Journal of Hebei Medical University ›› 2022, Vol. 43 ›› Issue (3): 258-264.doi: 10.3969/j.issn.1007-3205.2022.03.003

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Microarray identification of genes and microRNA analysis related to idiopathic pulmonary fibrosis

  

  1. 1.Department of Respiratory and Critical Care Medicine, Provincial Hospital of Shandong First Medical

    University, Jinan 250000, China; 2.Department of General Medicine, Jinan People′s Hospital of

    Jinan City, Shandong Province, Jinan 271100, China; 3.Department of Public Course Teaching, Cangzhou Medical College, Hebei Province, Cangzhou 061000, China

  • Online:2022-03-25 Published:2022-04-14

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Abstract:

Objective To identify genes and miRNAs by microRNA(miRNA)-mRNA interaction network in idiopathic pulmonary fibrosis(IPF).

Methods The data of patients with IPF that were downloaded from the gene expression omnibus（GEO）database (GSE27430 and GSE135065) were used to select differentially expressed miRNAs and mRNAs. Transcription factor enrichment analysis was used to select miRNAs, and miRDB, miRTarBase and TargetScan were used to filter miRNA-targeting mRNAs. Cytoscape software was used to visualize the network between miRNA and mRNA and calculate the Hub gene. The GO and KEGG pathways were used to analyze mRNA in the regulatory network.

Results A total of 21 miRNAs and 119 mRNAs were selected, and we reconstructed a miRNA-mRNA network composed of 3 miRNAs and 3 mRNAs. hsa-miR-199a-5p, hsa-miR-299-5p, hsa-miR-338-3p, NTNG1, SCN2A, and TM6SF1 were identified as key regulators.

Conclusion This study has found several important Hub genes and miRNAs involved in the pathogenesis of IPF. Among them, the hsa-miR-199a-5p/NTNG1 and hsa-miR-299-5p/SCN2A pathways may be involved in the pathogenesis of IPF, which can help identify the cause and provide potential therapeutic targets.

Key words: idiopathic pulmonary fibrosis, gene, microRNAs