3种结核分枝杆菌耐药检测方法的对比研究#br#

doi:10.3969/j.issn.1007-3205.2019.11.016

河北医科大学学报

3种结核分枝杆菌耐药检测方法的对比研究#br#

1.河北省胸科医院检验科，河北石家庄 050041；2.河北省胸科医院超声科，河北石家庄 050041；
3.河北医科大学第四医院检验科，河北石家庄 050011；4.河北省唐县人民医院影像科，河北唐县 072350

出版日期:2019-11-25 发布日期:2019-11-21
作者简介:薛建昌（1977-），男，河北唐县人，河北省胸科医院主管检验师，医学硕士，从事临床检验学研究。
基金资助:
河北省医学科学研究重点课题（20170424）

Comparative study on three methods for detecting drug resistance of Mycobacterium tuberculosis

1.Department of Laboratory, Hebei Chest Hospital, Shijiazhuang 050041，China； 2.Department of
Ultrasound, Hebei Chest Hospital, Shijiazhuang 050041, China； 3.Department of Laboratory, the Fourth
Hospital of Hebei Medical University, Shijiazhuang 050011, China； 4.Department of Medical Imaging,
the People′s Hospital of Tangxian, Hebei Province， Tanxian 072350, China

Online:2019-11-25 Published:2019-11-21

摘要/Abstract

摘要： ［摘要］
〖HTH〗目的〖HTSS〗〖KG*2〗比较BACTEC MGIT 960药敏法（BD960）、噬菌体生物扩增法（pha B）、荧光定量PCR探针熔解曲线法（PCR法）3种方法检测结核分枝杆菌耐药的临床效能，并评估其应用价值。
〖HTH〗方法〖HTSS〗〖KG*2〗收集结核分枝杆菌阳性的临床分离株，采用BD960法、pha B法和PCR法检测抗结核一线药物耐药情况，比较3种方法的检测结果。
〖HTH〗结果〖HTSS〗〖KG*2〗 pha B法和PCR法平均报告时间明显短于BD960法，PCR法平均报告时间明显短于pha B法，差异有统计学意义（P＜0.05）。3种方法对80株临床分离株进行链霉素（streptomycin，SM）、异烟肼（isoniazid，INH）、利福平（rifampin，RFP）和乙胺丁醇（ethambutol，EMB）耐药检测，耐药检出结果差异均无统计学意义（P＞0.05）。以BD960法检测结果作为对照，pha B法对SM、INH和RFP耐药检测结果均为高度一致，对EMB检测结果为中度一致；PCR法对INH和RFP耐药检测结果高度一致，对SM和EMB检测结果为中度一致。
〖HTH〗结论〖HTSS〗〖KG*2〗应用PCR法和pha B能快速筛查结核分枝杆菌对一线抗结核药物的耐药情况。

关键词: 结核分枝杆菌, 抗药性, 对比研究

Abstract: ［Abstract］Objective〖HTSS〗To compare the clinical efficacy of BACTEC MGIT 960 drug sensitivity method(BD960), bacteriophage biological amplification(pha B) and fluorescence quantitative PCR probe melting curve method in detecting drug resistance of Mycobacterium tuberculosis, and to evaluate its application value.
〖HTH〗〖WTHZ〗Methods〖HTSS〗The clinical isolates were identified as the positive clinical isolates of Mycobacterium tuberculosis. The results of the three methods were compared and analyzed by using the BD960, pha B and PCR at the same time.
〖HTH〗〖WTHZ〗Results〖HTSS〗The average reporting time of pha B method and PCR method was significantly shorter than that of BD960 method, and PCR method was significantly shorter than that of pha B method, and the difference was statistically significant (P＜0.05). At the same time, the resistance of 80 clinical isolates were detected by streptomycin, isoniazid, rifampicin and ethambutol, and there was no significant difference between the three methods(P＞0.05). BD960 method was used as the control, the resistance of streptomycin(SM), isoniazid(INH) and rifampin(RFP) by phaB was highly consistent, and that of ethambutol（EMB） was moderate. The results of PCR resistance to INH and RFP were highly consistent and that of SM and EMB were moderate.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The resistance of Mycobacterium tuberculosis to first-line antituberculosis drugs can be quickly screened by fluorescence quantitative PCR probe melting curve and bacteriophage biological amplification.

