Abstract: ［Abstract］〓Objective〖HTSS〗〓To investigate the feasibility of GATA4 gene promote 5azacytidine（5aza） induced differentiation of bone marrow mesenchymal stem cells(BMSCs) into cardiomyocytes.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Rat BMSCs were isolated from the whole bone marrow and cultured.Using the cationic liposome reagent, Lipofectamine 2000,the plasmid pVP22GATA4/mycHis was transfected into P3 BMSCs at a ratio of 1〖DK〗∶2.5. After 72 h,BMSCs was induced by 10 μmol/L 5aza for 24 h.The whole experiments were observed under an inverted fluorescence microscope and expression of GATA4 was examined with immunocytochemistry. Expression of cardiac troponin T(cTnT) and GATA4 were determined with Western blotting and immunocytochemistry.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓After 48 h of transfection, GATA4、myc were expressed in experimental group. After 28 days, immunocytochemistry and Western blotting results indicated that the expression of GATA4 and cTnT in experimental group was significantly higher than those of control group（P＜005）.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓GATA4 gene may promote 5aza induced differentiation of  BMSCs into cardiomyocytes.

Key words: mesenchymal stem cells, bone marrow, GATA4 transcription factor, cell differentiation