Abstract: ［Abstract］〓Objective〖HTSS〗〓To study the expression of growth arrest specific6(Gas6) and matrix metallo proteinase9（MMP9） in cholangiocarcinoma and its correlation with clinical parameters.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The expression of Gas6 and MMP9 protein was detected by immunohistochemistry(SP) in 60 cases of cholangiocarcinoma and 17 cases of paracancerous bile ducts, combined with the clinical parameters of patients(age, tumor differentiation, tumor size, with or without lymph node metastasis, with or without hepatic artery and portal vein invasion) and other clinical factors for comprehensive analysis.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The positive expression rate of Gas6 in cholangiocarcinoma was 71.7%, which was higher than that in paracancerous tissues(29.4%)(P＜005). The positive expression rate of Gas6 in cholangiocarcinoma was correlated with lymph node metastasis and hepatic artery or portal vein invasion(P＜005). The positive expression rate of MMP9 in cholangiocarcinoma was 66.7%, which was higher than that in paracancerous tissues(35.3%)(P＜005). The expression of MMP9 in cholangiocarcinoma was correlated with lymph node metastasis and hepatic artery or portal vein invasion(P＜005). There was positive relationship between the expression of Gas6 and MMP9(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The expression of Gas6 and MMP9 was closly associated with the development and progression of cholangiocarcinoma.

Key words: bile duct neoplasms, growth arrest specific6, matrix metallo proteinase9