Abstract: ［Abstract］〓Objective〖HTSS〗〓To investigate the role of Bone Marrow Mesenchymal Stem Cells(BMSCs) that overexpression GATA4 gene in differentiating into cardiomyocytes.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Rat BMSCs were isolated from the whole bone marrow and cultured.After multiple passages and purification，the GATA4 (pVP22GATA4/mycHis) gene was transiently transfected into SD rat BMSCs by liposome transfection with the help of the cellpenetrating peptide VP22. After 3 days, BMSCs was induced by a suitable concentration of 5aza for 1 day. The expression of GATA4 and myc were examined by Western blotting. Expression of cardiac troponin T(cTnT) and GATA4 were determined with immunocytochemistry and Western blotting after 21 days.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓GATA4 and myc were expressed in experimental group after 2 days of transfection. After 21 days, Western blotting and immunocytochemistry results indicated that the expression of GATA4 and cTnT in experimental group was significantly higher than those other groups.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Overexpression GATA4 gene could promote BMSCs differentiate into cardiomyocytes during 5aza induction.

Key words: mesenchymal stem cells, bone marrow, myocytes, cardiac, GATA4