河北医科大学学报

• 论著 • 上一篇    下一篇

维生素D受体基因Apa1、Taq1多态性与肺鳞癌易感性的关系

  

  1. .河北省秦皇岛市第一医院肿瘤科，河北 秦皇岛 066000；2.承德医学院研究生院，河北 承德 067000
  • 出版日期:2017-01-25 发布日期:2017-02-08
  • 作者简介:杨雁鸿(1966-)，女，河北秦皇岛人，河北省秦皇岛市第一医院主任医师，医学硕士，从事恶性肿瘤诊治研究。
  • 基金资助:
    秦皇岛市科学技术研究与发展计划(201101A259)

Association of vitamin D receptor gene Apa1 and Taq1 polymorphisms with susceptibility to lung squamous cell carcinoma

  1. 1.Department of Oncology, the First Hospital of Qinhuangdao City, Hebei Province, Qinhuangdao
    066000, Chian; 2.Graduate School, Chengde Medical College, Chengde 067000, China
  • Online:2017-01-25 Published:2017-02-08

HTML

PDF (PC)

421

摘要： 摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨维生素D受体（vitamin D receptor,VDR）Apa1、Taq1两基因位点的多态性与肺鳞癌易感性之间的关系。
〖HTH〗方法〖HTSS〗〖KG*2〗采集288例肺鳞癌患者和284例健康对照个体肘静脉血5 mL，采用聚合酶链反应-限制性片段长度多态性(polymerase chain reactionrestriction fragment length polymorphism,PCRRFLP)技术方法，对VDR Apa1、Taq1两基因位点行基因型检测。
〖HTH〗结果〖HTSS〗〖KG*2〗VDR Taq1位点基因多态性是肺鳞癌发生的危险因素，其中tt+Tt基因型携带者发生肺鳞癌的风险是TT基因型携带者的0.556倍(OR=0.556，95%CI=0.393~0.785)。按是否吸烟分层分析：吸烟人群中，Taq1位点基因多态性在病例组与对照组间差异有统计学意义，tt+Tt基因型携带者发生肺鳞癌的风险是TT基因型携带者的0.049倍 (OR=0.049，95%CI=0.259~0.929)；t等位基因携带者发生肺鳞癌的风险是T等位基因携带者的0.444倍(OR=0.444，95%CI=0.241~0.817)。而VDR Apa1位点多态性与肺鳞癌易感性无相关性。
〖HTH〗结论〖HTSS〗〖KG*2〗VDR Taq1基因多态性与肺鳞癌的遗传易感性相关，tt+Tt基因携带者肺鳞癌发病风险降低；t等位基因与患肺鳞癌风险呈负相关；吸烟者中tt＋Tt基因携带者肺鳞癌的发病风险显著降低。

关键词: 肺肿瘤, 维生素D, 基因

Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the relationship between polymorphism of vitamin D receptor（VDR） gene(including Apa1, Taq1) and susceptibility of squamous cell carcinoma of lung cancer(SCC).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The blood samples were collected from 288 patients with SCC and 284 healthy individuals. Leukocyte DNA from peripheral blood was extracted. The method of polymerase chain reactionrestriction fragment length polymorphism(PCRRFLP) was applied to test the genotype of Apa1, Taq1 in VDR gene.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓VDR Taq1 gene polymorphisms is the risk factor of SCC. The SCC risk of tt+Tt genotype carriers is 0.556 times than the TT genotype carriers(OR=0.556， 95%CI=0.393-0.785). Based on stratification analysis of smoking: In smoking group, there is statistical significance in Taq1 gene polymorphisms. The SCC risk of tt+Tt genotype carriers is 0.049 times than the TT genotype carriers(OR=0.049， 95%CI=0.259-0.929). There is statistical significance in t allele and T allele. The SCC risk of t allele carriers is 0.444 times than the T allele carriers(OR=0.444， 95%CI=0.241-0.817).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Taq1 polymorphism is related to genetic susceptibility of SCC. The tt+Tt gene reduces the risk of SCC. The t allele is negatively correlated with the risk of SCC. Smokers with tt+Tt gene are significantly reduced the risk of SCC.

Key words: lung neoplasms, vitamin D, gene

版权所有 © 《河北医科大学学报》编辑部
本系统由北京玛格泰克科技发展有限公司设计开发