HIF-1α和SDF-1在分化型甲状腺癌中的表达及意义#br#

doi:10.3969/j.issn.10073205.2019.09.018

河北医科大学学报

HIF-1α和SDF-1在分化型甲状腺癌中的表达及意义#br#

1.四川省自贡市第一人民医院核医学科，四川自贡 643000；2.陆军军医大学（第三军医大学），军事预防医学系热带医学教研室，重庆 400038

出版日期:2019-09-25 发布日期:2019-09-16
作者简介:周丹(1982-)，男，四川自贡人，四川省自贡市第一人民医院主治医师，医学学士，从事核医学研究。
基金资助:
国家自然科学基金项目（81773396）

Expression and significance of HIF1α and SDF1 in differentiated thyroid carcinoma#br#

1.Department of Nuclear Medicine, Zigong First People′s Hospital， Sichuan Province, Zigong 643000, China；
2.Department of Tropical Medicine, College of Military Preventive Medicine, Army Medical University
(Third Military Medical University), Chongqing 400038, China

Online:2019-09-25 Published:2019-09-16

摘要/Abstract

摘要： ［摘要］
〖HTH〗目的〖HTSS〗〖KG*2〗探讨缺氧诱导因子 1α（hypoxia inducible factor1α，HIF1α）和基质细胞衍生因子1(stromal cell derived factor 1，SDF1)在分化型甲状腺癌中的表达及意义。
〖HTH〗方法〖HTSS〗〖KG*2〗取分化型甲状腺癌（differentiated thyroid carcinoma，DTC）患者手术切除的癌和癌旁组织，根据有无转移将患者分为原位癌组、转移癌组，另设对照组（癌旁组织）。用实时荧光定量核酸扩增检测系统检测组织中HIF1α与SDF1 mRNA表达量。同时体外培养SW579细胞，干扰HIF1α检测细胞增殖、侵袭能力以及SDF1转录和蛋白表达；检测外源性加100 ng/mL的SDF1对SW579细胞SDF1转录、增殖和侵袭能力的影响，干扰HIF1α回补SDF1后检测人SW579细胞增殖和侵袭能力。
〖HTH〗结果〖HTSS〗〖KG*2〗DTC患者切除组织中原位癌和转移癌HIF1α和SDF1转录表达水平均显著高于癌旁组织（对照组），转移癌组中HIF1α和SDF1表达水平又高于原位癌组（P＜0.05）； HIF1αsiRNA组和HIF1αsiRNA+SDF1组SDF1转录水平低于siNC组和siNC+SDF1组，同时HIF1αsiRNA组SDF1蛋白水平也低于SiNC组（P＜0.05）。siNC+SDF1组细胞增殖和细胞侵袭力均高于siNC组，HIF1αsiRNA组细胞增殖和细胞侵袭力均低于siNC组和siNC+SDF1组，HIF1αsiRNA+SDF1组细胞增殖和细胞侵袭力低于siNC+SDF1组，但高于HIF1αsiRNA组，差异均有统计学意义（P＜0.05）。
〖HTH〗结论〖HTSS〗〖KG*2〗HIF1α和SDF1在DTC肿瘤组织中高表达，且HIF1α通过调控SDF1在DTC的转移过程中发挥重要的作用。

关键词: 甲状腺肿瘤, 缺氧诱导因子1, &alpha, 亚基, 趋化因子CXCL12

Abstract: ［Abstract］ Objective〖HTSS〗To investigate the expression and significance of hypoxia inducible factor1 α(HIF1α) and stromal cell derived factor 1(SDF1) in differentiated thyroid carcinoma.
〖HTH〗〖WTHZ〗Methods〖HTSS〗According to the presence or absence of metastasis, the patients with differentiated thyroid carcinoma（DTC）were divided into three groups: carcinoma in situ group, metastatic cancer group and control group (paracancerous tissue). The expression of HIF1α and SDF1 in tissues was detected by qPCR. At the same time, SW579 cells were cultured in vitro, knocking out HIF1α to detect the transcription and protein expression of SDF1 and cell proliferation and invasion ability. The transcription of SDF1 and cell proliferation and invasion ability of SW579 cells were detected by exogenous addition of SDF1 100 ng/mL，and the proliferation and migration ability of SW579 cells was detected with replenishing SDF1.
〖HTH〗〖WTHZ〗Results〖HTSS〗The transcriptional expression levels of HIF1α and SDF1 in the excised tissues of DTC patients were significantly higher than those in the adjacent tissues, and the expression levels in the metastasis group were significantly higher than those in the in situ cancer group. The transcription level of SDF1 in HIF1αsiRNA group and HIF1αsiRNA+SDF1 group was lower than that in siNC group and siNC+SDF1 group(P＜0.05). At the same time, the level of SDF1 protein in the HIF1αsiRNA group was also lower than that in the SiNC group. The cell proliferation and cell invasion of siNC+SDF1 group were higher than those of siNC group. The cell proliferation and cell invasion of HIF1αsiRNA group were lower than siNC group and siNC+SDF1 group. The cell proliferation and cell invasion of HIF1αsiRNA+SDF1 group were lower than those of siNC+SDF1 group, but higher than HIF1αsiRNA group, the difference was statistically significant(P＜0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗HIF1α and SDF1 are highly expressed in DTC tumors, and HIF1α plays an important role in the metastasis of DTC by regulating SDF1.

Key words: thyroid neoplasms, hypoxiainducible factor 1, alpha subunit, chemokine CXCL12

周丹1，刘军2，罗珍2，申婷婷2，唐嘉励1*. HIF-1α和SDF-1在分化型甲状腺癌中的表达及意义#br#[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2019.09.018.

ZHOU Dan1, LIU Jun2, LUO Zhen2, SHEN Tingting2, TANG Jiali1*. Expression and significance of HIF1α and SDF1 in differentiated thyroid carcinoma#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2019.09.018.

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HIF-1α和SDF-1在分化型甲状腺癌中的表达及意义#br#

Expression and significance of HIF1α and SDF1 in differentiated thyroid carcinoma#br#

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