河北医科大学学报 ›› 2024, Vol. 45 ›› Issue (10): 1219-1225.doi: 10.3969/j.issn.1007-3205.2024.10.015

• • 上一篇    下一篇

TGF-β1、iRoot BP Plus对盖髓效果影响的实验研究

  

  1. 河北医科大学口腔医院口腔内科,河北省口腔医学重点实验室，河北省口腔疾病临床医学研究中心，河北 石家庄 050017

  • 出版日期:2024-10-25 发布日期:2024-10-15
  • 作者简介:许明明（1997-），女，安徽合肥人，河北医科大学口腔医院医师,医学硕士,从事口腔内科疾病诊治研究。
  • 基金资助:
    河北省政府资助临床优秀人才项目（361029）；河北省省级科技计划资助（20377761D）；河北省医学科学重点研究课题计划（20210047）

Experimental study of the effect of TGF-β1 and iRoot BP Plus on pulp capping

  1. Department of Oral Medicine,Hospital of Stomatology of Hebei Medical University, Hebei Provincial Key 
    Laboratory of Stomatology, Hebei Provincial Clinical Medical Research Center for 
    Oral Diseases, Shijiazhuang 050017, China

  • Online:2024-10-25 Published:2024-10-15

HTML

PDF (PC)

55

摘要： 目的 探讨转化生长因子β1（transforming growth factor-β1，TGF-β1）和iRoot BP Plus联合盖髓的可行性，以期为提高活髓保存成功率提供实验参考。
方法 获取4周龄雄性SD大鼠的上、下颌第一磨牙共40颗，截根，保留第一磨牙牙冠及冠髓组织，随机分为4组进行盖髓：对照组、iRoot BP Plus组、TGF-β1组、iRoot BP Plus+TGF-β1组。将盖髓术后的实验牙样本移植入6周龄雄性BALB/c裸鼠背部两侧的囊袋中。培养6周后，处死裸鼠并取出样本，进行HE染色，分析评价样本牙髓炎症反应程度和修复性牙本质或矿化组织形成情况；免疫组织化学检测牙本质基质蛋白1（dentin matrix protein 1，DMP-1）在成牙本质细胞中的表达情况。
结果 iRoot BP Plus组、TGF-β1组、iRoot BP Plus+TGF-β1组牙髓炎症反应程度、修复性牙本质或矿化组织形成程度优于对照组(P＜0.05)；iRoot BP Plus+TGF-β1组修复性牙本质或矿化组织形成程度明显优于iRoot BP Plus组、TGF-β1组(P＜0.05)。iRoot BP Plus组、TGF-β1组、iRoot BP Plus+TGF-β1组DMP-1在成牙本质细胞中的表达明显强于对照组，iRoot BP Plus+TGF-β1组DMP-1在成牙本质细胞中的表达明显强于iRoot BP Plus组和TGF-β1组(P＜0.05)。
结论 iRoot BP Plus、TGF-β1均能促进修复性牙本质或矿化组织的形成，iRoot BP Plus与TGF-β1联合应用于直接盖髓术时，形成修复性牙本质或矿化组织的能力优于单独使用iRoot BP Plus或TGF-β1。


关键词: 牙髓复盖术, 转化生长因子β1, iRoot BP Plus

Abstract: Objective To investigate the feasibility of transforming growth factor β1 (TGF-β1) combined with iRoot BP Plus for pulp capping, so as to provide experimental reference for improving the success rate of vital pulp preservation. 
Methods A total of 40 upper and mandibular first molars of 4-week-old male SD rats were obtained, their roots were cut off, and the crown and crown pulp tissues of the first molar were retained. They were randomly divided into four groups for pulp capping: the control group, iRoot BP Plus group, TGF-β1 group, and iRoot BP Plus+TGF-β1 group. The experimental teeth after pulp capping were transplanted into the back pockets of 6-week-old male BALB/C nude mice. At 6 weeks after culture, the nude mice were killed and the samples were stained with HE to evaluate the degree of pulpal inflammation and the formation of reparative dentin or mineralized tissue. The expression of dentin matrix protein 1 (DMP-1) in odontoblasts was detected by immunohistochemistry. 
Results The degree of pulp inflammation and the formation of reparative dentin or mineralized tissue in iRoot BP Plus group, TGF-β1 group, and iRoot BP Plus+TGF-β1 group were better than those in the control group (P＜0.05). The degree of formation of reparative dentin or mineralized tissue in iRoot BP Plus+TGF-β1 group was significantly higher than that in iRoot BP Plus group and TGF-β1 group (P＜0.05). The expression of DMP-1 in odontoblasts of iRoot BP Plus group, TGF-β1 group, and iRoot BP Plus+TGF-β1 group was significantly stronger than that of the control group, and the expression of DMP-1 in odontoblasts of iRoot BP Plus+TGF-β1 group was significantly stronger than that of iRoot BP Plus group and TGF-β1 group (P＜0.05). 
Conclusion iRoot BP Plus and TGF-β1 can promote the formation of reparative dentin or mineralized tissue. iRoot BP Plus combined with TGF-β1 can be used in direct pulp capping, and the ability to form reparative dentin or mineralized tissue was superior to iRoot BP Plus or TGF-β1 alone. 


Key words: dental pulp capping, transforming growth factor β1, iRoot BP Plus

版权所有 © 《河北医科大学学报》编辑部
本系统由北京玛格泰克科技发展有限公司设计开发