河北医科大学学报 ›› 2023, Vol. 44 ›› Issue (4): 376-381.doi: 10.3969/j.issn.1007-3205.2023.04.002

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沉默ANGPTL2对氧-葡萄糖剥夺/复氧诱导的HT22细胞氧化应激及凋亡的影响

  

  1. 电子科技大学医学院附属绵阳医院，绵阳市中心医院急诊科，四川 绵阳 621000

  • 出版日期:2023-04-20 发布日期:2023-04-20
  • 作者简介:刘佳雨（1989-），女，羌族，四川平武人，电子科技大学医学院附属绵阳医院主治医师,医学硕士，从事急诊科疾病诊治研究。
  • 基金资助:
    四川省卫生健康委员会科研项目（19PJ113）

Effects of ANGPTL2 silencing on oxidative stress and apoptosis of HT22 cells induced by oxygen-glucose deprivation/reoxygenation

  1. Department of Emergency, the Central Hospital of Mianyang City, Mianyang Hospital Affiliated to School of Medicine, University of Electronic Science and Technology of China, Sichuan Province, Mianyang 621000, China

  • Online:2023-04-20 Published:2023-04-20

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摘要： 目的 探究沉默血管生成素样蛋白2（angiopoietin-like protein 2,ANGPTL2)对氧-葡萄糖剥夺/复氧（oxygen-glucose deprivation/reoxygenation，OGD/R）诱导的神经元细胞（hippocampal neuronal cell line，HT22）细胞氧化应激及凋亡的影响。
方法 通过OGD/R处理建立小鼠海马HT22细胞模型。通过实时荧光定量聚合酶链反应、细胞计数试剂盒、末端标记法及试剂盒检测ANGPTL2 mRNA的含量、细胞活力、细胞凋亡、细胞乳酸盐脱氢酶（lactate dehydrogenase，LDH)水平及细胞氧化应激。通过蛋白质印迹检测各种蛋白质含量。
结果 与对照组比较，模型组ANGPTL2 mRNA表达水平、LDH、凋亡率、ROS、MDA、核苷酸寡聚化结构域样受体家族3（nucleotide oligomeric domain-like receptor family 3，NLRP3）及凋亡相关斑点样蛋白（apoptosis-associated speck-like protein containing a card，ASC）、核因子E2相关因子2（nuclear factor E2 related factor 2，Nrf2）及血红素加氧酶1（heme oxygenase-1，HO-1）蛋白表达显著升高（P＜0.05），细胞活力、SOD显著降低（P＜0.05）。与OGD/R+si-con组比较，OGD/R+si-ANGPTL2组ANGPTL2 mRNA表达水平、LDH、凋亡率、ROS、MDA、NLRP3、ASC蛋白表达显著降低（P＜0.05），细胞活力、SOD及Nrf2、HO-1蛋白表达显著升高（P＜0.05）。
结论 沉默ANGPTL2通过调控Nrf2/HO-1信号通路减轻OGD/R诱导的氧化应激及NLRP3炎性小体介导的神经元凋亡。


关键词: 血管生成素样蛋白2, 氧-葡萄糖剥夺/复氧, 氧化应激

Abstract: Objective To explore the effects of angiopoietin-like protein 2 (ANGPTL2) silencing on oxidative stress and apoptosis of hippocampal neuronal cell line HT22 cells induced by oxygen-glucose deprivation/reoxygenation (OGD/R). 
Methods The mouse HT22 cell model was established by OGD/R treatment. ANGPTL2 mRNA level, cell viability, cell apoptosis, lactate dehydrogenase (LDH) level, and cellular oxidative stress were determined by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), cell count kit, terminal labeling and kit. The levels of various proteins were detected by Western blotting. 
Results Compared with the control group, ANGPTL2 mRNA expression level, LDH, apoptosis rate, reactive oxygen species (ROS), malondialdehyde (MDA), nucleotide oligomeric domain-like receptor family 3 (NLRP3), apoptosis-associated speck-like protein containing a card (ASC), nuclear factor E2 related factor 2 (Nrf2) and protein expressions of heme oxygenase-1 (HO-1) were significantly increased (P＜0.05), while cell viability and super oxide dismutase (SOD) were significantly decreased (P＜0.05). Compared with OGD/R+si-con group, ANGPTL2 mRNA expression level, LDH, apoptosis rate, ROS, MDA, NLRP3 and ASC protein expression were significantly decreased in OGD/R+ Si-Con group (P＜0.05), while cell viability, and SOD as well as Nrf2 and HO-1 protein expression were significantly increased (P＜0.05). 
Conclusion  Silencing ANGPTL2 alleviates OGD/R-induced oxidative stress and NLRP3 inflammasome mediated neuronal apoptosis by regulating Nrf2/HO-1 signaling pathway. 


Key words: angiopoietin-like protein 2, oxygen-glucose deprivation/reoxygenation, oxidative stress

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