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Table of Content

    25 December 2019, Volume 40 Issue 1
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    Expression change of GPX1 in hepatic ischemiareperfusion injury rat model
    YU Guoxia1, YANG Junhui2, HUO Hongchang1, WANG Lei3, WANG Qie3*
    2019, 40(1):  7-10,15.  doi:10.3969/j.issn.10073205.2019.01.003
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    ［Abstract］ Objective〖HTSS〗〓To observe the expression change of glutathione peroxidase1(GPX1) in rats of hepatic ischemiareperfusion injury(HIRI) model and investigate the role of GPX1 in scavenging peroxide and reducing peroxidative injury induced by ischemia and reperfusion.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The HIRI model of rats was established by blocking the hepatic pedicle of the right and middle lobes for 30 mins. The blood and liver were taken after 6 hours of reperfusion. The activity of serum ALT was detected with rate method, the changes of liver morphology and structure in rats was observed by HE staining, the malondialdehyde（MDA） and hydrogen peroxide(H2O2 ) content in liver were determined by Thiobarbituric acid colorimetric method，and Molybdate colorimetric method respectively. The GPX1 mRNA and protein expression level were 〖JP2〗evaluated by RTPCR and Western blotting respectively, the correlation between the GPX1〖JP〗 expression level and the MDA content, between the GPX1 expression level and the H2O2 content were analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the rats of control group, the morphological structure of liver of HIRI group was significantly damaged, the serum ALT activity, the content of MDA and H2O2 in the liver of HIRI model were significantly increased, the mRNA and protein expression level of GPX1 in liver were also significantly enhanced, There were positive  correlation between GPX1 mRNA and MDA, 〖JP2〗between GPX1 pretein and MDA, between GPX1 mRNA and H2O2, between GPX1〖JP〗 pretein and H2O2.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Ischemia and reperfusion causes liver tissue to be in oxidative stress and been subjected to peroxidative damage. Antioxidant enzymes GPX1 may play an antioxidant stress role by scavenging peroxides during the process, and reduce hepatic peroxidative injury induced by ischemiareperfusion.
    The application of atorvastatin combined with clopidogrel in intravascular intervention of patients with extracranial carotid stenosis#br#
    ZHOU Weikun1, HUANG Genlin2, DENG Xinxin1, MO Junning2, LIN Peijian1, LI Fuxiang1
    2019, 40(1):  11-15.  doi:10.3969/j.issn.10073205.2019.01.004
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    ［Abstract］ Objective〖HTSS〗〓To investigate the application of atorvastatin combined with clopidogrel inintravascular interventionof patients with extracranial carotid stenosis(ECS).
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 82 patients with ECS who underwent electiveintravascular intervention in the department of neurology in the hospital were selected as study subjects, and they were randomly divided into the combined group(treated with atorvastatin combined with clopidogrel, n=41) and the control group(treated with clopidogrelafter operation, n=41). The curative effect and safety were compared between the two groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The successful rate of stent implantation in the two groups was 100%. The head and neck CT angiography showed no restenosis in the two groups in 3 months after operation. The scores of National Institutes of Health Stroke Scale, lowdensity lipoprotein cholesterol, total cholesterol, triglyceride, carotid artery intimamedia thickness, and the number and area of plaques were significantly decreased while MiniMental State Examination score and highdensity lipoprotein cholesterol were significantly increased in the two groups in 3 months after operation(P＜005). Changes of abovementioned indexes in the combined group were greater than those in the control group(P＜005). The platelet membrane glycoproteins(CD62p, CD63p, GP Ⅱb/Ⅲ a receptor complex) were significantly decreased in the two groups in 3 months after operation(P＜005)， but there was no significant difference between the two groups in platelet activation indexes(P＞005). There was no significant difference in the total incidence of complications between the two groups(P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Atorvastatin combined with clopidogrel can effectively improve the prognosis of ECS patients withintravascular intervention, improve blood lipid levels, relieve neurological impairment, improve cognitive function,and control platelet activation.
