河北医科大学学报 ›› 2023, Vol. 44 ›› Issue (9): 998-1005.doi: 10.3969/j.issn.10073205.2023.09.002

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QTRT1表达与肾透明细胞癌预后及肿瘤免疫的相关性

  

  1. 昆明医科大学第二附属医院病理科，云南 昆明 650032
  • 出版日期:2023-09-25 发布日期:2023-10-12
  • 作者简介:王天云（1996-），男，白族，昆明医科大学第二附属医院硕士研究生，从事肿瘤疾病诊治研究。
  • 基金资助:
    云南省教育厅科学研究基金项目（2022J0219）

QTRT1 expression is associated with prognosis and tumor immunity in kidney renal clear cell carcinoma

  1. Department of Pathology, the Second Affiliated Hospital of Kunming Medical University, Yunnan Province， Kunming 650032, China

  • Online:2023-09-25 Published:2023-10-12

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摘要： 目的  通过生物信息分析的方法探求队列tRNA-核糖基转移酶 1（queuine tRNA-ribosyl transferase 1，QTRT1）的表达与肾透明细胞癌（kidney renal clear cell carcinoma，KIRC）预后及肿瘤免疫间的关系。
方法  通过R软件、TCGA数据库、GEO数据库、HPA数据库、STRING数据库、GENEMAIN数据库、GSEA软件、TIMER2.0数据库、Sanger box 3.0在线平台、GEPIA2平台等多个软件和在线工具进行研究。并辅以实时荧光定量多聚核苷酸链式反应（real-time quartiative polymerase chain reaction,qRT-PCR）进行细胞实验验证。
结果  通过对TCGA的535例癌和72例正常组织进行秩和检验显示，KIRC组织中QTRT1的表达显著高于正常组织（P＜0.05），KIRC细胞780O和Caki2中QTRT1的mRNA表达强度明显强于正常肾小管上皮细胞HK2（P＜0.05），QTRT1的mRNA表达在T分期和临床分期的不同组别间比较差异有统计学意义（P＜0.05），年龄、分级、M分期和QTRT1表达均与KIRC的OS显著相关，年龄、分级、M分期和QTRT1表达都可以作为KIRC中OS的独立预后因素（P＜0.05）。共有19种与QTRT1最具相互作用关系的基因被展示。同时利用STRING平台构建PPI网络，并显示了与QTRT1相互作用的10种蛋白质。利用Sangerbox得到了QTRT1与肿瘤突变负荷Tumor Mutation Burden（TMB）间的关系，在KIRC中QTRT1与TMB呈现正相关（P＜0.05）。
结论  QTRT1在KIRC中显著上调，它的上调是KIRC患者的不良预后信号。QTRT1与KIRC的免疫细胞浸润、免疫检查点等多个免疫因素联系紧密，是免疫治疗的潜在作用靶点。


关键词: 癌, 肾细胞；QTRT1；免疫

Abstract: Objective  To investigate the relationship between the expression of Queuine tRNAribosyl transferase 1  (QTRT1) and the prognosis and tumor immunity in kidney renal clear cell carcinoma (KIRC) by means of bioinformatics analysis. 
Methods  The research was carried out through multiple software and online tools such as R software, TCGA database, GEO database, HPA database, STRING database, GENEMAIN database, GSEA software, TIMER2.0 database, Sanger box 3.0 online platform, and GEPIA2 platform, supplemented by qRT-PCR for cell experiment verification. 
Results  By conducting rank sum tests on 535 patients with cancer and 72 normal tissues in TCGA, it was found that the expression of QTRT1 in KIRC tissue was significantly higher than that in normal tissue (P＜0.05). The mRNA expression intensity of QTRT1 in 780-O and Caki2 of KIRC cells was significantly stronger than that in normal renal tubular epithelial cell HK2 (P＜0.05). The mRNA expression of QTRT1 showed statistical significance in different groups of T stage and clinical stage (P＜0.05). Age, grade, M stage, and QTRT1 expression were all significantly correlated with OS of KIRC. Age, grade M-stage and QTRT1 expression could serve as independent prognostic factors for OS in KIRC (P＜0.05). A total of 19 genes with the most significant interaction with QTRT1 were displayed. Simultaneously, a PPI network was constructed using the STRING platform, and 10 proteins interacting with QTRT1 were displayed. The relationship between QTRT1 and Tumor Mutation Burden (TMB) was obtained using Sangerbox, and there was a positive correlation between QTRT1 and TMB in KIRC (P＜0.05). 
Conclusion  QTRT1 is significantly upregulated in KIRC, and its upregulation is a poor prognostic signal in KIRC patients. QTRT1 is closely related to multiple immune factors such as immune cell infiltration, and immune checkpoints in KIRC, and is a potential target for immunotherapy. 


Key words: carcinoma, renal cell； QTRT1, immunity

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