Key words: mycobacterium tuberculosis, drug resistance, comparative study

薛建昌1，吴海峰1，郑浩2*，张建武1，张金艳3，王国英4. 3种结核分枝杆菌耐药检测方法的对比研究#br#[J]. 河北医科大学学报, doi: 10.3969/j.issn.1007-3205.2019.11.016.

XUE Jian-chang1,WU Hai-feng1， ZHENG Hao2*, ZHANG Jian-wu1, ZHANG Jin-yan3, WANG Guo-ying4. Comparative study on three methods for detecting drug resistance of Mycobacterium tuberculosis[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.1007-3205.2019.11.016.

[1]	杜若昕, 郭欣燕（综述）, 张伟, 芦希艳（审校）. 肝细胞癌中索拉非尼耐药性机制的研究进展[J]. 河北医科大学学报, 2022, 43(9): 1091-1095.
[2]	乔宏伟, 房岩（综述）, 方子源, 赵占娟（审校）. PACT的抗菌作用及其机制的研究进展[J]. 河北医科大学学报, 2021, 42(9): 1113-1116,封三.
[3]	侯军良, 冯爱东, 刘腾飞, 许怡, 张海从, 杨莉. 河北省石家庄地区HBV基因分型及耐药突变分析[J]. 河北医科大学学报, 2021, 42(7): 851-855.
[4]	贾晨光，刘丰胜，姚晓伟，高建国，白洪忠*. 骨关节结核不同类型标本进行Xpert MTB/RIF技术检测的研究[J]. 河北医科大学学报, 2020, 41(6): 650-653,679.
[5]	薛建昌1，邸红芹2，吴海峰1，郑浩3*，郑立恒1，梁冰锋4. 3种常规结核分枝杆菌及耐药突变检测方法的对比研究[J]. 河北医科大学学报, 2020, 41(6): 715-719.
[6]	穆卫红，陈文琪，彭园园，张静*. miR-141-3p表达与卵巢癌细胞顺铂化疗敏感性的相关性研究[J]. 河北医科大学学报, 2020, 41(2): 167-171.
[7]	刘子腾，吴瑞锋，杨炜琦，张雪莲，周伯良. 杂交冠状动脉血运重建术在冠心病治疗中的应用价值[J]. 河北医科大学学报, 2019, 40(6): 667-670.
[8]	刘凯军1，李茂恒1，王文涛2，魏〓娉3. 胃癌组织中TPX2与肿瘤细胞铂类药物耐药的关系及意义[J]. 河北医科大学学报, 2019, 40(6): 671-674,678.
[9]	马亚贞1，李索林1*，薛文勇2，齐进春2，王文博1，杜〓蕾2. 2种腹腔镜睾丸固定术治疗小儿隐睾效果对比观察#br#[J]. 河北医科大学学报, 2019, 40(4): 451-455.
[10]	李志荣1，2，赵建宏1，2，李继红1，范士英1，孙〓倩1，时东彦1*. 2016—2017年河北省二级医院血液标本细菌分布及耐药性分析[J]. 河北医科大学学报, 2019, 40(3): 342-346.
[11]	吴〓菲，陈惠兰，王伟伟，冯向春. 151例足月与早产新生儿化脓性脑膜炎的对比分析[J]. 河北医科大学学报, 2018, 38(7): 837-840.
[12]	王〓力1，王天轶2，温晓华1*，刘艳如1，胡兴茂3，高〓冕1. 高血压慢病患者的慢病团队管理效果研究#br#[J]. 河北医科大学学报, 2018, 38(6): 645-648,652.
[13]	李炳南，胡桂君，郑〓海，武〓容，蔺振军，李奎蒙. 前路与后路手术治疗胸腰椎爆裂性骨折的临床效果#br#[J]. 河北医科大学学报, 2018, 38(6): 672-676.
[14]	刘建宁1，王〓伟2，贾〓蓓2*. 重组人酸性成纤维细胞生长因子治疗开放伤的随机临床研究[J]. 河北医科大学学报, 2018, 38(6): 714-716.
[15]	田景辉（综述），吴利先*（审校）. HIV/AIDS合并NTM肺病在临床诊疗方面的研究进展[J]. 河北医科大学学报, 2018, 38(4): 476-480.

3种结核分枝杆菌耐药检测方法的对比研究#br#

Comparative study on three methods for detecting drug resistance of Mycobacterium tuberculosis

HTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价