    Effects of atorvastatin and rosuvastatin on blood glucose in patients with nondiabetes but with cerebral infarction secondary prevention#br#
    CHEN Mei1, GUO Mingsheng2*, WU Hua3, LIU Juan2
    2019, 40(1):  16-19,24.  doi:10.3969/j.issn.10073205.2019.005
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    ［Abstract］ Objective〖HTSS〗〓To observe the level changes of blood glucose related indexes in patients with nondiabetes but with cerebral infarction secondary prevention, when they took different doses of atorvastatin or rosuvastatin to regulate blood lipids.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One  hundred and fifty patients were randomly divided into 5 groups, 30 patients in each group. They were respectively treated with 10 mg atorvastatin, 20 mg atorvastatin, 40 mg atorvastatin, 10 mg rosuvastatin or 20 mg rosuvastatin by oral  administration once a night. The course of treatment was 24 weeks. The level changes of fasting triglyceride(TG), total cholesterol(TC), low density lipoprotein cholesterol(LDLC), fasting blood glucose(FBG) and glycosylated hemoglobin(HbA1c) in those patients were observed before and after treatment.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓After treatment, the level of TG, TC and LDLC of each group were significantly decreased, HbA1c level of 20 mg atorvastatin group was significantly increased and the level of FBG and HbA1c of 40 mg atorvastatin group were significantly increased(P＜005). Pairwise comparison among groups after treatment: the level of TG, TC and LDLC of 10 mg atorvastatin group were significantly higher than those of other four groups, those of 40 mg atorvastatin group were significantly lower than those of 20 mg atorvastatin group and two rosuvastatin groups. Those of 20 mg rosuvastatin group were significantly lower than those of 10 mg rosuvastatin group(P＜005). HbA1c level of 40 mg atorvastatin group were significantly higher than that of other four groups(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The effect of rosuvastatin on FBG and HbA1c is less than that of atorvastatin when the lipidlowering effect of rosuvastatin is the same as that of atorvastatin.
    The comparison between abdominal obesity measured by CT and simple body fat parameters for the evaluation of insulin resistance#br#
    WANG Yuanyuan1, WANG Zhanjian2, LIU Kuanzhi2, WANG Shuchang2*
    2019, 40(1):  20-24.  doi:10.3969/j.issn.10073205.2019.01.006
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    ［Abstract］〓Objective〖HTSS〗〓To investigate the effects for evaluating insulin resistance (IR) by abdominal obesity measured by CT or through simple body fat parameters.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fifty patients with abdominal obesity and with normal glucose tolerance(Control group) and fifty patients with type 2 diabetes mellitus and abdominal obesity(T2DM group) were recruited. Waistline, hipline, body mass index(BMI), fasting blood glucose(FBG), fasting insulin(FINS), fasting Cpeptide, triglycerides(TG), highdensity lipoprotein cholesterol(HDLC), lowdensity lipoprotein cholesterol(LDLC), HOMA1insulin resistance index(HOMA1IRI), the area of intraabdominal adipose(I), the area of subcutaneous adipose(S), I/S ratio were detected. The correlation between HOMA1IRI and the other indexes above were analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓FBG, FINS, fasting Cpeptide, HOMA1IRI, lnHOMA1IRI, the area of intraabdominal adipose, I/S ratio were higher in T2DM group than that in the control group,  HDLC was much lower in T2DM group than that in the control group(P＜005). There was no significant difference in LDLC and the ratio of waistline and hipline between the two groups. There was a significantly positive correlation between lnHOMA1IRI and FBG, FINS, fasting Cpeptide, LDLC, TG, the area of intraabdominal adipose, I/S ratio, and HOMA1IRI. And there was a significantly negative correlation between lnHOMA1IRI and HDLC.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Abdominal obesity measured by CT was more sensitive than simple body fat parameters to evaluate IR.
    The clinical efficacy of levothyroxine and growth hormone in the treatment of dwarfism caused by hypothyroidism
    WU Tao, DU Junwen*, ZHANG Kun, REN Qiaohua, WANG Xin, LIANG Yi
    2019, 40(1):  25-28.  doi:10.3969/j.issn.10073205.2019.01.007
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    ［Abstract］〓Objective〖HTSS〗〓To study the exact effect of levothyroxine and growth hormone in the treatment of dwarfism caused by hypothyroidism.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Eighty children with short stature caused by hypothyroidism were selected and randomly divided into control group(levothyroxine tablet treatment) and experimental group(levothyroxine tablet + growth hormone treatment) according to different treatment methods, 40 cases in each group. Growth velocity(GV), height standard deviation score(HtSDS), predicted adult height(PAH), bone age(BA) and serum osteocalcin(OC) levels were compared before and after treatment for 3 and 6 months. The changes of serum thyroxine(T4) levels were recorded before and after treatment.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The levels of GV, HtSDS, PAH and OC increased with time after treatment. There was no significant difference in GV, HtSDS, PAH and OC levels at 3 months of treatment. At 6 months of treatment, GV, HtSDS, PAH and OC levels in the experimental group were significantly higher than those in the control group. There were significant differences in the interactions among groups, time points, and between groups（P＜005）. There was no significant difference in the interaction between BA groups, time points and grouptime point interaction P＞005). T4 levels in both groups increased significantly after 3 months of treatment(P＜005). However, there was no significant difference in the interaction between T4 groups, between groups and between time points after treatment P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Levothyroxine combined with recombinant human growth hormone in the treatment of dwarfism caused by hypothyroidism can effectively promote the growth of height, and significantly increase the level of thyroid hormone.
