河北医科大学学报

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miR19a3p通过PTEN调节人胃癌细胞增殖水平#br#

  

  1. 1.河北省邢台市第一医院腹部外三科，河北 邢台 054001；2.河北省邢台市人民医院呼吸内科，河北 邢台 054001
  • 出版日期:2019-05-25 发布日期:2019-05-22
  • 作者简介:聂红峰（1976-），男，河北沙河人，河北省邢台市第一医院副主任医师，医学博士研究生，从事胃肠肿瘤诊治研究。

miR19a3p regulates the proliferation of gastric cancer cells via targeting PTEN#br#

  1. 1.The Third Department of Abdominal Surgery, the First Hospital of Xingtai， Hebei Province，
    Xingtai 054001, China; 2.Department of Respiratory Medicine, the People′s Hospital of
    Xingtai, Hebei Province, Xingtai 054001, China
  • Online:2019-05-25 Published:2019-05-22

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摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨miR19a3p通过下游靶基因PTEN调节人胃癌MKN28细胞增殖水平，从microRNA角度阐明胃癌细胞增殖的机制，为胃癌的防治提供新的思路。
〖HTH〗方法〖HTSS〗〖KG*2〗收集20例人胃癌组织和癌旁组织，实时荧光定量PCR方法分析miR19a3p表达水平；在人胃癌MKN28细胞中转染miR19a3p 类似物和miR19a3p 反义链，用实时荧光定量PCR方法分析miR19a3p表达水平，用MTT法检测细胞增殖水平；在人胃癌MKN28细胞中转染miR19a3p 类似物和miR19a3p 反义链，用Western blot方法分析PTEN蛋白水平；在人胃癌MKN28细胞中共转染miR19a3p 反义链和PTEN特异性的小RNA片段 （siRNAPTEN,siPTEN），用Western blot方法分析PTEN蛋白水平,用MTT法检测细胞增殖水平。
〖HTH〗结果〖HTSS〗〖KG*2〗胃癌组织中miR19a3p表达水平明显高于癌旁组织（P＜005）。转染miR19a3p类似物组MKN28细胞中miR19a3p水平和细胞增殖水平均明显高于阴性对照组（P＜005），转染miR19a3p 反义链组MKN28细胞中miR19a3p水平和细胞增殖水平均明显低于反义阴性对照组（P＜005）。转染miR19a3p类似物组与阴性对照组PTEN mRNA水平差异无统计学意义（P＞005），miR19a3p反义链组与反义阴性对照组PTEN mRNA水平差异无统计学意义（P＞005）。miR19a3p反义链组MKN28细胞增殖水平低于反义阴性对照组，PTEN siRNA组MKN28细胞增殖水平高于反义阴性对照组，miR19a3p反义链+PTEN siRNAMKN28细胞增殖水平低于PTEN siRNA组（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗在人胃癌MKN28细胞中miR19a3p通过调节下游靶基因PTEN影响细胞增殖。

关键词: 胃肿瘤, 微RNAs, 细胞增殖

Abstract: ［Abstract］〓Objective〖HTSS〗〓To discuss the effect of miR19a3p on the proliferation of human gastric cancer cell via targeting PTEN, which provided new ideas for the prevention and treatment of gastric cancer.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Gastric carcinoma tissue specimens and matched adjacent tissues were obtained from twenty patients who were identified as aggressive gastric adenocarcinoma. Realtime PCR was used to analyze the levels of miR19a3p in gastric carcinoma tissue specimens and matched adjacent tissues. miR19a3p mimic and miR19a3p inhibitor were transfected into MKN28 cells. Realtime PCR was used to analyze the level of miR19a3p. And MTT assay was used to determine the proliferation of MKN28 cell. miR19a3p mimic and miR19a3p inhibitor were transfected into MKN28 cells. Western blot was used to measure the protein level of PTEN. miR19a3p inhibitor and siRNAPTEN were transfected into MKN28 cells. Western blot was used to measure the protein level of PTEN. And MTT assay was used to determine the proliferation of MKN28 cell.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with adjacent tissues, miR19a3p expression was increased in gastric cancer tissues(P＜005). Compared with negative mimic control group, transfection with miR19a3p mimic increased miR19a3p expression(P＜005). Compared with negative inhibitor control group, transfection with miR19a3p inhibitor decreased miR19a3p expression(P＜005). The level of PTEN mRNA did not significantly change in MKN28 cells transfected with miR19a3p mimic(P＞005) or miR19a3p inhibitor(P＞005). The proliferation of MKN28 cells was inhibited in MKN28 cells transfected with miR19a3p inhibitor(P＜005) and was promoted in MKN28 cells transfected with siPTEN(P＜005). Compared with siPTEN group, the proliferation of MKN28 cells was decreased in MKN28 cells cotransfected with siPTEN and miR19a3p inhibitor(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓miR19a3p regulated the proliferation of human gastric cancer cells via targeting PTEN.

Key words: stomach neoplasms, microRNAS, cell proliferation

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