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Table of Content

    25 January 2020, Volume 41 Issue 1
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    Overexpression of miR-140-5p promotes apoptosis and inhibits proliferation of prostate cancer cells#br#
    PANG Ya-xiang1, HAN Hua2, YIN Yue1, MA Guo-hua3, NIU Hong-lin4*
    2020, 41(1):  7-11.  doi:10.3969/j.issn.1007-3205.2020.01.002
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    ［Abstract］Objective〖HTSS〗To investigate the expression of miR-140-5p in prostate cancer cells and explore the biological functions of miR-140-5p in prostate cancer progression.
    Methods〖HTSS〗RT-qPCR was performed to identify the expression of miR-140-5p in PC3 and DU145 cell lines. CCK8 assay and AnnexinV-FITC/PI stain were used to exam cell proliferation cell apoptosis. TCGA database was used to analyze the relationship between miR-140-5p expression level and survival of prostate cancer patient.
    Results〖HTSS〗miR-140-5p was downregulated in PC3 and DU145 cells.Transfection of miR-140-5p mimic to PC3 cells significantly increased miR-140-5p level in PC3 cells. Overexpression of miR-140-5p in PC3 cells increased the cell apoptosis and decreased cell viability. Transfection of miR-140-5p inhibitor to PC3 cells significantly decreased miR-140-5p level in PC3 cells. Knockdown of miR-140-5p in PC3 cells decreased cell apoptosis and increased cell viability. Lower miR-140-5p level of prostate cancer patients had a poor overall survival analyzing from TCGA database.
    Conclusion〖HTSS〗miR-140-5p is downregulated in prostate cell lines and correlates with poor survival of prostate cancer. Overexpression of miR-140-5p in PC3 cells promotes cell apoptosis and inhibits cell growth.
    Protective effects of dl-3-n-butyphthalide on angiogenesis in myocardial infarction rats
    TIAN Xiao-chao1, HE Wei-liang2, JIA Yan1, WU Yu-zhou1, JIN Xin1, LIU Su-yun1*
    2020, 41(1):  12-16.  doi:10.3969/j.issn.1007-3205.2020.01.003
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    ［Abstract］Objective  To study protective effects of dl-3-n-butyphthalide(NBP) on angiogenesis in rats with myocardial infarction and explore its related mechanism.
    Methods  Myocardial infarction model in SD rats was used. The rats were divided into sham operation group, myocardial infarction(MI) group, the NBP low-dose group(20 mg/kg) and the NBP high-dose group(40 mg/kg). There were 6 rats in each group. The NBP was injected to rats once a day for 2 weeks. 2, 3, 5-Triphenyltetrazolium chloride staining was used to detect myocardial infarction areas. The cardiac functions were evaluated using echocardiography. New blood capillaries density in myocardial infarction fringe area was measured by immunohistochemical analysis. The gene expressions of vascular growth factor(VEGF), basic fibroblast growth factor(β-FGF), and Notch 1 were determined by RT-qPCR.
    Results〖HTSS〗No myocardial infarction was found in the Sham group. The percentage of myocardial infarction area in MI group, NBP-L group and NBP-H group was higher than Sham group. However, it was lower in NBP-L group and NBP-H group than that in MI group, and it was lower in NBP-H group than that in NBP-L group(P＜0.05). LVESP, +dp/dt Max and -dp/dt Max of rats in the MI, NBP-L and NBP-H groups were lower than those in the Sham group, while LVED was higher than those in the Sham group. LVESP, +dp/dt Max and -dp/dt Max of rats in the NBP-L group and NBP-H group were higher than those in the MI group, while LVED was lower than those in the MI group. LVESP, +dp/dt Max and -dp/dt Max of rats in the NBP-H group were higher than those in the NBP-L group, and LVED in the NBP-H group was lower than those in the NBP-L group(P＜0.05). The capillary density in the edge of myocardial infarction in NBP-L group and NBP-H group was higher than that in MI group, and it was higher in NBP-H group than that in NBP-L group(P＜0.05).The mRNA level of VEGF and β-FGF in MI group was lower than that in Sham group, however, in NBP-L group and NBP-H group was higher than that in Sham group and MI group, and the mRNA level of VEGF and β-FGF in NBP-H group was higher than that in NBP-L group(P＜0.05). Notch 1 gene expression in the myocardial tissue of rats in the MI group was significantly lower than that in the Sham group. However, in the NBP-L group and the NBP-H group was significantly higher than that in the Sham group and the MI group. Notch 1 gene expression in the NBP-H group was significantly higher than that in the NBP-L group(P＜0.05).