    The comparison of the effects and renal function of indapamide and losartan potassium hydrochlorothiazide tablets combined with nifedipine on the treatment of refractory simple systolic hypertension in elderly patients#br#
    LI Chunling， LI Guiping， LIN Junyu
    2019, 40(1):  29-32,37.  doi:10.3969/j.issn.10073205.2019.01.008
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    ［Abstract］ Objective〖HTSS〗〓To compare the effects and renal function of indapamide and losartan potassium hydrochlorothiazide tablets combined with nifedipine on the treatment of refractory simple systolic hypertension in elderly patients.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 100 patients with refractory simple systolic hypertension were enrolled. They were divided into study group and control group according to the random number table. The study group were given losartan potassium hydrochlorothiazide tablets combined with nifedipine controlled release tablets, the control group were given indapamide combined with nifedipine controlled release tablets treatment. The blood pressure, 24 h ambulatory blood pressure and heart rate, renal function, and the clinical efficacy of the two groups of patients were measured and observed before and after treatment.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓There were significant differences in the blood pressure between two groups, time points and twoway interaction at 1, 2 and 4 months after treatment(P＜005). After treatment, the 24hour mean systolic blood pressure(24 hSBP), 24hour mean diastolic blood pressure(24 hdSBP), daytime mean systolic blood pressure(dSBP), nighttime mean systolic blood pressure(nSBP) of the study group were significantly higher than those in the control group(P＜005). There was not significant difference in diastolic blood pressure and heart rate between two groups(P＞005). After treatment, the patients in the study group were significantly higher than those in the control group(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Losartan potassium hydrochlorothiazide tablets combined with nifedipine controlled release tablets to improve the elderly patients with intractable systolic hypertension than indapamide combined with nifedipine sustainedrelease tablets program is effective, the patients′ renal function are improved significantly.
    Efficacy and safety of alogliptin for patients with type 2 diabetic mild cognitive impairment#br#
    WANG Jing1， LIU Fan2*, DONG Shanshan2, FENG Hongyun2, YANG Aige2, ZHOU Huimin2
    2019, 40(1):  33-37.  doi:10.3969/j.issn.10073205.2019.01.009
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    ［Abstract］ Objective〖HTSS〗〓To explore the efficacy and safety of alogliptin, acarbose and repaglinide in patients with type 2 diabetic mild cognitive impairment inadequately controlled by metformin.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The 120 patients with type 2 diabetic mild cognitive impairment inadequately controlled by metformin were randomly divided into thealogliptin group(n=40), theacarbose group(n=40) and therepaglinidegroup(n=40). During the 22week treatment period, patients in the alogliptin group were given metformin and alogliptin, patients in the acarbose group were given metformin and acarbose and patients in the repaglinide group were given metformin and repaglinide. At week 0, 12 and 24 of the clinic trial, hemoglobin A1c(HbA1c), fasting plasma glucose(FPG), 2 h postprandial blood glucose(2 hPBG), triglycerides(TG), total cholesterol(TC) were measured, all hypoglycemic events and gastrointestinal reaction during the trial recorded and the percentage of patients achieving HbA1c target of <7% were recorded.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓After the treatment, HbA1c, FPG and 2 hPBG in three groups lowered obviously. Comparing with those before treatment, the differences were significant(P＜001), while there were no significant changes among the three groups in TG and TC（P＞005）. There were no significant difference among the three groups in HbA1c, FPG, 2 hPBG , TG and TC（P＞005）. After the treatment, the percentage of patients achieving HbA1c target of <7% was highest(74.36%, χ2=6.496， P＜001),  the incidence of hypoglycemia(2.56%， χ2=16.955， P＜001) and gastrointestinal reaction(0.00%, χ2=37.853， P＜001) in the alogliptin group was lowest  among the three groups.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The efficacy of alogliptin was not inferior to that of acarbose and regrainidein patients with type 2 diabetic mild cognitive impairment inadequately controlled by metformin, while compared with them, the incidences of hypoglycemia and gastrointestinal reaction were lowest in those treated by alogliptin.
