关键词: 甲状腺功能亢进症, 妊娠, 子代, 肾功能, 大鼠

Abstract: ［Abstract］ Objective〖HTSS〗〓To observe the expressions of transforming growth factor 1(TGF1) and nuclear factor kappa B(NFκB) P65 in the renal interstitium of pregnant rats with hyperthyroidism and to investigate the relationship between hyperthyroidism in pregnancy and renal interstitial fibrosis in offspring.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fiftyfour SD rats aged 60 days were randomly divided into 3 groups, 18 rats in each group which were given continuous gavage with different doses of levothyroxine sodium to make a hyperthyroidism model. After pregnancy, the mother mice continued to be gavage until delivery. The pathological structure and damage of kidney in normal control group, mild hyperthyroidism group and severe hyperthyroidism group were observed. Western blot was also used to determine the expression of TGF1 and NFκB P65.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The serum levels of free triiodothyronine(FT3) and free thyroxine(FT4) in the severe hyperthyroidism newborn mouse group at 21 and 60 days were higher than those in the normal control newborn mouse group and the mild hyperthyroidism newborn mouse group, and the serum levels of thyroid stimulating hormone(TSH) in the severe hyperthyroidism newborn mouse group at 21 and 60 days were lower than those in the normal control newborn mouse group and the mild hyperthyroidism newborn mouse group. FT3 and FT4 were higher on day 60 than on day 21 in each group, and TSH was lower on day 60 than on day 21 in each group, with statistically significant differences(P＜005). TGF 1 and NFκB P65 in renal tubular interstitial cells were higher in the mild hyperthyroidism newborn mouse group and the severe hyperthyroidism newborn mouse group than in the normal control newborn mouse group at 0 d, 21 d and 60 d. The expression levels of TGF 1 and NFκB P65 in the 3 groups were higher at 21 d and 60 d than at 0 d and higher at 60 d than at 21 d, with statistically significant differences(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Maternal hyperthyroidism can cause renal interstitial fibrosis in offspring, which is related to the degree of hyperthyroidism. The early diagnosis and treatment of hyperthyroidism are of great significance for preventing renal interstitial fibrosis in offspring.

Key words: hyperthyroidism, pregnancy, offspring, renal function, rats