    Conclusion〖HTSS〗NBP obviously promotes angiogenesis in myocardial infarction rats, which may related to activation of Notch 1 pathway.
    Application of CTA and cervical vascular color Dopplerultrasound in diagnosis of extracranial lesions of carotid artery in ACI patients#br#
    LI Qing1, NI Jian-qiang2, XU Yuan1*
    2020, 41(1):  17-20,28.  doi:10.3969/j.issn.1007-3205.2020.01.004
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    ［Abstract］ Objective〖HTSS〗To investigate differences in the application value of CT angiography(CTA) and cervical vascular color Doppler ultrasound in diagnosis of extracranial lesions of carotid artery in patients with acute cerebral infarction(ACI).
    Methods〖HTSS〗A retrospective analysis was performed on examination data from 100 ACI patients. All patients underwent CTA, cervical vascular color Doppler ultrasound and digital subtraction angiography(DSA). The detection conditions for extracranial vascular stenosis degree of carotid artery and plaquesby CTA and cervical vascular color Doppler ultrasound were compared. Taking DSA results as golden standard,diagnostic value of the two methods for extracranial vascular stenosis degree of carotid artery was judged.
    Results〖HTSS〗The detection conditions for extracranial vascular stenosis degree of carotid artery(mild, moderate, severe, and embolization) by CTA was much better than those by cervical vascular color Doppler ultrasound. And there were significant differences only in mild and moderate stenosis(P＜0.05). The detection rate of CTA for different types plaques in internal carotid artery was better than that of cervical vascular color Doppler ultrasound(P＜0.05). There was no significant difference in detection of different types plaques between common carotid artery and external carotid artery(P＞0.05). Taking DSA results as golden standard, sensitivity, specificity, accuracy, positive predictive value and negative predictive value of CTA for diagnosis of extracranial vascular stenosis degree of carotid artery were higher than those of cervical vascular color Doppler ultrasound.There were only significant differences in sensitivity, accuracy and negative predictive value between two methods(P＜0.05).
    Conclusion〖HTSS〗The diagnostic efficiency of CTA is superior to that of cervical vascular color Doppler ultrasound in diagnosis of extracranial lesions of carotid artery in ACI patients.Especially,the advantages are more obvious in terms of the diagnosis of vascular stenosis degree and plaque type of internal carotid artery.
    Value of intrathecal isoniazid and dexamethasone combined with equal amount of normal saline replacement in the treatment of tuberculous meningitis#br#
    BAI Hong-zhong1, HAN Wei-xin2, ZHANG Xue-ping3, LI Jing2, LI Wen4*
    2020, 41(1):  21-23,33.  doi:10.3969/j.issn.1007-3205.2020.01.005
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    ［Abstract］Objective〖HTSS〗To analyze the value of intrathecal injection of isoniazid plus dexamethasone combined with saline replacement in the treatment of tuberculous meningitis.
    Methods〖HTSS〗A total of 78 patients with tuberculous meningitis were selected. They were randomly divided into reference group(intrathecal injection of isoniazid plus dexamethasone) and observation group(control group combined with equal amount of normal saline replacement treatment). The therapeutic effects of the two groups were compared.
    Results〖HTSS〗After treatment, content of protein, glucose and cell count in cerebrospinal fluid(CSF) of the two groups were lower than that before treatment, and content of chloride in CSF of the observation group was lower than that before treatment(P＜0.05). After treatment, contents of protein, glucose, cell count and chloride in CSF of the observation group were all lower than that of control group(P＜0.05). The total effective rate of observation group was higher than that of control group(P＜0.05). The adverse drug reaction rate of observation group was lower than that of control group(P＜0.05). The recovery time of headache and neck strength, CSF pressure, fever, vomiting, coma, CSF protein, CSF cell and hospitalization in the observation group were significantly shorter than those in control group(P＜0.05).
    Conclusion〖HTSS〗The treatment of tuberculous meningitis by intrathecal injection of isoniazid plus dexamethasone and replacement with equal amount of normal saline has clear clinical effect, obvious improvement of clinical indicators and symptoms, ensuring the safety of treatment, shortening the length of stay and providing guarantee for early rehabilitation.
