Journal of Hebei Medical University ›› 2023, Vol. 44 ›› Issue (12): 1442-1447.doi: 10.3969/j.issn.1007-3205.2023.12.013

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Study on the correlation between total bile acid in gastric juice, Helicobacter pylori infection and gastric cancer

  

  1. 1.The First Department of Gastroenterology, Shijiazhuang People′s Hospital, Hebei Province, 
    Shijiazhuang 050000, China; 2.The Fifth Department of Gastroenterology, Shijiazhuang People′s 
    Hospital, Hebei Province, Shijiazhuang 050000, China; 3.The Second Department of 
    Gastroenterology, Shijiazhuang People′s Hospital, Hebei Province, 
    Shijiazhuang 050000, China

  • Online:2024-01-02 Published:2024-01-02

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Abstract: Objective To investigate the relationship of total bile acid (TBA) in gastric juice and helicobacter pylori (Hp) infection with gastric cancer. 
Methods Sixty patients diagnosed with gastric cancer under gastroscopy were selected as the research group. Adenocarcinoma was diagnosed by pathological examination. During the same period, 60 people for physical examination and with chronic non-atrophic gastritis (CNG) indicated by gastroscope were selected as the control group. Pathology showed no gland atrophy, dysplasia, or intestinal metaplasia. Gastric juice was extracted from the patients to detect the concentration of TBA, the expression of caudal type homeobox 2 (CDX2), mucin 2 (MUC2) and mucin 5AC (MUC5AC) in the tissue specimens were detected by immunohistochemical staining, and rapid urease test and histological modified Giemsa staining were used to detect Hp. 
Results The TBA concentration in the research group was higher than that in the control group, and the Hp positive infection rate was higher than that in the control group (P＜0.05). There were 32 patients with CDX2 positive expression in the research group, accounting for 53.33%, 33 patients with MUC2 positive expression, accounting for 55.00%, and 25 patients with MUC5AC positive expression, accounting for 41.67%. There was no positive expression of CDX2 and MUC2 in the control group, while MUC5AC was positive in 52 cases, accounting for 86.67%. The positive expression rates of CDX2 and MUC2 in the research group were higher than those in the control group, while the positive expression rate of MUC5AC was lower than that in the control group (P＜0.05). The positive expression rates of CDX2 and MUC2 in the Hp positive group were higher than those in the Hp negative group, while the positive expression rate of MUC5AC was lower than that in the Hp negative group (P＜0.05). Positive Hp and gastric TBA concentration ≥ 300 μmol/L were risk factors for CDX2 and MUC2 positive expression (P＜0.05), and protective factors for MUC5AC positive expression (P＜0.05). 
Conclusion TBA concentration in gastric fluid ≥ 300 μmol/L and Hp positivity are risk factors for CDX2 and MUC2 positive expression, and are protective factors for MUC5AC positive expression. TBA in gastric fluid and Hp may affect the occurrence of gastric cancer by affecting the expression of CDX2, MUC2, and MUC5AC. 


Key words: stomach neoplasms, cholic acids, Helicobacter pylori infection