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    25 January 2016, Volume 37 Issue 1
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    Establishmentofculturesystemofmousespermatogeniccellsinvitrowithoutserum
    GONG Miao,ZHANGLei 1,ZHAOYu 1,YINQing 1,GUOQian-yu 1,RENGuang-wei 2*
    2016, 37(1):  1-4.  doi:10.3969/j.issn.1007-3205.2016.01.001
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     Objective ToestablishamouseLeydigcell-Sertolicell-germcelldouble-chamber
    coculturesysteminvitro withoutserum.Methods TheLeydigcellswereisolatedfromthe
    testesofpostnatalday60maleC57BL / 6mice.TotalgermcellsandSertolicellswereisolated
    fromthetestesofpostnatalday15maleC57BL / 6mice.Thecellsinbicameralchambersculture
    system weregrowninmediawithoutserum.Themorphologyandgrowthofculturecellswere
    monitoreddailyundercontrastphasemicroscope , andwereidentifiedbyHematoxylinstaining.
    Ploidyanalysisofcellswasobservedbyflowcytometry.Results Inbicameralculturesystem ,
    sporadicround spermatids were detected after one week , and elongating spermatids or
    spermatidswithflagellawereobservedafterthreeweeks.Thespermatogeniccellssurvivedas
    longaseightweeks.Thehaploidpeakwasdetectedafteroneweek ; thepercentageofmonoploid
    increasedwithculturetime.Conclusion Thespermatogeniccellsinbicameralculturesystem
    maturedintomorphologicallynormalspermatozoaandsurvivedlonger.
    CardiaceffectsoftheC.1583C>G LMNA mutationintwofamilies#br# withEmery-Dreifussmusculardystrophy
    ZHANGLi,ZHANGLi-xuan,ZHANGSu-hua,WANGJing,DINGFang
    2016, 37(1):  5-8,12.  doi:10.3969/j.issn.1007-3205.2016.01.002
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     Objective ToreportandanalyzecardiacinvolvementintwoEmery-Dreifuss
    musculardystrophy ( EDMD ) pedigreescausedbyC.1583C>G mutationofthelaminA
    / Cgene
    (
    LMNA ) .Methods ThecharacteristicsofmembersoftwofamilieswithEDMDwereevaluated
    clinically , pathologicallyandgenetically.Skeletalmusclebiopsiesandpathologicalanalysiswere
    performedintwoprobands.Electrocardiogram , ultrasoundcardiographyand 99 Tc
    M
    -MIBI-gated
    myocardiacperfusionimaging (
    99 Tc M
    -MIBIGPI ) wereperformedonthreepatients.Results
    Familyhistoryinvestigationsrevealedanautosomaldominanttransmissionpatternofdiseasein
    family1 , andasporadiccaseinfamily2.Threeaffectedpatientsallpresentedtypicalclinical
    featuresof EDMDincludingjointcontracture , muscle weakness , andcardiacinvolvement.
    Musclehistopathologicalstudyrevealeddystrophicfeatures.Moreover , eachaffectedindividual
    presentedwithcardiacarrhythmia , evidentassinustachycardia , atrialflutter , orcomplete
    atrioventricularblock.Cardiacimagingstudyshoweddilatedcardiomyopathyintwopatients 
    oneofwhom wasundergoingheartfailure , thesecondpatienthadnoobviousabnormalitiesin
    cardiacstructureorfunction.Allthreeaffectedindividualshadaheterozygousmissensemutation
    in LMNA gene ( C.1583C>G ), whichcausedaT528Raminoacidchangein LMNA protein.
    Conclusion Three patients were identified with EDMD , clinically , pathologically and
    genetically.CausativegenewasmissensemutationC.1583C>Gof LMNA .EDMDcausedby
    mutationof LMNA presented moreseverecardiacinvolvement , complicated with cardiac
    conductionsystemdefect , cardiomyopathyand / orheartfailure.