    Evaluation of the application of No-touch obtain the great saphenous vein bridge in CABG and observation of the patency rate in the near and mid-time#br#
    AN Jing-hui， LIU Su， CHEN Zi-ying， MA Qian-li， YAN Fang， SHI Feng-wu*
    2020, 41(1):  24-28.  doi:10.3969/j.issn.1007-3205.2020.01.006
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    ［Abstract］Objective〖HTSS〗To evaluate the patency rate and the safety of No-touch great saphenous vein bridge in coronary artery bypass graft.
    Methods〖HTSS〗One hundred and five CABG cases were divided into two groups randomly. A total of 65 cases were treated with No-touch technique to obtain the great saphenous vein. 40 cases were bridged with conventional incision. The results of operation and the rate of bridging vessel patency were compared between the two groups.
    Results〖HTSS〗There was no death in the two groups. There was no significant difference between two groups(P＞0.05). There was no significant difference between two groups(P＞0.05). The incidence of numbness and pain in both legs of No-touch group was higher than that of conventional operation group(P＜0.05). There was no significant difference in the incidence of hematoma, edema and poor wound healing between two groups(P＞0.05). The patency rate of No-touch group was higher than that of routine operation group(P＜0.05).
    Conclusion〖HTSS〗The application of no touch in CABG can reduce the damage to the vein bridge and prolong the patency rate of the bridge. The No-touch method has the same safety as the conventional incision method.
    Clinical analysis of delayed cure after microvascular decompression for hemifacial spasm#br#
    SHI Hao-wei, LI Yang, QIAN Tao, SHI Hai-liang*
    2020, 41(1):  29-33.  doi:10.3969/j.issn.1007-3205.2020.01.007
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    ［Abstract］ Objective〖HTSS〗To explore the related factors of delayed cure after microvacular decompression(MVD) in patients with hemifacial spasm(HFS).
    Methods〖HTSS〗Clinical data of 220 HFS patients were conducted the retrospective analysis. All patients were treated with MVD. Relevant influencing factors of delaying cure for patients after operation were analyzed.
    Results〖HTSS〗After operation, 176 patients were cured immediately, while 44 patients delayed cure. After one-year follow-up visiting, healing time of 44 patients in the delayed cure group was 10 d-12 month.The analysis of the influencing factors of delayed cure after operation showed that there was no significant difference between delayed cure and gender, age and the size of the responsible artery(P＞0.05). There was no significant difference in the history, severity of symptoms and monitoring of abnormal facial muscles between two groups(P＜0.05). Logistic regression analysis showed that medical history and severity of symptoms were the influencing factors of delayed cure.
    Conclusion  The medical history and severity of symptoms are the influencing factors of delayed cure. The longer medical history is, the easier delayed cure will have. The severer spasm symptoms are, the easier delayed cure will have. Delayed cure after MVD in HFS patients is more common. Analysis of the influencing factors of delayed cure after MVD can improve the clinical symptoms and prognosis of patients.
    Evaluation of the effect of anti-osteoporosis treatment by quantitative CT#br#
    WANG Yi-feng1, DONG Wei1, TANG Li-jian2, YANG Hui3, LI Pei-xiu4, FAN Guo-feng1*
    2020, 41(1):  34-36,41.  doi:10.3969/j.issn.1007-3205.2020.01.008
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    ［Abstract］Objective〖HTSS〗To explore the feasibility of quantitative CT in evaluating the effect of anti-osteoporosis treatment.
    Methods〖HTSS〗The patients with postmenopausal osteoporosis were treated with bisphosphonate. Serum β-C-terminal collagen of type Ⅰ collagen(β-CTX), dual energy X-ray bone density and quantitative CT bone density were measured before and 6 months after treatment. According to the change of serum β-CTX, the patients were divided into two groups: the effective group and the ineffective group.
    Results〖HTSS〗A total of 220 patients were included. 211 patients whose serum β-CTX content decreased by more than 50% were regarded as the treatment effective group， 9 patients whose serum β-CTX content decreased less than 50% were regarded as the treatment ineffective group. After 6 months of oral bisphosphonates treatment, there was no significant change in dualeneopausal X-ray absorptiomtry bone mineral density(BMD) in the effective group and the ineffective group. Quantitative CT BMD in the effective group indicated that BMD increased, but there was no significant change in the ineffective group.
