河北医科大学学报

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miR122通过靶定LMNB2基因抑制肝癌细胞活性的研究

  

  1. 浙江省立同德医院普通外科，浙江 杭州 310012
  • 出版日期:2019-07-25 发布日期:2019-07-16
  • 作者简介:杨涛（1977-），男，河北衡水人，浙江省立同德医院副主任医师，医学硕士，从事肝胆外科疾病诊治研究。
  • 基金资助:
    河北省科技计划项目（16277705D）

miR122 inhibits the activity of liver cancer cells by targeting the LMNB2 gene

  1. Department of General Surgery, the Tongde Hospital of Zhejiang Province, Hangzhou 310012， China
  • Online:2019-07-25 Published:2019-07-16

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摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨miR122在肝癌进展中的作用及其机制。
〖HTH〗方法〖HTSS〗〖KG*2〗应用荧光素酶报告基因实验和Western blot实验证实miR122对LMNB2基因的调控作用。应用菌落形成试验和Transwell细胞侵袭实验分析LMNB2基因对肝癌细胞生物活性的影响。
〖HTH〗结果〖HTSS〗〖KG*2〗野生型转染miR122 mimics后荧光素酶活性弱于对照组(P＜005)；突变体转染miR122 mimics后荧光素酶活性与对照组差异无统计学意义（P＞005）。在肝癌细胞Hep3B中沉默miR122表达，LMNB2表达水平明显高于对照组（P＜005）。在肝癌细胞SMMC7721中过表达miR122后LMNB2表达水平明显低于对照组（P＜005）。在肝癌细胞Hep3B中过表达LMNB2，肝癌细胞的侵袭及迁移能力明显强于对照组（P＜005）；在肝癌细胞SMMC7721中沉默LMNB2表达，肝癌细胞的侵袭及迁移能力明显低于对照组（P＜005）。在肝癌细胞Hep3B中过表达LMNB2，肝癌细胞的增殖能力明显强于对照组（P＜005）；在肝癌细胞SMMC7721中沉默LMNB2表达，肝癌细胞的增殖能力明显低于对照组（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗 miR122可以通过靶定LMNB2抑制肝癌细胞的生物活性，其可以作为肝细胞肝癌早期诊断指标和治疗的靶点。

关键词: 肝肿瘤, 微RNAs, 基因表达

Abstract: ［Abstract］〓Objective〖HTSS〗〓To investigate the role and mechanism of miR122 in the progression of liver cancer.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The regulatory effect of miR122 on LMNB2 gene was confirmed by luciferase reporter gene assay and Western blot. Then the effect of LMNB2 gene on the bioactivity of hepatocellular carcinoma（HCC） cells was analyzed by colony formation assay and transwell cell invasion assay .
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The luciferase activity of the wildtype mir122 mimics was lower than that of the control group(P＜005). The luciferase activity of the mutant transfected with mir122 mimics was not significantly different from that of the control group(P＞005). The expression of mir122 was silenced in hepatocellular carcinoma cells Hep3B, and the expression level of LMNB2 was significantly higher than that of the control group(P＜005). LMNB2 expression level was significantly lower than that of the control group after overexpression of mir122 in liver cancer SMMC7721 cells(P＜005). LMNB2 was overexpressed in hepatocellular carcinoma cells Hep3B, and the invasion and migration ability of hepatocellular carcinoma cells was significantly stronger than that of the control group(P＜005). The LMNB2 expression was silenced in SMMC7721 HCC cells, and the invasion and migration ability of HCC cells was significantly lower than that of the control group(P＜005). LMNB2 was overexpressed in hepatocellular carcinoma cells Hep3B, and the proliferation capacity of hepatocellular carcinoma cells was significantly stronger than that of the control group(P＜005). The LMNB2 expression was silenced in SMMC7721 hepatocellular carcinoma cells, and the proliferation capacity of hepatocellular carcinoma cells was significantly lower than that of the control group(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓miR122 can inhibit the biological activity of HCC cells by targeting LMNB2, which can be used as an early diagnostic indicator and therapeutic target for HCC.

Key words: liver neoplasms, microRNAs, gene expression

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