Abstract:  Objective ToestablishamouseLeydigcell-Sertolicell-germcelldouble-chamber
coculturesysteminvitro withoutserum.Methods TheLeydigcellswereisolatedfromthe
testesofpostnatalday60maleC57BL / 6mice.TotalgermcellsandSertolicellswereisolated
fromthetestesofpostnatalday15maleC57BL / 6mice.Thecellsinbicameralchambersculture
system weregrowninmediawithoutserum.Themorphologyandgrowthofculturecellswere
monitoreddailyundercontrastphasemicroscope , andwereidentifiedbyHematoxylinstaining.
Ploidyanalysisofcellswasobservedbyflowcytometry.Results Inbicameralculturesystem ,
sporadicround spermatids were detected after one week , and elongating spermatids or
spermatidswithflagellawereobservedafterthreeweeks.Thespermatogeniccellssurvivedas
longaseightweeks.Thehaploidpeakwasdetectedafteroneweek ; thepercentageofmonoploid
increasedwithculturetime.Conclusion Thespermatogeniccellsinbicameralculturesystem
maturedintomorphologicallynormalspermatozoaandsurvivedlonger.

Key words: permatids , cellculturetechniques , mice