    Conclusion〖HTSS〗The detection of serum β-CTX and quantitative CT BMD can evaluate the effect of anti bone resorption in postmenopausal osteoporosis patients, and the two detection methods are complementary to each other, providing guidance for the treatment of postmenopausal osteoporosis.
    In vitro biomechanical experiment and clinical application of hollow titanium rod in the treatment of early necrosis of femoral head#br#
    HU Chang-bo,YANG Xin-ming*
    2020, 41(1):  37-41.  doi:10.3969/j.issn.1007-3205.2020.01.009
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    ［Abstract］ Objective〖HTSS〗To assessthe titanium rob for the treatment of femoral head osteonecrosis and evaluate its biomechanical efficacy.
    Methods〖HTSS〗Fifteen normal adult femoral specimens were randomly divided into three groups: group A,normal control； group B, the femoral heads were dealt with decompression; group C, the femoral heads were dealt with the titanium rob. Femoral heads were subjected to axial torque. Strain of fossa trochanterica, femoral calcar and subtrochantarer was calculated and the maximum load of femoral sink and damage was measured.
    Results〖HTSS〗No significant difference of the strain of fossa trochanterica and femoral calcar was noted between group A and group C under 300 N loading condition. Compared to group A or group C, group B had lower strain of fossa trochanterica and femoral calcar under 300 N loading condition(P＜0.05). No significant difference of the strain of subtrochantarer was noted between group A and group C under 300 N loading condition. Compared to group A or group C, group B had higher strain of subtrochantarer under 300 N loading condition(P＜0.05).  No significant difference of the maximum load of femoral collapse was noted between group A and group C. Compared to group A or group C, group B had lower maximum load of femoral collapse(P＜0.05).
    Conclusion〖HTSS〗This new type of the titanium rob graft in femoral head can effectively maintain the biomechanical performance of femoral head and neck, which is worthy of being clinical applied.
    Observation on the short-term curative effect of expanded ACDF in the treatment of cervical spondylotic myelopathy with the aid of microscope#br#
    ZHANG Fei, ZHANG Wei*, LI Ze-yang, SUN Ya-peng, LI Jia-qi, GUO Lei
    2020, 41(1):  42-45.  doi:10.3969/j.issn.1007-3205.2020.01.0010
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    ［Abstract］Objective〖HTSS〗To analyze the short-term curative effect of expanded anterior cervical discectomy and fusion（ACDF） in the treatment of cervical spondylotic myelopathy.
    Methods〖HTSS〗Clinical data of 138 cervical spondylotic myelopathy patients were retrospectively analyzed. All the patients were divided into expanding ACDF group(68 cases) and the traditional ACDF group(70 cases) according to different therapeutic methods. The duration of surgery, intraoperative blood loss, incidence of complications, Japanese Orthopaedic Association（JOA） score, intervertebral distraction index（IDI） and fusion rates were compared between two groups.
    Results〖HTSS〗Operation time of group B was significantly shorter than group A(P＜0.05), and complication rate of group B was significantly shorter than group A(P＜0.05), but bleeding amount of two groups showed no significant difference(P＞0.05). The JOA score of expand ACDF was higher than that of traditional ACDF group（P＜0.05）. The IDI of the two group showed a reduce compare to immediately postoperative. IDI of group A were significantly greater than group B, both showed immediately postoperative and 3 months after（P＜0.05）. The fusion rate(P＜0.05) of group B was significantly superior to group A3 after 3 months.
    Conclusion〖HTSS〗Endoscope-assisted expand ACDF surgery is a safe and effective surgery for the treatment of cervical spondylosis. This operation can enlarge the operative indications of anterior portion, improve the surgical curative effect, and can reduce the incidence of the axial symptoms.
    Analysis on risk factors of post-operative lateral femoral cutaneous nerve palsy after shoulder surgery in the beach chair position#br#
    FENG Gui-yin, LI Ling, FAN Yan, CUI Yong-chun, QI Yun-lin, LI Chen-chen
    2020, 41(1):  46-49.  doi:10.3969/j.issn.1007-3205.2020.01.011
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    ［Abstract］Objective〖HTSS〗To explore the risk factors of post-operative lateral femoral cutaneous nerve palsy(LFCNP) after shoulder surgery in the beach chair position.