    Effects of peritoneal dialysis and hemodialysis on the heart structure and function of patients with endstage renal disease
    ZHUO Jianqin
    2019, 40(1):  38-42.  doi:10.3969/j.issn.10073205.2019.01.010
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    ［Abstract］ Objective〖HTSS〗〓To observe the effect of peritoneal dialysis and hemodialysis on the heart structure and function of the patients with endstage nephropathy, and to investigate the correlation between clinical indicators and the structure and function of the heart.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred and eighty patients with endstage renal disease were divided into 90 cases of peritoneal dialysis group and 90 cases of hemodialysis group according to different dialysis methods. The residual urine volume, blood pressure, blood lipids and blood biochemical parameters of the two groups were compared. All patients underwent cardiac color ultrasonography before and after dialysis, and interventricular septum thickness(IVST), left ventricular pesterior wall diastolic thickness(LVPWDT), left ventricular end diastolic diameter left ventricular enddiastolic diameter(LVEDD), left atrium diameter(LAAP), left ventricular ejection fraction(LVEF), and left ventricular myocardial mass index left ventricular myocardial mass index(LVMI) of two groups were observed. The relationships between above observations and LVMI were analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓After dialysis, the residual urine volume and total cholesterol in hemodialysis group were less than that in the peritoneal dialysis group. IVST, LVPWDT, LVEDD, LAAP, and LVMI in hemodialysis group were larger than the peritoneal dialysis group, differences were statistically significant(P＜005). The mean arterial pressure in the peritoneal dialysis group was significantly higher than that in the hemodialysis group, while the blood albumin content was decreased, with statistically significant differences(P＜005).The residual urine volume and mean arterial pressure were significantly different in the peritoneal dialysis group(P＜005). In patients with endstage renal disease, there was a positive correlation between LVMI and systolic pressure(r=0.379, P＜005), and a negative correlation between residual urine, hemoglobin and low density lipoprotein cholesterol(r=-0.423, -0.342, -0.289, P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Hemodialysis has a greater impact on cardiac structure and function in patients with endstage renal disease than peritoneal dialysis, Peritoneal dialysis may be a more ideal method for patients with endstage renal disease.
    A study on the changes of endothelial glycocalyx in rabbits with septic shock induced by different factors#br#
    WU Xinhui1, HU Zhenjie1*, CHEN Lei1, LI Yong2, ZHANG Tao1, HAN Yaqi1
    2019, 40(1):  43-47,51.  doi:10.3969/j.issn.10073205.2019.01.011
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    ［Abstract］ Objective〖HTSS〗〓To observe the alterations of endothelial glycocalyx in early septic shock rabbits induced by different pathogenic factors.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirtysix male New Zealand rabbits were divided into puncture cecal ligation and puncture(CLP) group, the CLP control group, the CLP 30 mL group, the lipopolysaccharide(LPS) group, the LPS control group and the LPS 30 mL group, 6 rabbits in each group according to a random number table. After establishment of rabbit model of LPS and CLP induced septic shock, they were managed accordingly and the clinical indicators and time for model establishment of each group were recorded and compared.Concentrations of syndecan1 in plasma were measured by enzyme linked immunosorbent assay at different time points early after septic model was established. After six hours, the rabbits were sacrificed, and various organs and tissues were harvested. Western blot and immunohistochemistry were used to detect the expression of endothelial glycocalyx.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The modeling time of CLP group and LPS group was significantly different(P＜005). There were no statistical differencesin white blood cell count and lactic acid between CLP group and LPS group(P＞005), as well as between CLP control group and LPS control group(P＞005). However, significant differences were found between model group and control group. In addition, model group and control group showed significant differences in central venous blood oxygen saturation(P＜005), as well as between LPS group and CLP group(P＜005). Results of plasma markers revealed that the concentration of plasma syndecan1 was significantly higher in CLP group than in LPS group. HE staining revealed less renal damage in LPS group than in CLP group. Immunohistochemical results showed that the expression of syndencan1 which was darker and higher in LPS group than in CLP group. Western blot results indicated that the expressions of syndecan1 in renal tissues in LPS groups were higher than that in CLP groups.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The endothelial glycocalyx was damaged by both LPS and CLP.LPS caused less damage to endothelial glycocalyx than CLP. After fluid resuscitationcombined with norepinephrine, endothelial glycocalyx will be more likely to be partially restored in LPS group in a short term.
    Application of nonuniform settlement theory in spine degenerative kyphosis#br#
    WEI Jianlu1,2，WANG Juan2，CHEN Wei1，WANG Hong3，CHENG Lei2，ZHANG Yingze1*
    2019, 40(1):  48-51.  doi:10.3969/j.issn.10073205.2019.01.012
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    ［Abstract］〓Objective〖HTSS〗〓To examine the change of the ratio of anterior and posterior column and pedicle angle in lumbar vertebrae by using nonuniform settlement theory.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Twenty osteoporosis patients and 20 nonosteoporosis patients were selected for computed tomography（CT） examination. The ratio of anterior and posterior column of thoracic vertebra 12(T12) to lumbar vertebra 2(L2) was measured on sagittal CT reconstruction. Magerl needle insertion point was selected on CT cross view to measure the angle of pedicle angle.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The anterior/posterior ratio of T12-L2 vertebral body in osteoporosis group was lower than that in nonosteoporosis group, and the pedicle angle of L1-L2 vertebral body was lower than that in nonosteoporosis group(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓In patients with osteoporosis, the anterior column height of the lumbar spine was decreased and the ratio of anterior and posterior column of the vertebral body was decreased. The pedicle angle of patients with osteoporosis is smaller than that of nonosteoporosis patients. Clinicians should appropriately reduce the angle of pedicle insertion in the operation of osteoporosis patients.
