丹参提取物对肿瘤合并心肌缺血损伤的保护效应

doi:10.3969/j.issn.10073205.2019.06.004

河北医科大学学报

丹参提取物对肿瘤合并心肌缺血损伤的保护效应

1.河北大学附属医院中心实验室,河北省放化疗机制与规程研究重点实验室,河北保定 071000;2.河北医科大学
基础医学院生物化学与分子生物学教研室，河北石家庄 050017；3.河北医科大学第二医院血液内科，河北省
血液病重点实验室,河北石家庄 050000；4.河北医科大学第二医院内分泌科，河北石家庄 050000

出版日期:2019-06-25 发布日期:2019-06-19
作者简介:秦岩（1985-），女，河北沧州人，河北大学附属医院主管检验师，医学博士，从事肿瘤血管新生机制研究。
基金资助:
国家自然科学基金（31701238）；河北省中医药管理局科研计划项目（2018151）；河北省研究生创新资助项目（CXZZBS2017107）；河北医科大学第二医院科学研究基金（2h201842）

Protective effect of Salvia miltiorrhiza extract on tumor with myocardial ischemic injury

1.Department of Central Laboratory, Affiliated Hospital of Hebei University, Key Laboratory for
Fractionation Mechanisms and Procedures, Hebei Province, Baoding 071000, China; 2.Department of
Biochemistry and Molecular Biology, the School of Basic Medical Sciences, Hebei Medical University,
Shijiazhuang 050017, China； 3.Department of Endocrinology, the Second Hospital of Hebei
Medical University, Key Laboratory for Hematology of Hebei, Shijiazhuang 050000, China;
4.Department of Hematology, the Second Hospital of Hebei Medical
University, Shijiazhuang 050000, China

Online:2019-06-25 Published:2019-06-19

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗观察丹参提取物在肿瘤合并心肌缺血模型中的作用。
〖HTH〗方法〖HTSS〗〖KG*2〗30只雌性BALB/Cnu 4～6周龄裸鼠皮下接种乳腺癌MCF7细胞，制备移植瘤，选取成功移植瘤的18只小鼠随机分为3组，即肿瘤模型组、药物丹参酮ⅡA组、药物丹酚酸B组，每组6只。移植瘤第7天后，每组给予腹腔注射异丙肾上腺素2.5 mg/kg，连续注射7 d，制备肿瘤合并心肌缺血动物模型。另设正常对照组6只。取出肿瘤组织称重，心脏组织进行Masson′s Trichrome 染色。采用免疫组织化学方法检测各组心肌和肿瘤组织中CD34和血管性血友病因子(von Willebrand Factor, vWF)的表达，采用MTT方法观察丹参酮ⅡA对人脐静脉血管内皮细胞（human umbilical vein endothelial cells，HUVECs）及乳腺癌MCF7增殖活力的影响。
〖HTH〗结果〖HTSS〗〖KG*2〗肿瘤模型组心肌胶原纤维面积比较正常对照组明显增多；丹参酮ⅡA组及丹酚酸B组肿瘤重量和心肌胶原纤维面积比明显小于肿瘤模型组（P＜005）；在心肌组织中，丹参酮ⅡA组和丹酚酸B组CD34和vWF蛋白表达水平较肿瘤模型组明显升高(P＜005)；在肿瘤组织中，丹参酮ⅡA组和丹酚酸B组CD34和vWF蛋白表达水平较肿瘤模型组明显降低(P＜005)。体外实验结果显示，丹参酮ⅡA能够促进HUVEc增殖,抑制乳腺癌细胞增殖,均具有剂量依赖性。
〖HTH〗结论〖HTSS〗〖KG*2〗丹参酮ⅡA有保护心肌缺血和抑制乳腺癌细胞的生长的作用，可能与血管生成有关。

关键词: 心肌缺血, 乳腺肿瘤, 丹参提取物

Abstract: ［Abstract］ Objective〖HTSS〗〓To observe the role of Salvia miltiorrhiza extract in tumor with myocardial ischemia model.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirty female BALB/Cnu nude mice which were 4-6 week old were subcutaneously inoculated with breast cancer MCF7 cells to prepare transplanted tumors. Eighteen mice with successful transplanted tumors were randomly divided into three groups: tumor model group，tanshinone ⅡA group and salvianolic acid B group. After 7 days of transplantation, each group was injected with isoproterenol 2.5 mg/kg intraperitoneally for 7 days to establish an animal model of tumor with myocardial ischemia. Another 6 rats were set as normal control group. The tumor tissue was removed and weighed, and the heart tissue was subjected to Masson′s Trichrome staining. The expression of CD34 and von Willebrand Factor(vWF) in myocardium and tumor tissues was detected by immunohistochemistry. The effect of tanshinone ⅡA on human umbilical vein endothelial cells(HUVECs) and breast cancer MCF7 were observed by MTT.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The results showed that the area of myocardial collagen fibers in the tumor model group was significantly higher than that in the normal control group. The tumor weight and myocardial collagen fiber area in the tanshinone ⅡA group and salvianolic acid B group was significantly smaller than that in the the tumor model group(P＜005). In the myocardial tissue, the expression levels of CD34 and vWF protein in the tanshinone ⅡA group and the salvianolic acid B group were significantly higher than those in the tumor model group(P＜005) . In the tumor tissue, the expression levels of CD34 and vWF protein in tanshinone ⅡA group and in salvianolic acid B were significantly lower than those in the tumor model group(P＜005). In vitro experiments showed that tanshinone ⅡA can promote the proliferation of HUVECc and inhibit the proliferation of breast cancer cells in a dosedependent manner.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The effect of tanshinone ⅡA on protecting myocardial ischemia and inhibiting the growth of breast cancer cells may be related to angiogenesis.

Key words: myocardial ischemia, breast neoplasms, Salvia miltiorrhiza extract

秦〓岩1,2，杨高山2，张〓楠2，杨浩杰2，聂子元3，周〓静2,4*. 丹参提取物对肿瘤合并心肌缺血损伤的保护效应[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2019.06.004.

QIN Yan1,2, YANG Gaoshan2, ZHANG Nan2, YANG Haojie2, NIE Ziyuan3， ZHOU Jing2,4*. Protective effect of Salvia miltiorrhiza extract on tumor with myocardial ischemic injury[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2019.06.004.

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丹参提取物对肿瘤合并心肌缺血损伤的保护效应

Protective effect of Salvia miltiorrhiza extract on tumor with myocardial ischemic injury

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