    Methods〖HTSS〗A retrospective analysis was conducted according to the medical records of 254 patients undergoing shoulder surgery in the beach chair. These patients were divided into LFCNP group(8 patients) and non-LFCNP group(246 patients) based on post-operative examination. The demographic data, American Society of Anesthesiologists(ASA) physical status, pre-operative complication(hypertension, diabetes mellitus), anesthesia type, peri-operative transfusion, urine volume, blood loss, and surgical duration were collected. Univariate analysis and bivariate logistic regression were performed to account for association between post-operative LFCNP and independent risk factors.
    Results〖HTSS〗Univariate analysis showed that the independent variables of LFCNP were body mass index(BMI) and surgical duration(P＜0.05), but there was no significant difference of age, gender, height, weight, ASA physical status, pre-operative complication(hypertension, diabetes mellitus), anesthesia type, peri-operative transfusion, urine volume, blood loss between these two groups. In a bivariate Logistic regression model, BMI>28 and surgical duration >2 h were correlated with post-operative LFCNP.
    Conclusion〖HTSS〗Our retrospective study suggested that BMI>28 and surgical duration >2 h were independent risk factors of post-operative LFCNP after shoulder surgery in the beach chair position.
    Study on the effect of circulating pressure and cold therapy on soft tissue swelling in patients with ankle fracture
    SHI Sha1, DUAN Hui-xia1, LI Xiu-ting1, QI Su-fen2, ZHAO Shu-e3*
    2020, 41(1):  50-53.  doi:10.3969/j.issn.1007-3205.2020.01.012
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    ［Abstract］Objective〖HTSS〗To observe the effect of cold and circulatory pressure therapy on soft tissue swelling of ankle fracture.
    Methods〖HTSS〗Sixty-two patients with ankle fracture were randomly divided into the experimental group and the control group, each with 31 patients. After admission to the experimental group, the patients in the experimental group were treated with detumescence by circulating pressure cold therapy device. Patients in the control group were given routine orthopedic care and treated with ice packs. Visual Analogue Scale(VAS) score was used for pain assessment before,24,48,and 72 hours after treatment. The swelling of the affected limb was evaluated by measuring the swelling value of fixed point position(the circumference of medial and lateral malleolus).The incidence of tension blisters and preoperative waiting time were compared between the two groups.
    Results〖HTSS〗With the extension of time, the swelling value and VAS score of affected limb in both groups showed a decreasing trend, and the reduction range of the swelling value and VAS score of affected limb in the experimental group was greater than that in the control group, and the interaction difference between groups, time points and between groups was statistically significant(P＜0.05).The incidence of tension blisters in the experimental group was significantly lower than that in the control group, and the preoperative waiting time was shorter than that in the control group, with statistically significant difference(P＜0.05).
    Conclusion〖HTSS〗Early application of circulatory compression and cold therapy in patients with ankle fractures is beneficial to reduce the pain and soft tissue swelling and reduce the incidence of tension blisters.
    Effect of androgen on telomeres length in aplastic anemia patients#br#
    ZHANG Hui-min, YUAN Li-qian, SHI Kui-zhu, GAO Xi-hua, XIE Qing-qing, TAO Chao-xin
    2020, 41(1):  54-57.  doi:10.3969/j.issn.1007-3205.2020.01.013
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    ［Abstract］Objective〖HTSS〗To explore the effect of androgen on telomeres in aplastic anemia（AA） patients, and the effect of telomeres in the pathogenesis of AA.
    Methods〖HTSS〗The patients were collected according to the AA clinical diagnostic criteria. They were divided into two groups, cyclosporin A(CsA)+androgen and CsA respectively, 15 cases in each group. The genomic DNA of peripheral blood was extracted by nucleic acid extractor and nucleic acid extraction kit. The relative length of telomere in peripheral blood leukocytes was measured by real-time fluorescence quantitative PCR and compared with that of 15 healthy controls.
    Results〖HTSS〗The relative T/S ratio of the telomere length in AA patients was significantly shorter than that in the normal control group(P＜0.05). After treatment, the relative T/S ratio of the telomere length in the CsA group was reduced volatility,and that of the CsA+androgen group was increased. The relative T/S ratio of the telomere length in the CsA+androgen group was evidently higher than that in the CsA group.The differences between groups,time points, interaction of groups and time points were statistically significant(P＜0.05). The hematological improvement of CsA+androgen was better than that of CsA group at 12 months after treatment(P＜0.05).
