关键词: 肝肿瘤, 肿瘤转移, 血液

Abstract: Objective To explore the blood supply of rabbit liver metastasis(LM)based on its model by using CT perfusion and immunohistochemical method.Methods VX2 tumor strains were injected in the spleen of 35 New Zealand white rabbits.The LM was confirmed by using CT scan,and then the CT perfusion was performed.The rabbit LM was separated.The immunohistochemical staining was used to observe the expressions of CD34,intercellular cell adhesion moleculer-1(ICAM-1)and α-smooth muscle actin(α-SMA).Results When the average size of LM neoplasm reached approximately to(315.76±24.78)μm,no expressions of ICAM-1, CD34 andα-SMA could be observed,and the ICAM-1 positive cells in the tumor were connected with the normal sinusoidal.CD34 and ICAM-1 positive cells and someα-SMA positive cells could be seen in the size of LM(632.58± 35.46)μm neoplasm,and the ICAM-1 positive cells in the tumor were connected with the normal sinusoidal.The CD34 and α-SMA positively expressed in the size of LM(1 056.32± 124.67)μm neoplasm,and the ICAM-1 positive cells were further reduced;only CD34 and α-SMA positive cells expressed in the average size of LM(2 183.15± 167.32)and LM(5 326.00± 172.51)μm neoplasms,ICAM-1 staining disappeared or only a few ICAM-1 positive cells could be found around the sinusoidal tumor or normal sinusoidal;the size of 1.0 cm metastases expressed the CD34,α-SMA and ICAM-1,and the staining was similar to the previous stage.LM showed ring staining.CT perfusion of LM of hepatic arterial fraction,hepatic arterial perfusion,hepatic arterial index were greater than the perlesion liver tissue,while portal vein perfusion and portal venous perfusion index were less than the perlesion liver tissue. Conclusion The early stage of rabbit LM was supplied by portal vein blood,with the increase of LM,the hepatic artery increased gradually,the portal vein blood supply decreased gradually.When LM developed to a certain stage,only the hepatic artery,portal vein does not participate in the blood supply.

Key words: liver neoplasms, neoplasm metastasis, blood