Abstract: ［Abstract］  Objective〖HTSS〗〓To explore the mechanism and the significance of different methods to establish scoliosis model of bipedal rats.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Forty SD rats were randomly divided into four groups. The front limb and tail of SD rats was completely removed. The control group only create model of bipedal rats. Ten bipedal rats in group A were given intraperitoneal injection of 6mercaptopurine(30 mL/kg). Tenbipedal rats in group B received intraperitoneal injection of luzindole(0.2 mg/kg). 10 bipedal rats in group C received intramuscular injection of botulinum toxin A. The spinal curvature index, change of intervertebral height and the characteristics of scoliosis were compared between the four groups before and after operation.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓All the experimental animals survived to complete the modeling experiment of scoliosis. In control group, there was no scoliosis. All three experimental groups had scoliosis. Cobb's angle of rats were all increased in four groups and showed an raising trend. However, cobb's angle of the 6 mercaptopurine group increased the maximum. Vertebral height of rats in four groups were all increased and showed a rising trend. Compared with control group, the height of vertebral body was lost in 6mercaptopurine group and botulinum toxin group. However, the vertebral height of low melatonin group height was increased. There were significant differences among groups, time points, interaction of group and pointintime(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Application of intraperitoneal injection 6mercaptopurine can establish a severe scoliosis model. Intraperitoneal injection of luzindole proved that low concentration of melatonin can induce scoliosis. The botulin can damage the fiber tissue of paraspinal muscle. It is proved that destruction of biomechanical balance of paraspinal muscle can creat scoliosis models.

Key words: scoliosis, models, animal, rats