    The expression and clinical significance of Gas6 and MMP9 in cholangiocarcinoma#br#
    WANG Ziwei1, WU Juan2, FENG Hao1
    2019, 40(1):  52-55.  doi:10.3969/j.issn.10073205.2019.01.013
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    ［Abstract］〓Objective〖HTSS〗〓To study the expression of growth arrest specific6(Gas6) and matrix metallo proteinase9（MMP9） in cholangiocarcinoma and its correlation with clinical parameters.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The expression of Gas6 and MMP9 protein was detected by immunohistochemistry(SP) in 60 cases of cholangiocarcinoma and 17 cases of paracancerous bile ducts, combined with the clinical parameters of patients(age, tumor differentiation, tumor size, with or without lymph node metastasis, with or without hepatic artery and portal vein invasion) and other clinical factors for comprehensive analysis.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The positive expression rate of Gas6 in cholangiocarcinoma was 71.7%, which was higher than that in paracancerous tissues(29.4%)(P＜005). The positive expression rate of Gas6 in cholangiocarcinoma was correlated with lymph node metastasis and hepatic artery or portal vein invasion(P＜005). The positive expression rate of MMP9 in cholangiocarcinoma was 66.7%, which was higher than that in paracancerous tissues(35.3%)(P＜005). The expression of MMP9 in cholangiocarcinoma was correlated with lymph node metastasis and hepatic artery or portal vein invasion(P＜005). There was positive relationship between the expression of Gas6 and MMP9(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The expression of Gas6 and MMP9 was closly associated with the development and progression of cholangiocarcinoma.
    Risk factors of postoperative systemic inflammatory response syndrome in elderly patients with upper urinary tract calculi treated by percutaneous nephrolithotomy#br#
    SHI Jianguo, WANG Weining*, WANG Lingjun， CHEN Yudong, HAN Gang
    2019, 40(1):  56-59.  doi:10.3969/j.issn.10073205.2019.01.014
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    ［Abstract］ Objective〖HTSS〗〓To investigate the risk factors of systemic inflammatory response syndrome(SIRS) in elderly patients with upper urinary calculi treated by percutaneous nephrolithotomy.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A retrospective analysis of 95 elderly patients with upper urinary calculi after percutaneous nephrolithotomy was performed to evaluate the correlation between the various factors and the postoperative SIRS.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Of the 95 patients, 22(23.2%) had SIRS, of which 2 had septic shock. After active antiinfection and supportive treatment, the patients recovered without death. History of diabetes mellitus, repeated urinary tract infection, positive rate of preoperative urine culture, the rate of staghorn calculi, stone load and operation time in SIRS group were significantly higher than those in nonSIRS group, and the difference was statistically significant. Logistic regression analysis showed that history of recurrent urinary tract infection, staghorn calculi, and diabetes mellitus were risk factors for SIRS in elderly patients after percutaneous nephroscopy.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓For elderly patients with history of recurrent urinary tract infection, staghorn calculi and diabetes mellitus, vigilance should be raised, infection should be actively controlled before operation, renal collecting system damage should be avoided during operation, and strict monitoring after operation should be done to reduce the incidence of SIRS.
    Effects of intestinal stents implantation combined with neoadjuvant chemotherapy on surgical operation and malignant biological behavior of patients with obstructive left hemicolon carcinoma#br#
    LI Xubin, JIA Junfeng*, ZHANG Qijun, SHAN Haifeng, ZHANG Jie
    2019, 40(1):  60-64,69.  doi:10.3969/j.issn.10073205.2019.01.015
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    〗［Abstract］〓Objective〖HTSS〗〓To analyze the effect of stent implantation combined with neoadjuvant chemotherapy on the surgical outcome and malignant biological behavior of the patients with obstructive leftsided obstructing colon cancer.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The clinical data of 81 patients with acute intestinal obstruction caused by left colon cancer were retrospectively analyzed. According to the different treatment methods，there were 42 cases in the control group and 39 patients in the experimental group. The control group underwent radical open colon resection for colon cancer. The experimental group were received the chemotherapy after the stent was implanted, and the delayed radical colon surgery .The serum samples were taken to detect the level of tumor markers, and tumor tissues were taken to detect the expression of malignant molecules. The blood loss during operation, the number of lymph nodes, the time from operation to exhaust, the incidence of intestinal anastomosis leakage the condition of stage one resection and complete anastomosis, and the infection rate of incision were compared between the two groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The levels of serum carcinoembryonic antigen(CEA), CA724, CA199 and CA125 in the control group were significantly lower than those before the operation(P＜005). The levels of serum CEA, CA724, CA199 and CA125 in the experimental group were significantly lower than those before the operation, the levels of serum CEA, CA724, CA199 and CA125 were significantly lower than those after chemotherapy(P＜005). The levels of serum CEA, CA724, CA199 and CA125 in the experimental group were significantly lower than those in the control group(P＜005). After surgical resection, the mRNA expression levels of the SMYD3, CHD1L， osteopontin and Survivin in the experimental group was significantly lower than those in the control group(P＜005). In the experimental group, the time of operation, the amount of blood lost during operation, the incidence of anastomotic leakage, and the incidence of incision infection were significantly lower than those in the control group， the rate of one stage anastomosis after resection was significantly higher than that of control group(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Obstructive left colon cancer with stent implanted to relieve the obstruction and followed by neoadjuvant chemotherapy and then radical mastectomy can effectively reduce the biological malignancy of tumor tissue and inhibit the malignant molecular expression of malignant biological behavior, reduce tumor grading and staging, and effectively avoid emergency surgery, thus reduce the risk of surgery, increase the surgical effect, and eventually benefiting.
