Abstract: ［Abstract］〓Objective〖HTSS〗〓To discuss the effect of miR19a3p on the proliferation of human gastric cancer cell via targeting PTEN, which provided new ideas for the prevention and treatment of gastric cancer.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Gastric carcinoma tissue specimens and matched adjacent tissues were obtained from twenty patients who were identified as aggressive gastric adenocarcinoma. Realtime PCR was used to analyze the levels of miR19a3p in gastric carcinoma tissue specimens and matched adjacent tissues. miR19a3p mimic and miR19a3p inhibitor were transfected into MKN28 cells. Realtime PCR was used to analyze the level of miR19a3p. And MTT assay was used to determine the proliferation of MKN28 cell. miR19a3p mimic and miR19a3p inhibitor were transfected into MKN28 cells. Western blot was used to measure the protein level of PTEN. miR19a3p inhibitor and siRNAPTEN were transfected into MKN28 cells. Western blot was used to measure the protein level of PTEN. And MTT assay was used to determine the proliferation of MKN28 cell.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with adjacent tissues, miR19a3p expression was increased in gastric cancer tissues(P＜005). Compared with negative mimic control group, transfection with miR19a3p mimic increased miR19a3p expression(P＜005). Compared with negative inhibitor control group, transfection with miR19a3p inhibitor decreased miR19a3p expression(P＜005). The level of PTEN mRNA did not significantly change in MKN28 cells transfected with miR19a3p mimic(P＞005) or miR19a3p inhibitor(P＞005). The proliferation of MKN28 cells was inhibited in MKN28 cells transfected with miR19a3p inhibitor(P＜005) and was promoted in MKN28 cells transfected with siPTEN(P＜005). Compared with siPTEN group, the proliferation of MKN28 cells was decreased in MKN28 cells cotransfected with siPTEN and miR19a3p inhibitor(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓miR19a3p regulated the proliferation of human gastric cancer cells via targeting PTEN.

Key words: stomach neoplasms, microRNAS, cell proliferation