    Conclusion〖HTSS〗Androgen can regulate telomere length in AA patients, and the length of telomeres increased after androgen therapy. Androgen is effective in the treatment of AA with telomere dysfunction, and clinical hematologic improvement can be achieved.
    Effect of low frequency electrical stimulation on humeral fracture with radial nerve injury
    LIU Min1, LI Song2, WANG Hong-lian1, LI Yu-min1， ZHAO Yun1， SHI Han-wen3
    2020, 41(1):  58-60,76.  doi:10.3969/j.issn.1007-3205.2020.01.014
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    ［Abstract］ Objective〖HTSS〗To observe the effectiveness of low frequency electrical stimulation on the treatment of humeral shaft fracture complicated with radial nerve injury and its effect on quality of life.
    Methods〖HTSS〗A total of 80 patients with radial nerve injury after humeral fracture were randomly divided into treatment group and control group. The control group was treated with conventional drugs, and the treatment group was treated with low frequency electric stimulation on the basis of the control group. Before and after treatment, the muscle strength of the extensor carpi and extensor digitorum was assessed, and the electromyogram was measured, and the MOS item short from health survey(SF-36) was evaluated.
    Results〖HTSS〗After treatment, the number of extensor carpal muscle grade 4-5 in the treatment group was significantly higher than that before treatment, and the number of total extensor muscle grade 4-5 in the two groups was significantly higher than that before treatment(P＜0.05). After treatment, the muscle strength of extensor carpal muscle and total extensor finger muscle in the treatment group was much better than that in the control group(P＜0.05). After treatment, the amplitude of MCV and wave in the two groups were significantly higher than those before treatment(P＜0.05). After treatment, the amplitude of MCV and wave in the treatment group were higher than those in the control group(P＜0.05). After treatment, the scores of social functioning, physical functioning, role  emotional, mental  health and general health in the treatment group were higher than those in the control group(P＜0.05).
    Conclusion〖HTSS〗Low frequency pulse electrotherapy can effectively improve the muscle strength and motor function of upper limbs in patients with radial nerve injury, and can improve the quality of life of patients.
    Assessment of the hemodynamic changes of carotid and cerebral arteries in hypertrophic cardiomyopathy with color Doppler ultrasound #br#
    ZHAO Yong-feng, WANG Jing, ZUO Lei, KANG Nan, YANG Fan, LIU Li-wen*
    2020, 41(1):  61-65,71.  doi:10.3969/j.issn.1007-3205.2020.01.015
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    ［Abstract］Objective〖HTSS〗To investigate the hemodynamic changes of carotid and cerebral arteries in hypertrophic cardiomyopathy(HCM).
    Methods〖HTSS〗Totally 40 HCM patients and 25 normal controls were enrolled. All of them received echocardiography, carotid and cerebral arteries color Doppler ultrasound. Echocardiographic parameters included maximal left ventricular wall thickness(MLVWT), left ventricular end-diastolic diameter(LVEDD), left ventricular end-diastolic and systolic volume(LVEDV and LVESV), left ventricular ejection fraction(LVEF), left ventricular outflow tract pressure gradients(LVOT-PG), left atrial diameter(LAD), and mitral valve inflow and annular early diastolic velocity(E and e′). Besides, the carotid and cerebral arteries were involved including bilateral common carotid artery(CCA), internal carotid artery(ICA), middle cerebral artery(MCA), vertebral artery(VA), anterior cerebral artery(ACA), posterior cerebral artery(PCA), vertebral artery-intracranial(VA-IC) and basilar artery(BA). The ultrasound parameters of these arteries, such as peak systolic velocity(PSV), maximum end-diastolic velocity(EDV), average end-diastolic velocity(MDV), pulsative index(PI), resistance index(RI), velocity-time integrals(VTI), S/D, mean velocity(Mean) were all measured and compared.
