河北医科大学学报 ›› 2023, Vol. 44 ›› Issue (6): 654-659.doi: 10.3969/j.issn.1007-3205.2023.06.007

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外周血miR-92b-5p与miR-148b-3p在2型糖尿病进展至糖尿病肾病中的表达及交互作用

  

  1. 河北省石家庄市第二医院内分泌科，河北 石家庄 050000
  • 出版日期:2023-06-25 发布日期:2023-06-29
  • 作者简介:邢玉微（1983-），女，河北保定人，河北省石家庄市第二医院副主任医师，医学硕士，从事糖尿病及其并发症的诊治研究。
  • 基金资助:
    石家庄市科学技术研究与发展计划（201201193）

Expression and interaction of peripheral blood miR-92b-5p and miR-148b-3p in the progression of type 2 diabetes to diabetic nephropathy

  1. Department of Endocrinology, the Second Hospital of Shijiazhuang City, Hebei Province, 
    Shijiazhaung 050000， China
  • Online:2023-06-25 Published:2023-06-29

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摘要： 目的 探究2型糖尿病（type 2 diabetes，T2DM）进展至糖尿病肾病（diabetic nephropathy，DN）过程中血清微小RNA（microRNA，miR）-92b-5p、miR-148b-3p水平变化和作用。
方法 选取我院T2DM患者400例开展前瞻性研究，其中200例非DN患者作为T2DM组，200例DN患者作为DN组。比较两组临床资料、血清miR-92b-5p、miR-148b-3p水平，并比较DN组不同分期患者血清miR-92b-5p、miR-148b-3p水平，分析DN组血清miR-92b-5p、miR-148b-3p水平与临床分期的相关性，并分析T2DM进展至DN过程中血清miR-92b-5p与miR-148b-3p的交互作用，及血清miR-92b-5p、miR-148b-3p水平预测DN的价值。
结果 DN组血肌酐（serum creatinine，SCr）、血清miR-148b-3p水平高于T2DM组，估算肾小球滤过率（estimated glomerular filtration rate，eGFR）及血清miR-92b-5p水平低于T2DM组（P＜0.05）；DN组临床期患者血清miR-148b-3p水平高于早期患者，血清miR-92b-5p水平低于早期患者（P＜0.05）；DN组血清miR-92b-5p水平与临床分期呈负相关，血清miR-148b-3p水平与临床分期呈正相关（P＜0.05）；在T2DM进展至DN过程中，miR-92b-5p低表达与miR-148b-3p高表达呈次相乘模型的正向交互作用（P＜0.05）；miR-92b-5p、miR-148b-3p预测T2DM进展至DN的曲线下面积（area under the curve，AUC）分别为0.840、0.812，二者联合预测的AUC最大，为0.911。
结论 DN患者血清miR-92b-5p水平明显降低，miR-148b-3p水平明显升高，且与DN临床分期密切相关，miR-92b-5p低表达、miR-148b-3p高表达共同促进T2DM进展至DN过程。


关键词: 糖尿病, 2型, 糖尿病肾病, 微小RNA-92b-5p

Abstract: Objective To investigate the changes in serum microRNA (miR)-92b-5p and miR-148b-3p levels and their role in the progression of type 2 diabetes mellitus (T2DM) to diabetic nephropathy (DN). 
Methods A prospective study was conducted on 400 T2DM patients in our hospital, with 200 non-DN patients as the T2DM group and 200 DN patients as the DN group. The clinical data, serum miR-92b-5p and miR-148b-3p levels of the two groups were compared, and the serum miR-92b-5p and miR-148b-3p levels of patients with different stages in the DN group were compared. The correlation between serum miR-92b-5p and miR-148b-3p levels and clinical stages in the DN group, the interaction between serum miR-92b-5p and miR-148b-3p in the progression of T2DM to DN, and the value of serum miR-92b-5p and miR-148b-3p levels in predicting DN were analyzed. 
Results In the DN group, serum creatinine (SCr) and serum miR-148b-3p levels were higher than those in the T2DM group, and the estimated glomerular filtration rate (eGFR) and serum miR-92b-5p levels were lower than those in the T2DM group (P＜0.05). Serum miR-148b-3p levels in the DN group were higher, and serum miR-92b-5p levels were lower than those in the early stage (P＜0.05). Serum miR-92b-5p levels in the DN group were negatively correlated with clinical stage and serum miR-148b-3p levels were positively correlated with clinical stage (P＜0.05). The positive interaction between low expression of miR-92b-5p and high expression of miR-148b-3p in the progression of T2DM to DN was a submultiplicative model (P＜0.05). The area under the curve (AUC) of miR-92b-5p and miR-148b-3p in predicting T2DM progression to DN was 0.840 and 0.812, respectively, and the combined prediction of the two had the largest AUC (0.911). 
Conclusion Serum miR-92b-5p levels are significantly lower and miR-148b-3p levels are significantly higher in DN patients, which are closely related to the clinical stage of DN. Low expression of miR-92b-5p and high expression of miR-148b-3p contribut to the progression of T2DM to DN. 


Key words: diabetes mellitus, type 2, diabetic nephropathy, microRNA-92b-5p

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