    Clinical effectiveness of endovascular therapy for total occlusion of the subclavian arteries
    LIU Yajun1,3,WANG Di2,ZHAO Weidong1,GAN Quan1,ZHAO Guozhen1，ZHANG Jian3*
    2016, 37(1):  9-12.  doi:10.3969/j.issn.10073205.2016.01.003
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    ] Objective Thepurposeofthisstudywastoevaluatetheeffectivenessofstenting
    therapyforthepatientswithtotalsubclavianarteryocclusion.Methods Atotalof67patients
    hadunderwentendovasculartherapyforthetreatmentoftotalsubclavianarteryocclusion.All
    patientswerefollowedat3 , 6 , and12monthsaftertreatmentandannuallythereafter.Results
    52stentswereplaced.Technicalfailurewasnotedin15patients , whilethesymptomsfailedto
    resolveintwo.Therewerenoperioperativedeaths.4complicationswereobserved.Therateof
    primarypatencywas98.22% at12 months , 94.31% at24 months , and92.17% at3years.
    Significantrecurrentobstructiondevelopedinfivepatientswhousedballoon-expandablestents.
    Conclusion Whenperformedbyskilledsurgeons , endovasculartherapywithstentplacement
    canbeusedasasafeandeffectivetherapyfortotalocclusionoftheproximalsubclavianartery.
    However , embolicprotectiondevicesshouldbeconsideredduringthemanagementofrightside
    occlusion.
    Efficacyandsafetyofspironolactoneinsystolicheartfailurepatientswithmildrenaldysfunction
    GENGTao,DAIShi-peng,ZHANGJian-gang,XUZe-sheng
    2016, 37(1):  13-17.  doi:10.3969/j.issn.10073205.2016.01.004
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     Objective Toinvestigatetheefficacyandsafetyofspironolactoneinsystolic
    heartfailurepatientswithmildrenaldysfunction.Methods Atotalof118systolicheartfailure
    patientswith mildrenaldysfunction ( glomerularfiltrationrate [ GFR ] 60-90 mL · min
    -1 ·
    1. 73m -2 ) wererandomlydividedintotwogroups : controlgroupincluded58cases , given
    conventionaltreatment ; spironolactonegroupincluded60cases , treatedwithspironolactone20
    mg
    / donthebasisofconventionaltherapy.Allpatientswereassessedwithechocardiographyfor
    leftventricularejectionfraction ( LVEF ), leftventricularmassindex ( LVMI ), leftventricularend
    diastolicvolumeindex ( LVMI ) andleftventricularendsystolicvolumeindex ( LVEDVI ) before
    treatmentand12 monthsaftertreatment.Results Beforetreatment , twogroupsshowedno
    significantdifferenceinLVEF , LVMI , LVEDVIandLVESVI ( P >0. 05 ) .In12 monthsafter
    treatment , LVEF , LVMI , LVEDVIandLVESVIwereamelioratedobviouslyintwogroups
    ( P <0.
    05 ) .Compared with control group , spironolactone group was ameliorated more
    significantly ( P <0. 05 ) .Therewerenosignificantincreaseinhyperkalemia
    ( Kalemia>5.
    5mmol
    / L ) in spironolactonegroup ( P >0. 05 ) .Therewerenoseverehyperkalemia (
    Kalemia≥6.0mmol
    / L ) in
    twogroups.Thereweresignificantincreaseinhypokalemia ( Kalemia<3.5mmol
    / L ) incontrol
    groupcompared with spironolactone group ( P <0. 05 ) .There weresignificantincreasein
    gynecomastiaormastodyniainspironolactonegroupcomparedwithcontrolgroup ( P <0. 05 ) .
    25%increaseinGFRhad2patientsineachgroup , therewerenostatisticalsignificanceintwo
    groups ( P <0. 05 ) .Therewerenopatientsintwogroupsrequiringdialysistreatmentbecauseof
    worseningrenalfunction.Conclusion Spironolactoneinadditiontotheroutinetreatmentfor
    systolicheartfailurepatients with mildrenaldysfunctioncanfurtherameliorateventricular
    remodeling , decreasetheincidenceofhypokalemia.Itwassafeinrigorousmonitor.
    Standardizedtreatmentanalysisofacutecarbonmonoxidepoisoning
    WULi-ping,SUYue-nan,ZHANGYi-wei
    2016, 37(1):  18-20.  doi:10.3969/j.issn.10073205.2016.01.005
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    Objective Toimprovehandlingofacutecarbon monoxidepoisoning ( ACOP ) .