    Results〖HTSS〗The ratios of MLVWT, LVOT-PG, LAD, and E/e′ in the HCM group were significantly higher than those in the control group; EDV, MDV, and VTI of bilateral CCA were lower than those in the control group, and PI and RI were higher. PSV, EDV and MDV in right ICA were lower than those in the control group, PI and RI were higher.  EDV in left ICA was lower than that in the control group, PI and RI were higher. PI and RI of the right VA were higher than the control group. EDV in left VA was lower than that in normal control group, PI and RI were higher. RI and Mean in left MCA were higher than that of the control group. VTI of bilateral PCA and left ACA  were higher than that of the control group; PSV, PI, RI and SD of right va-ic were higher than that of the control group, the differences were statistically significant(P＜0.05).
    Conclusion〖HTSS〗In addition to cardiac dysfunction, abnormal hemodynamic changes of carotid and cerebral arteries may also occurr in HCM. Therefore, carotid and cerebral vascular ultrasound should be performed routinely in HCM, which is of great significance for comprehensive clinical evaluation and early intervention.
    Diagnostic value of S-Detect combined with elastography in benign and malignant evaluation of breast BI-RADS4 nodules
    JIN Xiu-li1, LI Feng-juan1, WAN Hui2, DU Jian-wen1*, WANG Hong1
    2020, 41(1):  66-71.  doi:10.3969/j.issn.1007-3205.2020.01.016
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    ［Abstract］ Objective〖HTSS〗To explore the value of combined application of S-Detect and elastography in the diagnosis of benign and malignant breast Breast Imaging Reporting and Data System 4（BI-RADS4） nodules.
    Methods〖HTSS〗Two-dimensional ultrasound, elastic imaging and S-Detect were used to distinguish the benign and malignant lesions of 120 breast nodules in BI-RADS4 class in 110 female patients. The results of postoperative pathological diagnosis were used as the gold standard. The diagnostic efficiency of two-dimensional ultrasound alone combined with elastic imaging and the combination of S-Detect and elastic imaging were compared and analyzed.
    Results〖HTSS〗The sensitivity, specificity and accuracy of two-dimensional ultrasound combined with elastography were 73.68%, 82.92% and 80.00%, respectively. At the same time, the sensitivity, specificity and accuracy of S-Detect and elastography were 86.84%, 87.80% and 86.66%, respectively. The area under of ROC curve of two-dimensional ultrasound combined with elastic imaging and S-Detect combined with elastic imaging in the diagnosis of benign and malignant breast BI-RADS4 nodules were 0.746 and 0.942, respectively. The diagnostic efficiency of two-dimensional ultrasound combined with S-Detect and elastic imaging was better than that of elastic imaging alone(P＜0.05).
    Conclusion〖HTSS〗S-Detect combined with elastography can be used as a new supplementary diagnostic method of two-dimensional ultrasound in the diagnosis of breast nodules. The assistant two-dimensional ultrasound diagnosis can significantly improve the effectiveness of differential diagnosis of benign and malignant breast nodules in BI-RADS4.
    The application and quality control of 1〖DK〗∶1 DR chest adiography in whole body radiotherapy
    ZHANG Xin-qi， LYU Meng-ting, DU Kun-long, SUN Ze-yu, BAO Yong-chao, ZHANG Yin-feng
    2020, 41(1):  72-76.  doi:10.3969/j.issn.1007-3205.2020.01.017
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    ［Abstract］Objective〖HTSS〗To investigate the accuracy and quality control of 1〖DK〗∶1 DR chest radiography in whole-body radiotherapy.
    Methods〖HTSS〗The 47 patients who met the inclusion criteria were selected for 1〖DK〗∶1 DR chest radiography. According to the chest body thickness(front and back diameter), the patients were divided into A, B and C 3 groups, 17 in group A(body thickness H1≤ 20 cm), 15 in group B(body thickness H2 >20-24 cm), and 15 in group C(body thickness H3 >24 cm). According to the shape and size of lung field in 1〖DK〗∶1 DR chest radiograph, a lung block was made to shield the lung effectively in whole body radiotherapy. Set the left and right diameter of the patient′s chest as S1, and the distance between two chest walls of the chest image as S2. The printing proportion of photos before quality control was 100%, after quality control, the printing proportion of photos set according to the chest thickness range of patients was 93%, 91% and 89%, respectively. S1 and S2 of patients before and after quality control were compared. At the same time, the changes of S2 in the same patient under different respiratory states were compared.
