Abstract: ［Abstract］ Objective〖HTSS〗〓To explore the effects of Toll like receptor 4(TLR4)matrix metalloprotein2(MMP2)/matrix metalloprotein9(MMP9) signal pathway on apoptosis of human umbilical vein endothelial(HUVEC) induced by oxygenglucose deprivation/resuscitation.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓In vitro, HUVEC12 cells which was exposed to oxygen and glucose(OGD) for 6 h and 〖JP2〗reperfusion for 24 h were divided into 4 groups: control(C) group, OGD/R(O) group, TAK242+〖JP〗OGD/R(TO) group, CCT+OGD/R(MO) group. Before OGD exposure, the cultures in TO and MO groups were treated with TLR4 inhibitor TAK242(10 μmol/mL) or MMP2/MMP9 inhibitor CCT(200 μg/mL), respectively. The effects of apoptosis and viability were assessed by Annexin VFITC/PI and MTT assays. The resistance and permeability were assessed by Transwell assay. The levels of TNFα and IL1β were assessed by ELISA. The expressions of MMP2 and MMP9 were assessed by western blot measurement.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with group C, the apoptosis rate and glucanFITC fluorescence intensity of HUVEC12 cells in group O, TO and MO increased, while the survival rate and resistance decreased, and the expression levels of TNFa, IL1β, MMP2 and MMP9 increased(P＜005). Compared with O group, the apoptosis rate and fluorescence intensity of glucanFITC in TO group decreased, the survival rate and resistance increased, and the expression levels of TNFα, IL1β, MMP2 and MMP9 decreased(P＜005). Compared with group O, the apoptosis rate of HUVEC12 cells and the fluorescence intensity of dextranFITC in MO group were decreased, the survival rate and resistance value were increased, and the expression of TNFα and IL1β in water skin was decreased(P＜005). Compared with TO group, the apoptosis rate of HUVEC12 cells and the fluorescence intensity of dextranFITC in MO group increased, the survival rate and resistance value decreased, and the expression of TNFα, IL1β, MMP2 and MMP9 in the water skin increased(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Apoptosis of HUVEC12 cells induced by oxygenglucose deprivationreoxygenation may be related to activation of TLR4MMP2/MMP9 signaling pathway.

Key words: anoxia； human umbilical vein endothelial cells, apoptosis