    Methods Atotalof183casesofACOPpatientsweredividedintocontrolgroup85casesand
    observationgroup98casesbytreatmenttime.Theobservationgroupwastreatedbystandardized
    treatment , whilethecontrolgroup was by conventionaltreatment.Theclinicalefficacy ,
    consciousnessrecoverytimeandtheincidenceofdelayedencephalopathyoftwogroupswere
    compared.Results Clinicalefficacyoftwogroupswasnotstatisticallysignificant ( P >0. 05 ),
    whileaverageconsciousnessrecoverytimeofmoderateandseverepatientsinobservationgroup
    wasshorterthanthatofcontrolgroup ( P <0. 05 ), andtheincidenceofdelayedencephalopathyof
    observationgroupwaslowerthanthatofcontrolgroup ( P <0. 05 ) .Conclusion Applicationof
    standardizedtreatmentofACOPcontributestorapidtreatmentandreducestheincidenceof
    delayedencephalopathyofACOP.
    Diagnosis and treatment of incarcerated gallstones in emergency department which caused 180 cases of acute abdominal pain
    HUANG Chenhai,WU Zhiyi,MO Weilin
    2016, 37(1):  21-23,34.  doi:10.3969/j.issn.10073205.2016.01.006
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    ] Objective Tosummarizeandanalyzetheassociatedinducingfactorsofacute
    abdominalpaincausedbyincarceratedgallstonesandcholecystectomy ( LC ) embeddedapplication
    valueinthetreatmentofgallbladderstones.Methods Thestudyenrolled180patientswith
    acuteabdominalpaincausedbyincarceratedgallstoneshospitalizedfromemergencydepartment
    ofourhospitalastheresearchobjects , accordingtothesurgicalprocedure , thepatientswere
    dividedintotwogroups : 130casesinresectiongroup , and50casesingallbladderpreserving
    group.Resection group was given LC treatment , gallbladder preserving group was given
    gallbladderpreservingandstoneextractionfortreatment.Therelatedfactorsandthelengthof
    hospitalstay , postoperativerecoverytime , calculirecurrencerate
    , incidenceofpostoperative
    complicationsofpatientsintwogroupswerecompared.Results Singlefactoranalysisshowed
    thatthehistoryofabnormalliverfunction , gallbladderenlargement , maximumgallstone>1.25
    cm and diabetes history wererisksforincarcerated gallstones.There were no significant
    differencesinthelengthofhospitalstay , timeofpostoperativerecoverytimeandtheincidenceof
    complicationsbetween resection group and gallbladder preserving group ( P >0.05 ) .The
    recurrencerateofresectiongroupwaslowerthanthatofgallbladderpreservinggroup ( P <0.01 )
    Effect of raltitrexed combined oxaliplantin as secondline chemotherapy for advanced gastric cardial carcinoma
    LV Huilai1，ZHANG Li2，WEN Shiwang1，LI Yong1，ZHANG Yuefeng1，TIAN Ziqiang1*
    2016, 37(1):  24-26.  doi:10.3969/j.issn.10073205.2016.01.007
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     Objective Toevaluatetheefficiency andtoxicity ofraltitrexedcombined
    qxaliplantinassecond-linechemotherapyintreatmentofadvancedgastriccardialcarcinoma.
    Methods Fifty-eightpatientsofadvancedgastriccardialcarcinomawhohadreceivedfirst-line
    therapybuthadrelapsedorprogressedweregiven3mg
    / m
    2 ofraltitrexed ,
    intravenousbolus ,
    d1 , 21daysasacycle , combinedoxaliplantin130mg / m
    2 ,
    intravenousinfusion>3h , d1 , 21
    daysasacycle.Theanalysislastedatleasttwocyclessoastoevaluatetheshort-termefficiency
    andadversereactions.Aftertwocyclesofchemotherapy , theefficiencyandtoxicity were
    evaluated.Atotalofsixcycleswereappliedunlesstherewereevidenceofdiseaseprogressionor
    intoleranceoftreatment.Results All58patientswereevaluable , includingcompleteresponse2
    cases , accountingfor3.4% , partialresponse16cases , accountingfor27.6% ; stabledisease14
    cases , accountingfor24.1% ; progressivedisease26cases , 44.8%.Overallresponseratewas
    31.0% , diseasecontrolrate55.2% , themiddletimetoprogression6.9months.Themainside
    effects were myelosuppression , gastrointestinal reactions , aminotransferase abnormality ,
    peripheralneurotoxicityandsoon.Thewholegrouphadnotreatment-relateddeathinpatients
    and no toxicity due to the delay chemotherapy.Conclusion Raltitrexed as second-line chemotherapyintreatmentofadvancedgastriccardialcarcinomaiseffectiveandwelltolerated.
