Wnt/βcatenin信号通路在甲状腺乳头状癌中的表达及意义

doi:10.3969/j.issn.10073205.2017.06.011

河北医科大学学报

Wnt/βcatenin信号通路在甲状腺乳头状癌中的表达及意义

河北医科大学第二医院甲状腺乳腺外科，河北石家庄 050000

出版日期:2017-06-25 发布日期:2017-06-22
作者简介:严丽(1977-)，女，河北石家庄人，河北医科大学第二医院副主任医师，医学博士，从事甲状腺疾病诊治研究。
基金资助:
河北省医学科学研究重点课题（20160122）

Expression and significance of Wnt/βcatenin signal transduction pathway in papillary thyroid carcinoma

Department of Thyroid and Breast Surgery, the Second Hospital of
Heber Medical University, Shijiazhuang 050000, China

Online:2017-06-25 Published:2017-06-22

摘要/Abstract

摘要： ［摘要］目的探讨Wnt/βcatenin通路中Wnt通路抑制因子（Wnt inhibitory factor1，WIF1）、β连环蛋白（βcatenin）和细胞周期蛋白（cyclin D1）在甲状腺乳头状癌（papillary thyroid cancer,PTC）中的表达及意义。方法选取PTC患者60例，留取癌灶组织和癌旁组织标本。检测WIF1、βcatenin和cyclin D1 mRNA和蛋白表达，并分析WIF1 mRNA与βcatenin和cyclin D1 mRNA的相关性。结果PTC组织WIF1 mRNA表达水平和蛋白表达阳性率均显著低于癌旁组织，βcatenin、cyclin D1 mRNA表达水平和蛋白表达阳性率均显著高于癌旁组织（P＜005）。PTC淋巴结转移组WIF1 mRNA表达水平和蛋白表达阳性率低于无淋巴结转移组，βcatenin、cyclin D1 mRNA表达水平和蛋白表达阳性率高于无淋巴结转移组（P＜005）。不同性别、年龄、肿瘤直径、病灶数目和是否包膜侵犯PTC中WIF1、βcatenin、cyclin D1 mRNA表达水平和蛋白表达阳性率差异无统计学意义（P＞005）。WIF1 mRNA与βcatenin和cyclin D1 mRNA表达呈负相关。结论WIF1基因在PTC中表达下调促进了βcatenin蛋白在细胞质中累积，导致靶基因cyclin D1过度激活。WIF1下调和（或）βcatenin、cyclin D1上调在PTC的发生和转移中发挥促进作用。

关键词: 甲状腺肿瘤, Wnt信号通路, &beta, 连环素

Abstract: Abstract］ Objective To study expression and significance of Wnt inhibitory factor1(WIF1), βcatenin and chclin D1 in Wnt/βcatenin signal transduction pathway in papillary thyroid cancer（PTC）. Methods Sixty cases of PTC were selected and the specimens of cancerous foci and adjacent tissues were taken. The expression of WIF1, βcatenin and cyclin D1 mRNA and protein were detected, and the correlation between WIF1 mRNA and βcatenin, cyclin D1 mRNA was analyzed. ResultsThe expression levels of WIF1 mRNA and positive rates of WIF1 protein expression in PTC tissues were significantly lower than those in paracancerous tissues(P＜005), while expression levels of βcatenin, cyclin D1 mRNA and protein in PTC tissues were on the contrary(P＜005). And the expression levels of WIF1 mRNA and protein in PTC lymph node metastasis group was lower than those without lymph node metastasis, but the expression levels of βcatenin, cyclin D1 mRNA and protein in PTC lymph node metastasis group were on the contrary(P＜005). There were no statistically significant differences in the expression levels of WIF1, βcatenin, cyclin D1 mRNA and positive rates of protein in PTC tissues in different sex, age, tumor diameter, number of lesions and whether or not the capsule was invaded(P＞005). Expression levels of βcatenin, cyclin D1 mRNA were negatively correlated with those of WIF1 mRNA(P＜005). ConclusionThe down regulation mechanism of WIF1 gene in PTC tissues promoted the accumulation of βcatenin protein in the cytoplasm, which resulted in over activation of target gene cyclin D1. The WIF1 down regulation or βcatenin and cyclin D1 up regulation mechanism may play an auxoaction role in the occurrence and metastasis in PTC.

Key words: thyroid neoplasms, Wnt signaling pathway, beta catenin

严丽，李清怀，冀宏，郝芳. Wnt/βcatenin信号通路在甲状腺乳头状癌中的表达及意义[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2017.06.011.

YAN Li, LI Qinghuai, JI Hong, HAO Fang. Expression and significance of Wnt/βcatenin signal transduction pathway in papillary thyroid carcinoma[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2017.06.011.

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Wnt/βcatenin信号通路在甲状腺乳头状癌中的表达及意义

Expression and significance of Wnt/βcatenin signal transduction pathway in papillary thyroid carcinoma

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