Abstract: ［Abstract］ Objective〖HTSS〗To compare two detection methods of goat-milk containing anti-Helicobacter pylori specific antibody, and to observe the clinical effect of antibody goat-milk.
Methods〖HTSS〗Goat-milk containing antibodies against Helicobacter pylori was prepared by immunizing dairy goat with international standard strains (ATCC26695, ATCC26695 and SS1 mixture). The valence of antibody against Helicobacter pylori in goat-milk was detected by double immunodiffusion test and enzyme linked immunosorbent assay(ELISA), and then compare the two methods. 30 subjects were randomly assigned to two groups. The test group were treated with goat-milk containing Helicobacter pylori specific antibody, and control group with common goat-milk, drink for 30 days continuously. The effect was assessed by the 14C urea breath test(14C-UBT).
Results〖HTSS〗The results of the two methods all confirmed the existence of anti-Helicobacter pylori specific antibodies in goat milk, ELISA test show that the content of antibodies in the two goat-milk was higher than that in the control, the difference was statistically significant(P＜0.05), and with the increase of dilution ratio, the antibody content decreased gradually. There was no significant difference in antibody content between the two kinds of goat milk(P＞0.05). Preliminary clinical trials have shown that the effective rate of the experimental group was 66.6% after taking the antibody goat-milk, and the difference was statistically significant(P＜0.05) compared with the control group.
Conclusion〖HTSS〗Both of the two detection methods can better prove the existence of anti-Helicobacter pylori specific antibodies in goat-milk. Methed of ELISA is simple and fast, which can more accurate quantitatively detect antibody content. And the results are more reliable. Clinical trials have shown that goat-milk containing Helicobacter pylori specific antibody can effectively reduce the value of 14C-UBT in patients, it has a good prospect of clinical application.

Key words: Helicobacter pylori, anti-Hp specific antibody, goat-milk containing antibody