Abstract: Objective A high efficiency liquid chromatography method was established for the determination of principal component crude drug josamycin.Methods A octadecyl bonded silica column was used as the stationary phase,with the mobile phase of mixture of perchloric acid solution-acetonitrile (60∶40)at the detection wavelength of 231nm.The column temperature was 40℃ and the injection volume was 20μL.Results The principal component of josamycin showed a linear range within 0.125 5- 0.878 5g/L (r = 1.000 0).The average recovery was 99.7%.The quantitation and detection limits were 2.51ng and 0.84ng,respectively.Conclusion The method was rapid,simple,accurate,sensitive,and suitable for the determination of principal component in crude drug josamycin.

Key words: josamycin, chromatography, high pressure liquid, quality control