环状RNA sirt1对胃癌细胞焦亡和肿瘤血管生成的影响研究

doi:10.3969/j.issn.1007-3205.2025.06.004

河北医科大学学报 ›› 2025, Vol. 46 ›› Issue (6): 642-648.doi: 10.3969/j.issn.1007-3205.2025.06.004

环状RNA sirt1对胃癌细胞焦亡和肿瘤血管生成的影响研究

1.河北省唐山市人民医院消化中心，河北唐山 063000；2.河北省唐山市人民医院胃肠外一科，
河北唐山 063000；3.河北医科大学第一医院胃肠病诊疗四科，河北石家庄050031

出版日期:2025-06-25 发布日期:2025-07-04
作者简介:何蕴灏（1991-），女，满族，河北遵化人，河北省唐山市人民医院主治医师，医学硕士，从事消化系统肿瘤疾病诊治研究。
基金资助:
北省政府资助临床医学优秀人才培养项目（ZF2025045）；唐山市人才资助项目（A202203027）

Effect of circular RNA sirt1 on pyroptosis and tumor angiogenesis in gastric cancer cells

1.Digestive Center, Tangshan People′s Hospital, Hebei Province, Tangshan 063000, China;
2.Department of Gastroenterology, Tangshan People′s Hospital, Hebei Province, Tangshan
063000, China; 3.The Fourth Department of Gastroenterology, the First Hospital of
Hebei Medical University, Shijiazhuang 050031, China

Online:2025-06-25 Published:2025-07-04

摘要/Abstract

摘要： 目的探究环状RNA sirt1（circular RNAsirt1,circ-sirt1）的表达对胃癌细胞焦亡和血管生成的影响。
方法收集河北省唐山市人民医院和河北医科大学第一医院胃癌患者30例的病理组织样本，检测circ-sirt1和细胞焦亡相关蛋白表达水平。培养胃癌细胞系AGS（Adenocarcinoma Gastric Stomach）、HGC27（Human Gastric Carcinoma-27）、NUGC3（Nagoya University-Gastric Cancer-3）、SNU1（Seoul National University-1）、SNU601（Seoul National University-601）和正常胃上皮细胞GES-1（Gastric Mucosal Epithelial Cell Line-1），检测circ-sirt1、细胞焦亡相关蛋白表达水平。对HGC27细胞进行circ-sirt1敲低或过表达处理，检测细胞增殖活性、血管生成能力、细胞焦亡相关蛋白水平变化情况。
结果与癌旁组织、GES-1细胞比较，胃癌组织、胃癌细胞中cicr-sirt1和细胞焦亡相关蛋白表达水平均降低。敲低circ-sirt1后，HGC27细胞增殖活性、血管生成能力增强，细胞焦亡水平降低；过表达circ-sirt1后，HGC27细胞增殖活性、血管生成能力减弱，细胞焦亡水平升高。
结论circ-sirt1在胃癌组织和胃癌细胞系中均表达下调，过表达circ-sirt1能够升高胃癌细胞焦亡水平，并抑制肿瘤血管生成。

关键词: 胃肿瘤, 环状RNA sirt1, 细胞焦亡, 血管生成

Abstract: Objective To investigate the effect of circular RNA sirt1 (circ-sirt1) expression on pyroptosis and angiogenesis in gastric cancer cells.
Methods Pathological tissue samples were collected from 30 gastric cancer patients from Tangshan People′s Hospital and the First Hospital of Hebei Medical University, and the expression levels of circ-sirt1 and pyroptosis-related proteins were detected. Gastric cancer cell lines adenocarcinoma gastric stomach (AGS), human gastric carcinoma-27 (HGC27), Nagoya University-Gastric Cancer-3 (NUGC3), Seoul National University-1 (SNU1), Seoul National University-601 (SNU601) and normal gastric mucosal epithelial cell line-1 (GES-1) were cultured, and the expression levels of circ-sirt1 and pyroptosis-related proteins were detected. Knockdown or overexpression of circ-sirt1 was performed on HGC27 cells, and the changes of cell proliferation activity, angiogenesis ability and pyroptosis-related protein levels were detected.
Results Compared with para-cancerous tissue and GES-1 cells, the expression levels of circ-sirt1 and pyroptosis-related proteins were decreased in gastric cancer tissue and gastric cancer cells. After knocking down circ-sirt1, the proliferation activity and angiogenesis ability of HGC27 cells were enhanced, while the level of pyroptosis was decreased. After overexpressing circ-sirt1, the proliferation activity and angiogenesis ability of HGC27 cells were weakened, while the level of pyroptosis was increased.
Conclusion circ-sirt1 is down-regulated in gastric cancer tissue and gastric cancer cell lines, and overexpression of circ-sirt1 can increase the level of pyroptosis in gastric cancer cells and inhibit tumor angiogenesis.

Key words: stomach neoplasms, circular RNA sirt1, pyroptosis, angiogenesis

何蕴灏1, 李青科2, 张云丽1, 张建蕊1, 赵媛1, 韩双双3. 环状RNA sirt1对胃癌细胞焦亡和肿瘤血管生成的影响研究[J]. 河北医科大学学报, 2025, 46(6): 642-648.

HE Yun-hao1, LI Qing-ke2, ZHANG Yun-li1, ZHANG Jian-rui1, ZHAO Yuan1, HAN Shuang-shuang3. Effect of circular RNA sirt1 on pyroptosis and tumor angiogenesis in gastric cancer cells[J]. Journal of Hebei Medical University, 2025, 46(6): 642-648.

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环状RNA sirt1对胃癌细胞焦亡和肿瘤血管生成的影响研究

Effect of circular RNA sirt1 on pyroptosis and tumor angiogenesis in gastric cancer cells

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