Hh信号通路相关因子Shh、Ptch-1和Gli-1在前列腺癌中的表达及意义

doi:10.3969/j.issn.1007-3205.2022.07.008

河北医科大学学报 ›› 2022, Vol. 43 ›› Issue (7): 781-786.doi: 10.3969/j.issn.1007-3205.2022.07.008

Hh信号通路相关因子Shh、Ptch-1和Gli-1在前列腺癌中的表达及意义

河北中石油中心医院泌尿外科，河北廊坊 065000

出版日期:2022-07-25 发布日期:2022-07-26
作者简介:张航(1991-),男，河北肥乡人，河北中石油中心医院主治医师，医学硕士，从事泌尿外科疾病诊治研究。
基金资助:
廊坊市科技支撑计划项目（2019013002）

Expression and significance of Hh signaling pathway-related factors Shh, Ptch-1 and Gli-1 in prostate cancer

Department of Urological Surgery, Hebei CNPC Central Hospital, Langfang 065000, China

Online:2022-07-25 Published:2022-07-26

摘要/Abstract

摘要： 目的探讨刺猬信号通路（hedgehog,Hh）信号通路相关因子Sonic刺猬信号通路(Sonic hedgehog,Shh）、Patched-1（Ptch-1）、脑胶质瘤相关癌基因1(glioma related oncogene homology-1，Gli-1）在前列腺癌中的表达及意义。
方法收集60例前列腺癌、前列腺癌旁正常组织标本，通过免疫组织化学法检测各标本中Shh、Ptch-1、Gli-1阳性表达情况，采用逆转录聚合酶链反应（reverse transcription polymerase chain reaction，RT-PCR）法检测各标本中Shh、Ptch-1、Gli-1 mRNA表达情况，分析Shh、Ptch-1、Gli-1与前列腺癌临床分期、分化程度及血清前列腺特异性抗原（prostate specific antigen，PSA）含量的相关性。
结果 Shh、蛋白阳性信号主要表达于细胞质或细胞膜；Ptch-1蛋白阳性信号主要表达于细胞质中；Gli-1蛋白阳性信号主要表达于细胞核或细胞质中。前列腺癌组织标本Shh、Ptch-l、Gli-l阳性表达率均显著高于癌旁组织（P＜0.05）。前列腺癌组织标本中Shh与Ptch-1呈正相关（P＜0.05），Shh与Gli-1,Ptch-1 与Gli-1无相关性（P＞0.05）。不同临床分期、分化程度、血清PSA含量前列腺癌组织Shh、Ptch-1、Gli-1 mRNA表达量差异均有统计学意义（P＜0.05）。前列腺癌组织Shh、Ptch-1、Gli-1 mRNA表达情况与临床分期、分化程度、血清PSA含量均呈正相关（P＜0.05）。
结论前列腺癌组织中Shh、Ptch-1、Gli-1表达水平上调，其表达水平与前列腺癌临床分期及分化程度有关，提示Shh、Ptch-1、Gli-1与前列腺癌的发生与进展有密切关联，抑制Hh信号通路对前列腺癌的诊治具有重大意义。

