关键词: 子宫腺肌病, miR-103a-3p, miR-101-3p, 可溶性细胞间黏附分子

Abstract: Objective To investigate the expression and clinical significance of miR-103a-3p, soluble intercellular adhesion molecule-1 (sICAM-1), and miR-101-3p before and after treatment of uterine adenomyosis. 
Methods In total,125 patients with uterine adenomyosis in the Third Hospital of Chengde City were selected as the research group, and another 87 healthy physical examinees with normal menstruation and no dysmenorrhea during the same period were selected as the control group. The miR-103a-3p, sICAM-1 and miR-101-3p of the two groups were calculated, and Spearman correlation analysis was performed to analyze the correlation of each indicator with the intensity of dysmenorrhea and efficacy. The receiver operating characteristic (ROC) curve and the area under the ROC curve (AUC) were drawn to analyze the predictive value of each indiator in the efficacy of uterine adenomyosis. 
Results Before treatment, the levels of miR-103a-3p and miR-101-3p in the research group were higher than those in the control group, while the levels of sICAM-1 were lower than those in the control group (P＜0.05). After treatment, the levels of miR-103a-3p and miR-101-3p in the research group were lower than those before treatment, while the levels of sICAM-1 were higher than those before treatment (P＜0.05). Before treatment and at 6 months after treatment, the levels of miR-103a-3p and miR-101-3p in severe patients in the research group were higher than those in mild and moderate patients, while the levels of sICAM-1 were lower than those in mild and moderate patients; the levels of miR-103a-3p and miR-101-3p in moderate patients were higher than those in mild patients, while the levels of sICAM-1 were lower than those in mild patients (P＜0.05). After treatment, the levels of miR-103a-3p and miR-101-3p in both groups were lower than those before treatment, whilethe levels of sICAM-1 were higher than those before treatment (P＜0.05). The levels of miR-103a-3p and miR-101-3p in the effective patients in the research group before treatment and at 6 months after treatment were lower than those in the ineffective patients, while the levels of sICAM-1 were higher than those in the ineffective patients (P＜0.05). MiR-103a-3p and miR-101-3p were positively correlated with the intensity of dysmenorrhea and negatively correlated with the efficacy (P＜0.05); SICAM-1 was negatively correlated with the intensity of dysmenorrhea and positively correlated with the efficacy (P＜0.05). The AUC of the combination of sICAM-1 and miR-101-3p difference in predicting the efficacy of uterine adenomyosis was 0.909 (95%CI: 0.844-0.953), the sensitivity was 84.00%, and the specificity was 81.00%, which were significantly better than the prediction of each indicator alone. 
Conclusion Abnormal expression of miR-103a-3p, sICAM-1, and miR-101-3p in patients with uterine adenomyosis is associated with intensity of dysmenorrhea, and therapeutic effect of uterine arterial embolization(UAE), and can be used as an objective indicator for therapeutic effect evaluation of UAE. 

Key words: adenomyosis, miR-103a-3p, miR-101-3p, sICAM-1