    Comparison of orthodontic treatment between malocclusion and traditional fixed orthodontics in children with malocclusion
    ZHAI Mingbiao, SUN Yan, GUO Jianhong, MA Shuze
    2019, 40(1):  65-69.  doi:10.3969/j.issn.10073205.2019.01.016
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    ［Abstract］〓Objective〖HTSS〗〓To explore the effect of invisible brackets in orthodontic treatment of children with malocclusion.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Eightytwo children with malocclusion who needed orthodontic treatment were divided into observation group(39 cases) and control group(43 cases) according to different treatment methods. The observation group was treated with invisible correction without bracket, and the control group was treated with traditional fixation for 6 months. The clinical effects of the two groups were compared. The periodontal index(GI), sulcus probing depth(SPD), sulcus bleeding index(SBI) and plaque index(PLI) of the two groups were compared before and after treatment. The orthodontic time of inclined teeth, orthodontic time of transposition teeth and the end of treatment were compared. The time needed for completion, the satisfaction after correction, and the incidence of adverse reactions during treatmentwere also compared.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The clinical efficacy of the observation group was better than that of the control group, and the total effective rate was higher than that of the control group(P＜005). After treatment, the levels of PLI and SBI in the control group were significantly higher than those before treatment, while those in the observation group were significantly lower than those in the control group(P＜005). The righting time of the tilted teeth and the time of twisting teeth were significantly shorter in the observation group than in the control group(P＜005). The satisfaction scores of aesthetic comfort, retention function, masticatory function and language function in the observation group were significantly higher than those in the control group(P＜005). The incidence of oral mucosal injury, caries and gingivitis in the observation group was significantly lower than that in the control group(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Stealth orthodontic treatment without bracket has a good effect in the orthodontic treatment of children with malocclusion. It is helpful to maintain oral hygiene, does not affect periodontal health, and can improve patient satisfaction, reduce the occurrence of oral adverse reactions during treatment. It is worth popularizing and applying.
    Effect of cyclic loading on the compressive strength of fullcontour zirconia crowns
    WU Shuo, LIAN Yunmin*, GAO Lan, WANG Pu
    2019, 40(1):  70-73.  doi:10.3969/j.issn.10073205.2019.01.017
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    〗［Abstract］ Objective〖HTSS〗〓To evaluate and compare the resistance level of fullcontour zirconia crowns under cyclic loading.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A computer aided design and computer aided manufacturing(CAD/CAM) system was used to prepare 18 fullcontour zirconia crowns of the mandible first molar. All the crowns were bonded to the metal dies using 3M glass ionomer cement and were randomly divided into A, B, C groups, each group with six. Group A and group B was respectively fixed on the biomechanical test machine for cyclic loading, with a frequency of 8 Hz, 300 000 cycles.The loading force of group A was 300 N,and group B was 500 N. The compressive strength were measured by the universal testing machine. Used SPSS 21.0 software to analyze the data.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓There were no statistical significant differences between two groups(P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Using different force  for 300 000 cycles on the zirconium crown load had no significant effect on its resistance to compressive strength.
    Application of behavioral management in dental operative treatment of children with hematological diseases
    DU Ning1, ZHANG Zhe2, ZHOU Xiang3, LIU Xuecong1*
    2019, 40(1):  74-77,81.  doi:10.3969/j.issn.10073205.2019.01.018
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    ［Abstract］〓Objective〖HTSS〗〓To summarize the experience of clinical treatment of dental diseases in recent years in children with hematologic diseases, to investigate the effectiveness of behavior management in the treatment of dental diseases in children with hematologic diseases.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓In oral clinic, 46 children aged 3 to 7 years with hematological diseases were randomly divided into two groups: the experimental group(n=23) and the control group(n=23). The experimental group used behavioral management methods such as language communication, observation and learning, and physical restriction in clinical treatment, while the control group was treated only with routine operation. The coordination behaviors of 2 groups of children were evaluated and analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The cooperation of dental operation in the experimental group was better than that in the control group, the time of dental operation in the experimental group was shorter than that in the control group, and the receiving behavior of the children in the experimental group was better than that in the control group. The difference was statistically significant(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Children with blood system diseases have more times of going to medical treatment, have a sense of fear about the medical institutions themselves. The correct induction of behavior management in the clinical treatment of dental diseases can reduce the fear of children, increase the trust of medical staff, and promote the successful completion of dental treatment and reduce the oral infection rate, thereby alleviate the pain of children.
