miR-130b-3p在肾癌中表达及PI3K/AKT途径参与的机制研究

doi:10.3969/j.issn.1007-3205.2023.12.004

河北医科大学学报 ›› 2023, Vol. 44 ›› Issue (12): 1386-1392.doi: 10.3969/j.issn.1007-3205.2023.12.004

miR-130b-3p在肾癌中表达及PI3K/AKT途径参与的机制研究

河北工程大学附属医院泌尿外二科，河北邯郸 056002

出版日期:2024-01-02 发布日期:2024-01-02
作者简介:谭超（1992-），男，河北邯郸人，河北工程大学附属医院主治医师，医学硕士，从事泌尿系肿瘤疾病诊治研究。
基金资助:
邯郸市科学技术与研究发展项目(19422083011-13)

Expression of miR-130b-3p in renal cell carcinoma and the mechanism of PI3K/AKT pathway involvement

The Second Department of Urology, the Affiliated Hospital of Hebei University of Technology, Handan 056002, China

Online:2024-01-02 Published:2024-01-02

摘要/Abstract

摘要： 目的探究miR-130b-3p调节PI3K/AKT途径对肾癌细胞生物学行为的影响及作用机制。
方法临床收集肾癌患者78例，检测肿瘤组织和癌旁组织中miR-130b-3p和PTEN蛋白水平，分析miR-130b-3p与肾癌病理特征的关系。通过相关性分析探究肾癌组织中miR-130b-3p与PTEN蛋白的相关性。786-O细胞分为NC、miR-130b-3p、PTEN和miR-130b-3p+PTEN组，分别转染NC、miR-130b-3p mimic和/或pcDNA 3.1 PTEN来过表达miR-130b-3p和/或PTEN。分别检测各组细胞活力、凋亡率、侵袭能力以及PI3K/AKT通路水平。
结果肾癌组织中miR-130b-3p水平显著高于癌旁组织（P＜0.05）。miR-130b-3p表达水平与性别、年龄、肿瘤直径无关，高水平的miR-130b-3p与高临床分期和淋巴转移有关（P＜0.05）。miR-130b-3p与PTEN靶向结合（P＜0.05）。与NC组比较，miR-130b-3p组的PTEN蛋白和凋亡率显著降低，增殖活力、侵袭能力和PI3K/AKT通路显著升高（P＜0.05）；PTEN组的PTEN蛋白和凋亡率显著升高，增殖活力、侵袭能力和PI3K/AKT通路显著抑制（P＜0.05）。miR-130b-3p+PTEN组的PTEN蛋白和凋亡率显著高于miR-130b-3p组且显著低于PTEN组，而增殖活力、侵袭能力和PI3K/AKT通路显著低于miR-130b-3p组且显著高于PTEN组（P＜0.05）。
结论 miR-130b-3p在肾癌组织中上调，高水平的miR-130b-3p与肾癌患者的高临床分期和转移有关，miR-130b-3p可调控PI3K/AKT途径，抑制PTEN蛋白表达，促进肾癌细胞的侵袭并抑制凋亡。

关键词: 肾肿瘤, 微小RNA, miR-130b-3p

Abstract: Objective To explore the effect and mechanism of miR-130b-3p regulating PI3K/AKT pathway on the biological behavior of renal cancer cells.
Methods A total of 78 renal cancer patients were clinically selected. The protein levels of miR-130b-3p and PTEN in tumor tissues and adjacent tissues were detected, and the relationship between miR-130b-3p and pathological features of renal cancer was analyzed. The correlation between miR-130b-3p and PTEN protein in renal cancer tissue was explored by correlation analysis. 786-O cells were divided into negative control (NC) group, miR-130b-3p, PTEN and miR-130b-3p+PTEN groups. NC, miR-130b-3p mimic and/or pcDNA 3.1 PTEN were transfected to overexpress miR-130b-3p and/or PTEN, respectively. The cell viability, apoptosis rate, invasion ability and PI3K/AKT pathway level in each group were detected respectively.
Results The level of miR-130b-3p in renal cancer tissues was significantly higher than that in adjacent tissues (P＜0.05). The expression level of miR-130b-3p was independent of gender, age and tumor diameter. High level of miR-130b-3p was associated with high clinical stage and lymphatic metastasis (P＜0.05), with miR-130b-3p targeted binding to PTEN (P＜0.05). Compared with the NC group, the PTEN protein and apoptosis rate of the miR-130b-3p group were significantly decreased, and the proliferation activity, invasion ability and PI3K/AKT pathway were significantly increased (P＜0.05). In the PTEN group, the PTEN protein and apoptosis rate were significantly increased, while the proliferation activity, invasion ability and PI3K/AKT pathway were significantly inhibited (P＜0.05). The PTEN protein and apoptosis rate of the miR-130b-3p+PTEN group were significantly higher than those of the miR-130b-3p group and significantly lower than those of the PTEN group, while the proliferation activity, invasion ability and PI3K/AKT pathway were significantly lower than those of the miR-130b-3p group, and significantly higher than those of the PTEN group (P＜0.05).
Conclusion miR-130b-3p is upregulated in renal cancer tissues, and high levels of miR-130b-3p are associated with high clinical stage and metastasis in renal cancer patients. miR-130b-3p can regulate PI3K/AKT pathway, inhibit the expression of PTEN protein, promote the invasion of renal cell carcinoma cells and inhibit apoptosis.

Key words: kidney neoplasms, microRNA, miR-130b-3p

谭超, 陈海滨, 李科技, 赵建军 . miR-130b-3p在肾癌中表达及PI3K/AKT途径参与的机制研究 [J]. 河北医科大学学报, 2023, 44(12): 1386-1392.

TAN Chao, CHEN Hai-bin, LI Ke-ji, ZHAO Jian-jun. Expression of miR-130b-3p in renal cell carcinoma and the mechanism of PI3K/AKT pathway involvement [J]. Journal of Hebei Medical University, 2023, 44(12): 1386-1392.

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[13]	苏世强1，刘丽哲2*，王香芝1，李瑞玲1，赵敬霞1,庞书舰1. 术中冰冻切片对复杂性肾囊性病变的临床诊断价值[J]. 河北医科大学学报, 2017, 37(1): 86-88.
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[15]	张斌;张威;曹春蕊. 32例囊性肾癌的CT诊断及误诊分析[J]. , 2014, 35(7): 856-856.

miR-130b-3p在肾癌中表达及PI3K/AKT途径参与的机制研究

Expression of miR-130b-3p in renal cell carcinoma and the mechanism of PI3K/AKT pathway involvement

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