Journal of Hebei Medical University ›› 2021, Vol. 42 ›› Issue (4): 454-460,465.doi: 10.3969/j.issn.1007-3205.2021.04.018

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Effects of intravenous anesthesia with sufentanil combined with propofolin bone marrow mesenchymal stem cell transplantation on rats with traumatic brain injury

  

  1. 1.Department of Anesthesiology, People′s Hospital of Longhua District, Guangdong Province, 
    Shenzhen 518109, China; 2.Department of Anesthesiology, the Sixth Affiliated Hospital of 
    Xinjiang Medical University, Xinjiang, Urumqi 830002, China
  • Online:2021-04-25 Published:2021-04-29

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Abstract: Objective  To investigate the effect of intravenous anesthesia with propofol and sufentanil in bone marrow mesenchymal stem cell transplantation on the treatment of traumatic brain injury(TBI) in rats. 
Methods  Bone marrow mesenchymal stem cells were isolated from young rats aged 3 weeks and cultured in vitro to 3 generations. A total of 45 healthy female SD rats were randomly divided into control group, stem cell treatment group and combination treatment group, with 15 rats in each group. TBI models were established by electric cortical contusion impactor(eCCI). After establishment of TBI modeling, the rats in stem cell treatment group were given 0.5 mL stem cell suspension(1×105/mL), the rats in combination treatment group were given stem cell suspension plus injection of 0.5 g/kg sufentanil and 2.0 mg/kg propofol, while the control group rats were given an equal volume of saline. Modified neurological function scores(mNSS) was performed to determine the degree of nerve damage in rats during treatment; morphological changes of brain tissue were observed by HE staining. The water content of brain, total calcium content and serum level of S100β were measured by dry-and-wet gravity method, spectral scanner and enzyme linked immunosorbene assay. Neuronal apoptosis in hippocampal structural region was observed by TUNEL staining, western blot was used to detected the expression of apoptosis-related proteins, and quantitative real-time(qRT-PCR) and immunohistochemistry were used to detect the expression and distribution of inflammatory factors in brain tissues. 


Results  After passage, the cells showed the trend of radial or vortex-like colony growth, and the cell morphology gradually became stable and uniform with the increase of passage times. Finally, the cells grew stably into spindle-like fibroblasts. Within 3 days after TBI modeling, there was no significant difference in mNSS score among the three groups(P＞0.05); At 7 days after treatment, the mNSS score of the combination treatment group was significantly lower than that of the control group; At 14 and 21 days after treatment, the scores of the stem cell treatment group and the combination treatment group were significantly lower than that of the control group, and the scores were lower in combination treatment group than in the stem cell treatment group(P＜0.05). Compared with the control group, the pathological changes of the hippocampus in the stem cell treatment group and the combination treatment group were significantly improved, and the density of nerve cells in the brain tissue of the combination treatment group was significantly restored. In addition, the cells in the hippocampus were orderly arranged, the nucleus was complete and the staining was lighter, which was better than the stem cell treatment group. The brain water content, total calcium content and serum S100β content in stem cell treatment group and combination treatment group were lower than those in control group, and the indicators were lower in combination treatment group than in stem cell treatment group(P＜0.05). The number of apoptotic cells and the expression of Caspase-3, Bax and cytc protein in brain tissue of stem cell treatment group and combination treatment group were lower than those of control group, while the indicators were lower in combination treatment group than in the stem cell treatment group. The expression of Bcl-2 protein in stem cell treatment group and combination treatment group was higher than that of control group, and the expression was higher in the combination treatment group than in the stem cell treatment group(P＜0.05). The mRNA expression levels of IL-6, IL-1 β and TNF-α in the stem cell treatment group and the combination treatment group were lower than those in the control group, which were lower in the combination treatment group than in the stem cell treatment group(P＜0.05). 
Conclusion  Intravenous anesthesia with sufentanil and propofol in bone marrow mesenchymal stem cell transplantation can promote the recovery of neurological function after TBI, by reducing brain water content, total calcium content and serum S100β accumulation in rats, and inhibiting the neuronal apoptosis and expression of inflammatory factors.

Key words: brain injuries, sufentanil, propofol, bone marrow mesenchymal stem cell