Abstract: Objective In order to provide experimental evidence for the comprehensive application of compound T44,we explored the possible mechanisms of its anti-tumor effects. Methods MTT assay was used to measure cell proliferation. Transition of cell cycle and apoptosis were tested by flow cytometer,respectively. Capacity of cell migration was detected by scratch assay. Western blotting was applied for determining the expression of different cyclins. Detection of cell morphology change was utilized to observe the influence on HCT116 cells. Results The proliferation of 11 kinds of tumor cell lines were decreased markedly as compared with those of control cells,and they showed distinct sensibility with one another. Half maximal(50% )inhibitory concemtration of human lung fibroblasts was(16. 58 ± 3. 49)μmol/ L,higher than most of the tumor cell lines. T44 treated HCT116 cells decreased cell migration and altered cyclins expression,as well. T44 could also lead to cell cycle block and apoptosis. Conclusion T44 participated into the control of tumor proliferation probably by modulation of cell cycle and induction of apoptosis.

Key words: antineoplastic agents, cell proliferation, cell cycle