河北医科大学学报

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丰富环境通过ERK1/2信号通路促进脑缺血后神经功能恢复

  

  1. 1.湖北医药学院附属东风医院急救医学部，湖北 十堰 442008；2.国药东风总医院综合病区，湖北 十堰 442700
  • 出版日期:2019-08-25 发布日期:2019-08-14
  • 作者简介:饶楚炳(1975-)，男，湖北郧西人，湖北医药学院附属东风医院主治医师，从事急诊医学研究。

Enriched environment promotes poststroke neurological function recovery through regulation ERK1/2 pathway#br#

  1. 1.Department of Emergency Medicine， the Dongfeng Hospital Affiliated to Hubei University of Medicine，
    Hubei Province, Shiyan 442008, China; 2.Department of General Ward， Sinopharm Dongfeng
    General Hospital， Hubei Province, Shiyan  442700, China
  • Online:2019-08-25 Published:2019-08-14

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摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗观察丰富环境（enriched environment,EE）对脑缺血后神经功能恢复的影响并探讨作用机制。
〖HTH〗方法〖HTSS〗〖KG*2〗采用线栓法制备大鼠大脑中动脉栓塞（middle cerebral artery occlusion，MCAO）模型，按照处理方式分为脑缺血后EE饲养组（Ischemia & EE组）、脑缺血后标准饲养组（Ischemia & SC组）、假手术后标准饲养组（Sham & SC组），分别于术前及术后3 d、5 d、7 d、14 d、21 d应用跑梯实验、前肢放置实验、前肢不对称实验、前爪伸曲实验检测大鼠行为学康复情况。MCAO后21 d，透射电镜观察星形胶质细胞形态学改变，Western blotting检测脑缺血组织tERK1/2、pERK1/2蛋白表达。
〖HTH〗结果〖HTSS〗〖KG*2〗Ischemia & EE组和Ischemia & SC组术后3 d跑梯实验、前肢放置实验、前肢不对称实验和前爪伸曲实验错误评分明显上升，术后5～21 d逐渐下降，但Ischemia & SC组术后21 d下降程度不如Ischemia & EE组大，Sham & SC组在以上实验的各时点有上下波动，但变化不大，各组跑梯实验、前肢放置实验、前肢不对称实验和前爪伸曲实验错误评分在组间、时点间以及组间·时点间交互作用差异均有统计学意义（P＜005）。Ischemia & SC组pERK1/2表达量明显高于Ischemia & EE组，Sham & SC组明显低于Ischemia & SC组，差异均有统计学意义（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗EE可能通过抑制ERK1/2信号通路促进脑缺血后大鼠神经功能的恢复。

关键词: 缺氧缺血, 脑, 丰富环境, ERK1/2信号通路

Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the effects and the mechanism of enriched environment on the neurological function recovery after stroke.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Adult rats were subjected to ischemia induced by middle cerebral artery occlusion(MCAO) followed by reperfusion, rats were assigned Ischemia & EE group, Ischemia & SC group and Sham & SC group. Ladder rung walking test, forelimb placing test, limbuse asymmetry test and paw reaching test were used to assess the recovery of rat behavior before surgey and on postoperative days 3, 5, 7, 14 and 21, respectively. Expressions of phosphorylated ERK1/2(pERK1/2) and total ERK1/2(tERK1/2) were examined by western bloting, and electron microscopy was used to evaluate the astrocytes morphology surround in the perivascular 21 days after MCAO.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓On the third day after MCAO, the scores of ladder rung walking test, forelimb placing test, limbuse asymmetry test and paw reaching test in Ischemia & EE and Ischemia & SC group were significantly increased, and then, those test scores were decreased from 5 to 21 days after surgery. However, the rats in the Ischemia & EE showed lower test scores compared to the rats in the Ischemia & SC group. The test scores mentioned above were fluctuated on postoperative days 3, 5, 7, 14 and 21, however, those changes were no significant difference. The differences of ladder rung walking test, forelimb placing test, limbuse asymmetry test and paw reaching test score were statistically significant for the effect of time, group and group by time interaction(P＜005). Ischemia & SC group showd increased pERK1/2 level compared to Ischemia & EE group, however, the level of pERK1/2 among Sham & SC group is lower than that among Ischemia & SC group(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Enriched environment could promote rehabilitation of rat after ischemia via inhibition of ERK1/2 signal pathway.

Key words: hypoxiaischemia, brain, enriched environment; , ERK1/2 signal pathway

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