    Earlyrehabilitationnursingofneonatalobstetricbrachialplexusinjury
    LIUSu-zhe,ZHAOShuo,ZHAO Wen-ying,WANGJing-ming,LIU Qian,MENGZhao
    2016, 37(1):  27-29.  doi:10.3969/j.issn.10073205.2016.01.008
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    ] Objective Toexploreearlyrehabilitationnursinginneonatalobstetricbrachial
    plexusinjury.Methods Fifty-six children with neonatalobstetric brachialplexusinjury
    experiencedearlystageexplorationlysis , andlater , theywererandomlydividedintocontrol
    groupandrehabilitationgroup , 28 patientsinagroup.Thechildrenincontrolgroup got
    conventionaltreatment , includingneurotrophicdrugs , selffunctionaltrainingathome ; The
    childreninrehabilitationgroupwereimplementedwithlow-frequencyneuromuscularelectrical
    stimulationandnormalizationofmotorfunctiontraining , comprehensivecaremeasuresonthe
    basisofconventionaltreatment.Accordingtothebrachialplexusfunctionevaluationcriteria ,
    thetwogroupsofchildren wereevaluatedinlimbfunctionrecovery.Results Rehabilitation
    grouphadfunctionalrecoveryexcellencein13cases , goodin9cases , effect4cases , andpoorin
    2cases ; thecontrolgroupshowedexcellencein5cases , goodin8cases , effectin8cases , and
    poorin7cases.Theefficacyofrehabilitationgroupwasbetterthanthatofcontrolgroup , the
    prognosisinexcellenceandgoodgradeweresignificantlyhigherthanthatofcontrolgroup ( P <
    0. 05 ) .Conclusion Implementationoflow-frequencyelectricalstimulationofneuromuscular
    trainingcombinedwithstandardized motorfunctionintegratedcaremeasurescansignificantly
    improveneonatalobstetricbrachialplexusinjuryandraisetreatmentgoodrate , enhancethe
    functionalactivity and prognosisin children , reducefamily anxiety , which worth clinical
    application.
    Correlation of mindfulness level with selfperceived burden and family rehabilitation in elderly patients with hip fracture fixation
    FU Changzhong1,HAO Zhengwei2,GE Yanlei2
    2016, 37(1):  30-34.  doi:10.3969/j.issn.10073205.2016.01.009
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     Objective Toinvestigatethe mindfulnesslevelanditscorrelation withself-
    perceivedburdenandfamilyrehabilitationinelderlypatientswithhipfracturefixation.Methods
    Mindful Attention Awareness Scale , Compliance Scale and Self-perceived Burden Scale
    (
    SPBS ) wereusedasevaluatingtoolstoanalyzethemindfulnesslevel , complianceandself-
    perceivedburdenfortotally168casesofelderlypatientswithhipfracturefixation ; andits
    correlation withself-perceived burdenandfamilyrehabilitationinelderlypatients with hip
    fracturefixationcombiningthepatients'hipfunction ( HarrisScale ) and Daily Life Ability
    (
    Barthelindex ) wereanalyzedbyPearson.Results Thefamilyrehabilitationconditioninthe
    highmindfulnesslevelgroup wasobviouslyhigherthaninlow mindfulnesslevelgroup ( P <
    0. 05 ) .Logisticregressionanalysisshowedthatgender , age , chronicdiseasesaffectingbone
    metabolism , operationtime , theleveloffallrisk , SPBSandthefamilyfunction werethe
    influencingfactorsforpatientswithhipfracturefixation.Thecompliancebehaviorssuchassmoking , drinkinganddiet
    , medication , rehabilitationexerciseandpostureprotectioninhigh
    mindfulnesslevelgroupwereobviouslybetterthaninlow mindfulnesslevelgroup ( P <0. 05 ) .
    Pearsonanalysisshowedthattotalmindfulnessscoreandeachsingleitemscorewerenegative
    correlatedwithSPBS ( all P <0. 01 ) .Conclusion Themindfulnesslevelofelderlyhipfracture
    fixationis higher , the self perceived burdenislighter and compliance higher , whichis
    advantageoustofamilyrehabilitation.
