘要： 目的 探讨病毒性脑炎（viral meningitis，VM）患者脑脊液微小核糖核酸21（micro ribonucleic acid-21，miR-21）表达、陷窝蛋白1（Caveolin-1）、半乳糖凝集素9（galectin-9，Gal-9）水平特点，分析miR-21、Caveolin-1、Gal-9与VM复发的关系。
方法 选择VM患者（VM组）132例和经检查证实无神经系统损伤的患者（对照组）136例。根据病情将VM患者分为重症组（58例）和轻症组（74例），所有患者出院后随访3个月，根据是否VM复发分为复发组（26例）和无复发组（106例）。治疗前后检测血清miR-21表达、Caveolin-1、Gal-9以及炎性因子水平，分析miR-21、Caveolin-1、Gal-9与VM病情、炎性因子以及复发的关系，受试者工作特征曲线（receiver-operating characteristic curve， ROC）分析miR-21、Caveolin-1、Gal-9预测VM复发的价值。
结果 VM组miR-21表达、Caveolin-1、Gal-9、肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）、白细胞介素6（interleukin-6，IL-6）水平高于对照组（P＜0.05）。重症组VM患者脑脊液miR-21表达、Caveolin-1、Gal-9、TNF-α、IL-6水平高于轻症组（P＜0.05）。miR-21、 Caveolin-1、Gal-9水平均与TNF-α、IL-6呈正相关（P＜0.05）。治疗前和治疗前后，复发组脑脊液miR-21表达、Caveolin-1、Gal-9、TNF-α、IL-6水平均高于无复发组，复发组和无复发组患者治疗后脑脊液miR-21表达、Caveolin-1、Gal-9、TNF-α、IL-6水平低于治疗前（P＜0.05）。治疗后高TNF-α、miR-21、 Caveolin-1、Gal-9是VM复发的危险因素（P＜0.05）。联合检测治疗后miR-21、Caveolin-1、Gal-9预测VM复发的AUC为0.858，大于单项指标预测（P＜0.05）。
结论 VM患者脑脊液中miR-21表达、Caveolin-1、Gal-9水平明显增加，治疗后高表达miR-21、高水平Caveolin-1、Gal-9与VM病情严重程度以及复发密切相关。


关键词: 脑炎, 病毒性, miR-21, 陷窝蛋白1, 半乳糖凝集素9

Abstract: Objective To investigate the characteristics of the expression level of micro ribonucleic acid-21(miR-21), Caveolin-1 and galectin-9(Gal-9) in cerebrospinal fluid of patients with viral encephalitis(VM), and to analyze the relationship between miR-21, Caveolin-1, Gal-9 and VM recurrence. 
Methods A total of 132 patients with VM(VM group) and 136 patients without neurological injury confirmed by examination(control group) were selected. VM patients were divided into severe group(n=58) and mild group(n=74) according to their condition. All patients were followed up for 3 months after discharge and divided into recurrence group(n=26) and non-recurrence group(n=106) according to recurrence of VM. The levels of serum miR-21, Caveolin-1, Gal-9 and inflammatory factors were detected before and after treatment, and the relationship between miR-21, Caveolin-1, Gal-9 and VM severity, inflammatory factors and recurrence was analyzed. Receiver operating characteristic(ROC) curve was used to analyze the value of miR-21, Caveolin-1 and Gal-9 in predicting VM recurrence. 
Results The expression of miR-21 and the levels of Caveolin-1, Gal-9, tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in VM group were higher than those in control group(P＜0.05). The expression of miR-21 and the levels of Caveolin-1, Gal-9, TNF-α and IL-6 in cerebrospinal fluid of VM patients in severe group were higher than those in mild group(P＜0.05).The levels of miR-21, Caveolin-1 and Gal-9 were positively correlated with TNF-α and IL-6(P＜0.05). Before and after treatment, the expression of miR-21 and the levels of Caveolin-1, Gsl-9, TNF-α and IL-6 in cerebrospinal fluid of the recurrence group were higher than those of the non-recurrence group. After treatment, the expression of miR-21 and the levels of Caveolin-1, Gal-9, TNF-α and IL-6 in cerebrospinal fluid of patients in recurrence group and non-recurrence group were lower than those before treatment(P＜0.05). After treatment, high levels of TNF-α, miR-21, Caveolin-1 and Gal-9 were risk factors for VM recurrence(P＜0.05). The area under the ROC curve(AUC) of combined detection of miR-21, Caveolin-1 and Gal-9 in predicting VM recurrence was 0.858, which was higher than that of single index(P＜0.05). 
Conclusion The expression levels of miR-21, Caveolin-1 and Gal-9 in the cerebrospinal fluid of VM patients are significantly increased, and the high expression of miR-21, Caveolin-1 and Gal-9 is closely related to the severity and recurrence of VM after treatment.


Key words: encephalitis, viral, micro ribonucleic acid-21, caveolin-1, galectin-9

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