Abstract: ［Abstract］Objective〖HTSS〗To investigate effects of 17βestradiol(E2) on cell proliferation of ovarian clear cell carcinoma cell line ES2, and to explore its possible mechanism.
〖HTH〗〖WTHZ〗Methods〖HTSS〗The experiment was divided into E2 10-7mol/L group, E2 10-8mol/L group, E2 10-9mol/L group, E2 10-10mol/L group, E2 10-11mol/L group, solvent control group and blank control group. The proliferative activity of different E2 concentrations at different time(24, 48, 72 h) was measured by MTS method and expressed as absorbance(optical density OD) value, the invasive capability was detected after treated with different concentrations of E2 through transwell experiment. The expression of AT rich interaction domain 1A(ARID1A) protein，p53 and matrix metalloproteinase9(MMP9) protein in ES2 cells treated with different concentrations of E2 was measured by western blot.
〖HTH〗〖WTHZ〗Results〖HTSS〗The proliferative activity of E2 group cells after treated with different concentrations of E2 for 24, 48, 72 hours was higher than solvent control group and blank control group(P＜0.05). The transwell experiment showed that the number of transmembrane cells in the E2 group were significantly higer than that in the control group(P＜0.05). Western blot analysis showed that with the increase of E2 concentration, the expression of ARID1A decreased gradually compared with the control group, while the expression of MMP9 and p53 increased with the increased(P＜0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗E2 could downregulate the expressions of ARID1A protein, upregulate the expressions of p53 and MMP9 protein. E2 could enhance the ability of proliferation and invasion of ES2 cell. Patients with clear cell carcinoma of the ovary who had undergone surgery should be more cautious in the use of estrogen therapy.

Key words: ovarian neoplasms, estradiol, cell proliferation