    Clinical diagnostic value of cells free ferrous protoporphyrin and thincytologic test for cervical lesions
    HU Yuqin,LIU Fuqiang*,LIU Yanli,LIU Ping,LIANG Weiguo,LI Xiaoli
    2016, 37(1):  35-39.  doi:10.3969/j.issn.10073205.2016.01.010
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     Objective Toinvestigatetheclinicalvalueofcellsfreeferrousprotoporphyrin
    (
    FH ) andthin-cytologictest ( TCT ) inthescreeningofcervicallesions.Methods FH wasused
    toexamine100patientswhohadbothTCTresultsandpathologicalexaminationresults.Based
    ongoldstandardofpathologicalexamination ( >CIN Ⅰ wasdefinedaspositive ), thesensitivity ,
    specificity , accuracy , positivepredictivevalue
    , negativepredictivevalueofFHtestandTCT
    were calculated , respectively.Results The sensitivity , specificity , accuracy , positive
    predictivevalue , negativepredictivevalueofFH were82.
    9% ( 58 / 70 ), 70. 0% ( 21 / 30 ), 79. 0%
    (
    79 / 100 ), 86. 5% ( 58 / 67 ) and63. 6% ( 21 / 33 ) .Thesensitivity , specificity , accuracy , positive
    predictivevalue , negativepredictivevalueofTCTwere87.
    1% ( 61 / 70 ), 76. 6% ( 23 / 30 ), 84. 0%
    (
    84 / 100 ), 89. 7% ( 61 / 68 ) and71. 9% ( 23 / 32 ) .Theeffectivenessoftwomethodshadnostatistical
    significance ( P >0. 05 ) .Theefficiencyincreasedwhenthetwotestmethodsjointed.Conclusion
    FHtestasanew methodforcervicallesionsscreeningissimple , economic , andhassame
    clinicalvalueasTCT.ThemethodFHjointingTCTforcervicallesionsscreeninghashigher efficiency.
    Bacteria detection in sputum and analysis of bacterial resistance in childhood pneumonia
    HUANG Guangli,SHI Qingsheng,CHEN Haixia,CHE Xiangyu,LI Junqin
    2016, 37(1):  40-43.  doi:10.3969/j.issn.1007-3205.2016.01.011
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    ] Objective Toinvestigatebacterialinfectionanddrugresistanceinchildrenwith
    community-acquiredpneumonia ( CAP ), andtoprovidethebasisforearlyclinicaldrugtherapy.
    Methods Theworkinvestigated1328CAPaffectedinfantsinourhospital.Theirsputum
    samplesweregotthroughasepticnegativepressureattractionandthenbacterialcultivationwas
    madewithagardilution.ThebacterialdrugresistancewasmeasuredthroughbothKirby-Bauer
    discdiffusionandbrothdilutionmethods.Results Theresultsrevealed676positiveinfantsand
    thepositiverate50.9%.Thetopfivebacteriaranged : Streptococcuspneumonia ( 204 / 680 ),
    Klebsiellapneumonia ( 130 / 680 , including8 ESBLs ), Escherichiacoli ( 104 / 680 , including5
    ESBLs ), Staphylococcusaureus ( 88 / 680 , including10MRAS ), andHaemophilusinfluenzae ( 36 /
    680 ) .TheanalysisofantibioticsusceptibilitytestrevealedthatStreptococcuspneumoniaingram-
    positivebacteriashowed19.6%resistanceratetothepenicillin ; Thedrugresistanceratewere
    12.7% toamoxicillin / clavulanaicacid.Meanwhile , Staphylococcusaureusshowed morethan
    90.0% drugresistanceratetopenicillinandamoxicillin / clavulanaicacid.Ontheotherhand ,
    cefotaximeandcotrimoxazole.But , theyshowedhighsensitivitytomeropenem.Conclusion
    Theobservationsrevealedthat CAPin ourhospital was mainlyinduced by Streptococcus
    pneumonia , Klebsiella
    pnueumoniae , Escherichia coli , Staphylococcus
    aureus. Most
    streptococcuspneumoniaaresensitivetopenicillinandamoxicillinclavulanatepotassium.The
    resultsareimportantfortestingpathogenicandusingantibioticreasonably , althoughthesputum
    samplesarelimitedtothejudgmentofpneumoniaetiology.
