河北医科大学学报 ›› 2022, Vol. 43 ›› Issue (7): 768-774.doi: 10.3969/j.issn.1007-3205.2022.07.006

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SPARC蛋白在乳腺癌组织中的表达及临床意义

  

  1. 河北医科大学第四医院乳腺中心，河北 石家庄 050011 

  • 出版日期:2022-07-25 发布日期:2022-07-26
  • 作者简介:张摇（1993-），女，河北石家庄人，河北医科大学第四医院医师，医学硕士，从事乳腺良恶性疾病诊治研究。

Expression and clinical significance of SPARC protein in breast cancer

  1. Department of Breast Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China

  • Online:2022-07-25 Published:2022-07-26

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摘要： 目的 半胱氨酸的分泌性酸性蛋白(secreted protein acidic and rich in cysteine，SPARC)蛋白在肿瘤生长、侵袭和转移中起重要作用，探讨SPARC在乳腺癌组织的表达及其与预后的关系。
方法 随机收集河北医科大学第四医院原发性乳腺癌患者术后标本122例以及正常乳腺组织标本38例，应用免疫组织化学方法检测两组标本中SPARC蛋白表达水平，分析其与患者临床病理资料的关系，采用Kaplan-Meier 法、Log-rank 检验、COX回归模型进行生存分析和危险因素分析。
结果 SPARC蛋白在乳腺癌组织中上皮细胞的阳性率高于正常乳腺组织（24.6% vs. 7.9%），差异有统计学意义（P＜0.05）；两组间质细胞的阳性率分别为54.9%、68.4%，差异无统计学意义（P＞0.05）。将肿瘤组织中上皮细胞的着色定义为阳性。乳腺癌组织中SPARC蛋白的表达与患者年龄、肿物直径、淋巴结状态、TNM分期、脉管瘤栓、组织学分级、分子分型、雌激素受体（estrogen receptor，ER）、人表皮生长因子受体２（human epidermal growth factor receptor 2，HER2）状态等均不具有相关性（P＞0.05），与病理类型、孕激素受体（progesterone receptor，PR）状态相关（P＜0.05）。SPARC蛋白表达阳性与阴性的乳腺癌患者总生存（overall survival，OS）差异有统计学意义(P＜0.05)，无病生存（disease-free survival ，DFS）差异无统计学意义（P＞0.05）。SPARC蛋白表达不是影响患者OS的独立预后因素 ［P=0.052，HR=0.142(0.019~1.060)］。
结论 SPARC在乳腺癌组织上皮细胞中的表达明显高于正常乳腺组织，且与病理类型和PR状态相关。SPARC蛋白的表达与患者OS相关，可能是影响患者死亡的独立预后因素，可降低患者的死亡风险。


关键词: 乳腺肿瘤, SPARC蛋白, 免疫组织化学

Abstract: Objective Secreted proteinacidic and rich in cysteine(SPARC) plays an important role in tumor growth, invasion and metastasis. This article explores the expression of SPARC in breast cancer tissues and its relationship with prognosis. 
Methods In total,122 postoperative specimens of primary breast cancer patients from the Fourth Hospital of Hebei Medical University and 38 normal breast tissue specimens were randomly collected. Chi-square test was used to analyze the relationship of SPARC with the clinicopathological data of patients. Kaplan-Meier method, Log-rank test and COX regression model were used to analyze survival and risk factors. 
Results The positive rate of SPARC in breast cancer tissues was higher than that in normal breast tissues(24.6% vs. 7.9%), and the difference was statistically significant(P＜0.05). The positive rates of interstitial cells in the two groups were 54.9% and 68.4% respectively, and the difference was not statistically significant(P＞0.05). The staining of epithelial cells in tumor tissue was defined as positive. The expression of SPARC in breast cancer tissues was not related to patient age, tumor diameter, lymph node status, TNM stage, vascular tumor thrombus, histological grade, molecular typing, estrogen receptor(ER), and human epidermal growth factor receptor 2(HER2) status(all P＞0.05), but related to pathological type and progesterone receptor(PR) status(P＜0.05). The difference in overall survival(OS) of breast cancer patients with positive and negative SPARC protein expression was statistically significant(P＜0.05), and disease-free survival(DFS) was not statistically different(P＞0.05). SPARC protein expression was not an independent prognostic factor for OS in patients［P=0.052, HR=0.142(0.019-1.060)］. 
Conclusion The expression of SPARC in epithelial cells of breast cancer tissue is significantly higher than normal breast tissue and is related to pathological type and PR status. The expression of SPARC is related to the patient′s OS, which may be an independent prognostic factor that affects the patient′s death and can reduce the patient′s risk of death.


Key words: breast neoplasms, SPARC, immunohistochemistry

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