    The application effect of bioceramic iroot bp PLUS on patients with localized acute pulpitis undergoing vital pulp preservation#br#
    YANG Guang1, YIN Xuelian1, MA Dongjie2, ZHANG Hailong1, SU Zhejun1, HUO Feng1*
    2019, 40(1):  78-81.  doi:10.3969/j.issn.10073205.2019.01.019
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    ［Abstract］〓Objective〖HTSS〗〓To investigate the application effect of bioceramic iroot bp PLUS on patients with localized acute pulpitis undergoing vital pulp preservation.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 192 offending teeth of 110 patients with pulpitis were selected. According to the simple random method, they were divided into observation group and control group, 96 in each group. The control group was coated with calcium hydroxide paste, and the observation group was treated with bioceramic iroot bp PLUS for coronary resection.The efficacy, the restoration of the affected tooth and satisfaction of the two groups were observed and compared.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The clinical efficacy of the observation group was better than that of the control group on the first day and the seventh day(P＜005). There was no significant difference in the clinical efficacy between the two groups at 3 months(P＞005).The pain level of further consultation of the observation group was significantly better than that of the control group(P＜005). The gingival condition, secondary caries and tooth fracture in the observation group were better than in the control group(P＜005). There was no significant difference in edge staining, marginal integrity and prosthesis fracture between the two groups(P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The bioceramic iroot bp PLUS has better filling effect in patients with localized acute pulpitis who undergoing pulp preservation, and the tooth defect can achieve satisfactory repair effect and the pain recovery is better, which is worthy of clinical promotion.
    A retrospective clinical study of risk factors associated with maxillofacial multiple space infections
    SONG Donghui， WU Senbin， ZHU Jiang
    2019, 40(1):  82-85,90.  doi:10.3969/j.issn.10073205.2019.01.020
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    ［Abstract］ Objective〖HTSS〗〓To explore and analyze the risk factors associated with maxillofacial multiple space infections.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 115 patients diagnosed with oral and maxillofacial multiple space infection in our hospital were selected as the research subjects, and the clinical data of those patients was retrospectively analyzed. The etiological factors, clinical presentations, laboratory tests and complications were recorded. At the same time, single factor test and Logistic regression analysis were performed to evaluate the factors that caused the prolongation of hospitalization and complications.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Incidence and etiological classification: odontogenic 41/115(35.65%), lymphadenitis 27/115(23.47%), sialadenitis 16/115(13.91%), cyst 16/115(13.91%), trauma and unknown reason 15/115(13.04%), of which odontogenic infection is the most common cause. The number of spaces involved, the number of leukocytes, the blood glucose concentration, the percentage of neutrophils of patients hospitalized≥ 15 days were different of  those of patients hospitalized＜15 days, and the differences were statistically significant(P＜005). The age, associated underlying systemic diseases, the number of spaces involved, the white blood cell count, the blood glucose concentration, percentage of granulocytes  in patients with oral and maxillofacial multiple space infection combined with complications were different of those in patients without complications, and the differences were statistically significant(P＜005). Logistic regression analysis showed that the hospitalization time in patients with oral and maxillofacial multiple space infection was related to the number of spaces involved, white blood cell count, blood glucose concentration and neutrophil percentage. The complications of patients with oral and maxillofacial multiple space infections were related to the number of spaces involved, white blood cell counts, blood glucose levels, neutrophil percentage, and age.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Risk factors associated with hospitalization time and complications in oral and maxillo facial multiple space infections patients included associated underlying systemic diseases, the number of spaces involved, white blood cell count, blood glucose concentration, neutrophil percentage, and age, all the risk factors above were closely related to the prognosis of the disease, Those patients are at higher risk of developing lifethreatening complications and require early adequate treatment of their conditions and close clinical monitoring.