    gram-negativebacteriashowedhighdrugresistanceratetoampicillin , cephazolin , ceftriaxone ,
    Clinical test of serum treponema pallidum and human immunodeficiency virus in patients with oral lichen planus
    LIUTie-jun,SHITing-ting,LIUJian,ZHANGYu-wen,XUYan-zhi*
    2016, 37(1):  44-45,53.  doi:10.3969/j.issn.10073205.2016.01.012
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    ] Objective Toexploretreponemapallidum ( TP ) andhumanimmunodeficiency
    virus ( HIV ) infectionontheincidenceoforallichen planus ( OLP ) .Methods Automatic
    chemiluminescenceanalysis method wasusedto detectserum TP-IgG antibody , HIV-IgG
    antibodyexpressionbetweenOLPgroup ( n =100 ) andnormalcontrolgroup ( n =60 ) .Results
    SerumTP-IgGantibody , HIV-IgGantibodyexpressionshowednostatisticaldifferencebetween
    twogroups.Conclusion TPandHIVinfectionhadnoobviouseffectonpathogenesisofOLP.
    Valueofelectrocardiogramlimbleadinpredictingcoronaryarterycyclotronbranchlesion
    YU Qian1，ZHANG Kun1，SHI Wei1，YUAN Chen2，ZHANG Jun2*
    2016, 37(1):  46-49.  doi:10.3969/j.issn.10073205.2016.01.013
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    ] Objective Toevaluatetheclinicalvalueofelectrocardiogram ( ECG ) limbleadin
    predicting coronary artery cyclotron branch lesion.Methods Coronary angiography was
    conductedforatotalof615patientsdiagnosedascoronaryheartdisease , unstableanginapectoris
    including300cyclotronbranchlesionpatients , recordingtheirelectrocardiogramoftheresting
    andanginaattack , andpredictingthecyclotronbranchlesionthroughanalysisofresultsof
    coronaryangiography andthecharacteristic of ECG limblead.Results Thesensitivity ,
    specificityandaccuracyoflimbleadECGoftherestingwere36.00% , 84.76% and60.98%
    respectivelyforcyclotronbranchlesion.STsegmentdepressioninleadⅠ wasthemostprevalent
    inthechangeoflimbleadECG , accountingforabout90.22% ofECGepisodes ; STsegment
    depressioninlead Ⅱ andlead aVF were about67.39% and 67.39% of ECG episodes
    respectively ; ST segmentdepressioninlead Ⅲ occurredrarely
    , mostlynormalorslightly
    elevated , accountingforabout90.22% ofECGepisodes ; leadaVRhadnoabnormalchange
    mostly , accountingforabout70.65%inanginaattack.Conclusion Thespecificityoflimblead
    ECGoftherestingishigh , butthesensitivityislowforcyclotronbranchlesion.Itisvaluable
    forpredictingcyclotronbranchlesionbyleadⅠ , ⅢoflimbleadECG mainlyinanginaattack.
    Effect and hemodynamics of a low dose dexmedetomidine on ropivacaine subarachnoid block
    YANGGuang-yao,WANG Hong,SONGTie-ying,MAPeng-yu,GU Kun-feng
    2016, 37(1):  50-53.  doi:10.3969/j.issn.10073205.2016.01.014
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    ] Objective Tostudytheeffectandhemodynamicsofalowdosedexmedetomidine
    onropivacainesubarachnoidblock.Methods Fifty-twopatientswithlowerextremitysurgery
    weretreated by subarachnoid block anesthesia.The patients wererandomly dividedinto
    observationgroup ( n =26 ) andcontrolgroup (
    n =26 ) .Thepatientsinobservationgroupwere
    usedwithnarcoticdrugs3 μ gdextralmedetomidine+5g / Lropivacaine , whilethoseofcontrol
    groupweretreatedwithropivacaine5g
    / Lalone.Theeffectofanesthesia , adversereactionsand
    hemodynamicparameterswereobservedintwogroups.Results Theonsettimeofsensoryblock
    andtheonsettimeofmotorblockinobservationgroupweresignificantlyshorterthanthoseof
    controlgroup , but , thetimeofsensoryblockandexercisetimeinobservationgroup were
    significantlylongerthanthoseincontrolgroup ( P <0.01 ) .TheBromagescoreandcomfortscore
    inobservationgroupweresignificantlyhigherthanthoseincontrolgroup ( P >0.05 ) .Twogroups
    showednosignificantchangesinhemodynamicparameterssuchas MAP , SV , andCOafter
    beingtreated0 , 10 , 30 , 60 , 90minutes ( P >0.05 ) .Therewerenosignificantdifferenceinthe
    incidence of adverse reactions between two groups ( P > 0.05 ) .Conclusion Low dose
    dexmedetomidinemidazolamhasgoodeffectinropivacainesubarachnoidblock.Thedrugshows
    highclinicalapplicationvaluelessduetoadversereactionsandnosignificanteffectforblood
    dynamicindexes.
