河北医科大学学报

• 论著 • 上一篇    下一篇

库存血加热对于血浆中IL8的影响#br#

  

  1. 1.河北医科大学第四医院输血科，河北 石家庄 050011；2.河北医科大学第四医院干部保健处，河北 石家庄 050011
  • 出版日期:2017-04-25 发布日期:2017-04-25
  • 作者简介:孙楠（1978-），男，河北保定人，河北医科大学第四医院主管技师，医学硕士，从事临床输血研究。

Effect of heating on blood plasma IL8#br#

  1. 1.Department of Blood Transfusion, the Fourth Hospital of Hebei Medical University, Shjiazhuang
    050011, China; 2.Department of Cadre Health Service, the Fourth Hospital of Hebei Medical
    University, Shjiazhuang 050011, China）
  • Online:2017-04-25 Published:2017-04-25

HTML

PDF (PC)

151

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨温浴不同温度和时间对血浆中白细胞介素8（interleukin8，IL8）的影响，为库存红细胞安全、有效的使用提供理论依据。
〖HTH〗方法〖HTSS〗〖KG*2〗随机抽取河北省血液中心采集的无偿献血、经检测各项指标均合格的血液标本50份，每份均分成8段（A、B、C、D、E、F、G、H组）分别行4 ℃保存，37 ℃加热10、20、30、60、120、180 min和40 ℃加热30 min处理，并进行后续实验。采用双抗体夹心酶联免疫吸附测定法检测血浆中IL8浓度。
〖HTH〗结果〖HTSS〗〖KG*2〗加热能明显上调IL8水平，并且有时间依赖性，随加热时间延长IL8水平逐渐增高，B、C组血浆IL8浓度与A组相比差异无统计学意义（P＞005），而D、E、F、G、H组血浆IL8浓度与A组相比显著升高，差异有统计学意义（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗库存红细胞37 ℃温浴30 min以内对血浆IL8浓度无明显影响，之后随着时间延长逐渐升高。

关键词: 输血, 红细胞免疫功能, 白细胞介素8

Abstract: 〗［Abstract］ Objective〖HTSS〗〓To study the effects of storing suspending erythrocytes after water bath on interleukin8（IL8） in plasma, and further more to confirm the effect of water bath at various times and different degrees on IL8 in plasma. Our study will provide the theoretical basis for safe and effective use of storing suspending erythrocytes.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fifty blood samples were selected randomly from blood center in HebeiProvince, with detection of all indicators qualified. Each divided into 8 groups（A, B, C, D, E, F, G, H） which were stored at 4 ℃, heated at 37 ℃ for various time(10, 20, 30, 60, 120, 180 min) and 40 ℃ for 30 min for subsequent experiments. IL8 concentration in plasma was detected by adopt double antibody sandwich enzymelinked immunosorbent assay.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Heating could significantly upregulated IL8 level, and with timedependence pattern. There was no significant difference in plasma IL8 concentration between B and C group compared with A group(P＞005). Plasma levels of IL8 in D, E, F, G, H were significantly higher than those in the A group, the difference was statistically significant(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓There was no significant effect on plasma IL8 concentration in water bath at 37 ℃ within 30 min，and then gradually increased with time extended.

Key words: blood transfusion, erythrocyte immune functions, interleukin8

版权所有 © 《河北医科大学学报》编辑部
本系统由北京玛格泰克科技发展有限公司设计开发