    Effect of aquatic therapy in patients of peroneal nerve injury#br#
    WANG Fei, WANG Yizhao*, ZHAO Hua, YIN Miaomiao, DONG Na, ZHANG Yue
    2019, 40(1):  86-90.  doi:10.3969/j.issn.10073205.2019.01.021
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    ［Abstract］ Objective〖HTSS〗〓To investigate the walking ability and the recovery of common peroneal nerve after hydrotherapy in patients with common peroneal nerve injury.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Twenty patients with common peroneal nerve injury were randomly divided into 10 cases in control group and 10 cases in experimental group . The control group only performed routine rehabilitation training; the experimental group added water exercise training in addition to the routine training. The total training duration of the two groups was 40 min(40 min field training for the control group and 20 min field training, 20 min water training for the experimental group), with 5 times a week for 8 weeks. After training, the two groups of calf circumference, dorsiflexion active joint mobility, 10 m walking speed, and nerve fiber conduction velocity and amplitude were compared.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓There was no significant difference in the calf circumference between two groups after treatment comparing before the training(P＞005). There was no difference in the calf circumference between two groups after treatment. After treatment, the active anterior flexion of the dorsiflexion and the walking speed of 10 m were better than those before treatment. The difference was statistically significant(P＜005), but the increase was greater in the experimental group. The active joint mobility and 10meter walking speed of the dorsiflexion were more than that of the control group after treatment(P＜005). After treatment, the motor nerve conduction velocity and amplitude were higher in the experimental group than before treatment(P＜005). After the treatment, the total nerve motor nerve conduction velocity was higher than that before treatment, and the amplitude was not significantly different from that before treatment(P＞0.05). After treatment, the conduction velocity and amplitude of the experimental group were higher than the control group, and the difference was statistically significant(P＜005). After treatment, the sensory nerve conduction velocity and amplitude of the experimental group were higher than before treatment(P＜005). The conduction rate of the control group was higher than that before treatment. The experimental group was higher than the control group after treatment, and the amplitude of the control group after treatment was compared with that before treatment. The difference was not statistically significant(P＞005). There was no significant difference between the experimental group and the control group after treatment(P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Eight weeks training in water can effectively improve patients nerve recovery, and helping patients improve their walking ability.
    Determination of 12 kinds of compounds in Baipuhuang capsules by UPLCMS/MS#br#
    WEI Tingting, JIN Miaomiao, GAN Xiaoruo, MA Menghan, YANG Guozhen, XU Huijun*
    2019, 40(1):  91-95.  doi:10.3969/j.issn.10073205.2019.01.022
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    ［Abstract］ Objective〖HTSS〗〓To develop a ultra high performance liquid chromatography tandem mass spectrometry(UPLCESIMS/MS) method for the simultaneous determination of 12 compounds(βecdysterone, ajugasterone C, hederasaponin C, cirenshenoside S, pulsatilloside C, C47H76O17, anemoside B4, hederasaponin B, anemoside A3, αhederin, 23hydroxybetulinic acid and hederagenin) in Baipuhuang capsules.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The chromatographic separation was performed on a Phenomenex KinetexC18 column (100×3 mm, 2.6 mm) and the column temperature was set at 40 ℃. The mobile phase, 0.1%(v/v) formic acid (A) and methanol consisted of 0.1% formic acid (B), were run at a flow rate of 0.4 mL/min and the analytes were eluted with a gradient system. Multiplereaction monitoring scanning was employed for determination with positive and negative modes. The conditions of MS/MS detector were set as follows: source temperature 150 ℃, capillary voltage 2.0 kV, and desolvation temperature 350 ℃, desolvation and cone gas at a flow rate of 800 and 150 L/h, respectively. Atomization gas pressure 7.0 Bar and the interface heater was turned on.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The complete separation was obtained within 14 min for the 12 compounds(βecdysterone, ajugasterone C, hederasaponin C, cirenshenoside S, pulsatilloside C, C47H76O17, anemoside B4, hederasaponin B, anemoside A3, αhederin, 23hydroxybetulinic acid and hederagenin). Seven regression equations showing linear relationships between peak area and content of each compound were obtained. The recoveries(n=6) of the compounds were 97.13%-101.2% and relative standard deviation were 1.2%-4.1%, respectively.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The analytical method established in this study posseses the advantage of simple operation, rapid speed, strong specificity and high sensitivity, and can be employed to the simultaneous quantification of multiple components in Baipuhuang capsules.
    Law of dynamic deformation of bone
    CHEN Wei, HOU Zhiyong, LYU Hongzhi, WEI Jianlu, WANG Juan, YE Dandan, ZHANG Yingze*
    2019, 40(1):  115-116.  doi:10.3969/j.issn.10073205.2019.01.029
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    ［Abstract］〖HTSS〗〓The bone in living body are dynamically changing. The shape of bone, such as diameter, length, curve and so on, can be changed accordingly under stress. This kind of change of shape are regular, which is named as Zhang′s law of dynamic deformation of bone. Our team has conducted a series of studies on the dynamic deformation of bone. Based upon these studies, the laws of dynamic deformation of each bone has been described, and the dynamic deformation of bone has also been found to be highly correlated with degenerative disease. The dynamic deformation of bone is an important sign of the development of degenerative disease, which plays an important role in the selection of best treatment algorithm.