    Highfrequencyultrasoundobservationofmammaryductclosure#br# afterterminationofbreastfeedingandclinicalsignificance
    HUANG Lei, HUANGYin-ping 2*,ZHANGDan 3,LIU Qing-duo 4,ZHANGJin-chao 5
    2016, 37(1):  54-56,60.  doi:10.3969/j.issn.10073205.2016.01.015
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    ] Objective Todiscusstheclosureconditionandrulesofmammaryductbyhigh
    frequencyultrasoundobservationafterterminationofbreastfeeding , andprovideabasisfor
    identificationandclinicaldiagnosisandtreatmentofmammaryductdiseases.Methods High
    frequencyultrasoundwasusedtoobservethemammaryductclosurein428caseswithin1year
    aftertheterminationofbreastfeeding.Accordingtoterminatingbreastfeedingafter0-2
    months , 3-4months , 5-6months , 7-8months , 9-10months , 11-12months , 6hours ,
    thedudiscontinuenursingwasexplored.Results Theductclosurerateinsixdifferentperiodswere
    significantlydifferent ( P <0.01 ), and5-6 months , 7-8 months , 9-10 months , 11-12
    monthswerehigherthan0-2monthsand3-4monthsgroup ( P <0.05 ) .Theterminationof
    ductclosurerateraisedwithprolongedbreastfeeding (χ
    2 =54.580 ,
    P =0.000 ) .Oneyearfollow-
    upindicatedthatductclosureratewasstatisticallysignificantinthesixdifferentperiods ( P <
    0.01 ), and11-12monthsgroupwaslowerthan0-2months , 3-4monthsand5-6months
    (
    P <0.05 ) .Conclusion Aftertheterminationofbreastfeeding , mammaryductwasgradually
    closed.Within7 months , mammaryductclosurerateincreasedwiththeterminationtimeof
    lactation , after7 months , thebreastductclosureratehadnosignificantchange withthe
    terminationtimeoflactation.Therefore , moreobservationneedswhenthebreastisnotclosedat
    theterminationofbreastfeedingwithin7months.Morethan7monthswhenbreastductisnot
    closedwithsymptoms , timelytreatmentshouldbeinchoice.
    Determination of related substances in lansoprazole for injection by high performance liquid chromatography
    XU Meng1,LU Meng-xi 2,ZHOUChun-hua 1*,XUDe-hua3,ZHANGXue-ning 1,SONGJuan 1
    2016, 37(1):  57-60.  doi:10.3969/j.issn.10073205.2016.01.016
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     Objective Tousehighperformanceliquidchromatographyforthedetermination
    ofthecontentoflansoprazoleandlansoprazoleinjection.Methods Thedetermination was
    carriedoutonwithaDikmaDiamonsilTM2C 18 ( 4. 6mm×250mm , 5 μ m ) column.Themobile
    phasewasmethanol-water ( 70∶30 ) .Thedeterminationwavelengthwas285nm.Results The
    excipientsdidnotinterferewiththedeterminationoflansoprazole.Thedevelopedmethodcould
    beusedforthe determination oflansoprazoleanditsinjection.The calibration curve of
    lansoprazolewasingoodlinearityovertherangeof50-500mg
    / L.Theprecisionofthemethod
    (
    relativestandarddeviation [ RSD ] =0.14% ) wasgood.Theaveragerecoveryofthelow ,
    medium , andhighconcentrationswere100.0% , 98.3% , 101.7% with RSD0.5% , 0.3% ,
    0.4%respectively.Conclusion Themethodissimple , accurateandspecific.Itcanbeusedfor
    thedeterminationoflansoprazole.