关键词: 前列腺肿瘤, Sonic刺猬信号通路, 脑胶质瘤相关癌基因1

Abstract: Objective To investigate the expression and significance of Hedgehog(Hh) signaling pathway-related factors Sonic hedgehog(Shh), Patched-1(Ptch-1) and glioma-related oncogene homology-1(Gli-1) in prostate cancer.
Methods Sixty cases of prostate cancer and normal tissue specimens adjacent to prostate cancer were collected. The positive expressions of Shh, PTCH-1 and GLI-1 in each sample were detected by immunohistochemistry. The mRNA expression of Shh, Ptch-1 and Gli-1 in each specimen was detected by reverse transcription polymerase chain reaction(RT-PCR). The correlation between Shh, PTCH-1, GLI-1 and clinical stage, differentiation degree and serum prostate-specific antigen(PSA) level of prostate cancer was analyzed.
Results The positive signal of Shh protein was mainly expressed in cytoplasm or membrane, the positive signal of Ptch-1 protein was mainly expressed in cytoplasm, and the positive signal of Gli-1 protein was mainly expressed in the nucleus or cytoplasm. The positive expression rates of Shh, Ptch-L and Gli-L in prostate cancer tissues were significantly higher than those in adjacent tissues(P＜0.05). There was a positive correlation between Shh and Ptch-1 in prostate cancer tissue specimens(P＜0.05), but no correlation between Shh and Gli-1, as well as between Ptch-1 and Gli-1(P＞0.05). There were significant differences in mRNA expression levels of Shh, Ptch-1 and Gli-1 in prostate cancer tissues with different clinical stages, degrees of differentiation and serum PSA level(P＜0.05). The mRNA expressions of Shh, Ptch-1 and Gli-1 in prostate cancer tissues were positively correlated with clinical stage, degree of differentiation and serum PSA level(P＜0.05).
Conclusion The up-regulated expression levels of Shh, Ptch-1 and Gli-1 in prostate cancer tissues are correlated with the clinical stage and differentiation degree of prostate cancer, suggesting that Shh, PTCH-1 and GLI-1 are closely associated with the occurrence and development of prostate cancer. Therefore, inhibition of Hh signaling pathway is of great significance for the diagnosis and treatment of prostate cancer.

Key words: prostatic neoplasms, sonic hedgehog, glioma related oncogene homology-1

张航, 张建坤. Hh信号通路相关因子Shh、Ptch-1和Gli-1在前列腺癌中的表达及意义 [J]. 河北医科大学学报, 2022, 43(7): 781-786.

ZHANG Hang, ZHANG Jian-kun. Expression and significance of Hh signaling pathway-related factors Shh, Ptch-1 and Gli-1 in prostate cancer [J]. Journal of Hebei Medical University, 2022, 43(7): 781-786.

[1]	黄继伟, 李健斐, 王佳, 刘郭坤, 李建丽, 王玉芳. IVIM技术成像量化参数与前列腺癌病理特征的关系及联合诊断研究[J]. 河北医科大学学报, 2021, 42(9): 1078-1083.
[2]	张杰, 郭莹, 霍阿伟, 张舵. DCE-MRI、表观扩散系数对前列腺癌分级诊断的应用比较[J]. 河北医科大学学报, 2021, 42(4): 444-447,453.
[3]	张明, 齐进春. 2019版中国泌尿外科前列腺癌诊断治疗指南更新要点解读[J]. 河北医科大学学报, 2021, 42(10): 1117-1122,1154.
[4]	庞雅湘1，韩华2，殷玥1，马国华3，牛洪琳4*. 过表达miR-140-5p促进前列腺癌细胞凋亡抑制其增殖[J]. 河北医科大学学报, 2020, 41(1): 7-11.
[5]	李勇,赵新鸿,孟晓东,陈涛,刘健,邱建宏 *. 前列腺癌组织中 Glypican-3 蛋白的表达及意义[J]. 河北医科大学学报, 2016, 37(4): 397-399.
[6]	穆占增;马宁. 河北汉族人群 IL6基因多态性与前列腺癌风险的关联性研究[J]. , 2014, 35(8): 964-964.
[7]	陆健斐. 超声造影在前列腺癌诊治中的作用[J]. , 2013, 34(5): 615-615.
[8]	邱梅婷. PSA和ALP联合检测在早期前列腺癌骨转移诊断中的应用[J]. , 2012, 33(6): 728-728.
[9]	诸海燕;石优红;姚玲华;谢少琴. 电话随访指导康复训练治疗前列腺癌根治术后尿失禁的疗效观察[J]. , 2012, 33(3): 283-283.
[10]	邹咏心;耿紫娴;王佳;徐涛. 外周血循环肿瘤细胞检测在诊断转移性前列腺癌中的应用[J]. , 2009, 30(9): 971-971.
[11]	卢东;田建华;李铭;张爱利;赵志红;倪晓辰;洪声涛. 前列腺淋巴肉瘤1例[J]. , 2006, 27(1): 9-9.

Hh信号通路相关因子Shh、Ptch-1和Gli-1在前列腺癌中的表达及意义

Expression and significance of Hh signaling pathway-related factors Shh, Ptch-1 and Gli-1 in prostate cancer

HTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

本文评价