Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the expression of CC class chemokines 22(CCL22) and the specific marker of regulatory T cells(Tregs) forkhead/winged helix transcription factor 3(FOXP3) and to analyze the correlation and the relationship with clinicopathological factors and prognosis in order to explore the effect of Tregs on tumor immunity in cholangiocarcinoma.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The expression of CCL22 and FOXP3 in 70 cases of cholangiocarcinoma and 15 cases of paracancer were detected by immunohistochemical staining.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The positive expression rates of CCL22 and FOXP3 in 70 cases of cholangiocarcinoma， 15 cases of nontumor bile duct epithelium were 62.4%, 20.0% and 71.4%, 13.3%, respectively. There was a significant difference among different groups(P＜001). The positive rates of CCL22 and FOXP3 were significantly higher in the stage Ⅲ+Ⅳ and lymph nodes metastasis patients than those in stage Ⅰ+Ⅱ and no lymph nodes metastasis patients（P＜005）. The expression of CCL22 was positively correlated with that of FOXP3(P＜0.01). Moreover survival time of negative expression in CCL22 and FOXP3 patients was longer than that of positive expression in CCL22 and FOXP3 patients（P＜001）.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓CCL22 and FOXP3 may have great influence on  development and prognosis of cholangiocarcinoma.

Key words: bile duct neoplasms, CC class chemokines 22, forkhead/winged helix transcription factor 3