    Results〖HTSS〗Before quality control, the distance between two sides of chest wall(S2) in group A, B and C3 was significantly greater than that in group S1(P＜0.05). After quality control, there was no significant difference between left and right chest diameter(S1) and the distance between two sides of chest wall(S2) in group A, B and C(P＞0.05). The deep breath holding state S2 of the same patient was significantly higher than that of the calm breath holding state S2(P＜0.05).
    Conclusion〖HTSS〗After control, according to the thickness of the patient′s chest, the printing proportion of the chest image was determined to be 93%, 91% and 89%, respectively. The DR chest film with the same lung field and lung size on the DR chest film, 1〖DK〗∶1 DR chest film, can be obtained. The lung lead block made according to it can shield the lung accurately in the whole body radiotherapy, which is worth popularizing and applying.
    Application of copper additional filtration with different thicknesses in skeleton digital radiograph
    WANG Shi-jie1, WU Hui-zhao1*, ZHANG Xin-qi2, CHEN Xin-yi1, DU Kun-long1, SUN Ze-yu1
    2020, 41(1):  77-80.  doi:10.3969/j.issn.1007-3205.2020.01.018
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    ［Abstract］Objective〖HTSS〗To investigate the application value of copper additional filtration with different thickness in bone digital radiography（DR）.
    Methods〖HTSS〗The equivalent phantom of the human body was used for bone DR. The thick parts such as head, spine and pelvis were examined as automatic exposure control（AEC） group by AEC system using the ionization chamber.The thin parts such as limbs and joints without AEC were examined as non-AEC group. The copper additional filters with different thickness of 0.1 mm, 0.2 mm and 0.3 mm were selected for exposure. The surface incident dose, imaging quality score and tube current of different thickness copper additional filter groups were compared.
    Results〖HTSS〗There were significant differences in the surface incident dose of four different thickness copper additional filters in non-AEC group. By pairwise comparison, the surface incident dose without additional filtration was greater than 0.1 mm, 0.2 mm and 0.3 mm copper additional filtration respectively, while the surface incident dose of 0.1 mm copper additional filtration was greater than 0.3 mm copper additional filtration, with statistically significant difference(P＜0.05). In non-AEC group, there were no significant differences in imaging quality scores and tube current of four different thickness copper additional filters(P＞0.05). There were significant differences in the surface incident dose and the tube current of four different thickness copper additional filters in AEC group. By pairwise comparison, the surface incident dose without additional filtration was greater than 0.3 mm copper additional filtration, while the tube current was less than 0.3 mm copper additional filtration, the difference was statistically significant(P＜0.05). In AEC group, there were no significant differences in imaging quality scores of four different thickness copper additional filters(P＞0.05).
    Conclusion〖HTSS〗The copper additional filtration can reduce the examination dose of skeletal system DR effectively on the premise of ensuring the diagnostic quality, and achieve low-dose radiography of skeletal system.
    Application of laryngeal mask in airway management of patients undergoing hysteroscopic surgery#br#
    YANG Zhi-hua
    2020, 41(1):  81-84,98.  doi:10.3969/j.issn.1007-3205.2020.01.019
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    ［Abstract］Objective〖HTSS〗To explore the application effects of laryngeal mask in airway management of patients undergoing hysteroscopic surgery.
    Methods〖HTSS〗A total of 150 patients who underwent gynecological hysteroscopic surgery under general anesthesia were selected and randomly divided into three groups, with 50 cases in each group. T group was given tracheal intubation to establish a safe airway, and P group was given ProSeal laryngeal mask, and S group was given Supreme laryngeal mask. The frequencies and success rate of tracheal intubation or laryngeal mask placement, intraoperative conditions, vital signs and complications were observed among three groups.
    Results〖HTSS〗The success rate of one-time placement in P group and S group was higher than that in T group(P＜0.05). The placement time in S group was smaller than that in T group, and the extubation time in P group and S group was smaller than that in T group, and the airway sealing pressure was higher than that in T group(P＜0.05). There were no significant differences in the success rate, placement time, extubation time and airway sealing pressure of laryngeal mask placement between P group and S group(P＞0.05). There were no significant differences in anesthesia time and recovery time among three groups(P＞0.05). The mean artery pressure(MAP), heart rate(HR) and pulse oxygen saturation(SpO2) in the three groups were fluctuating at each time point, and there were statistically significant differences in the time-point effects and interactions of time-point and between-groups(P＜0.05), and there were no significant differences in the MAP, HR and SpO2 among the three groups at each time point(P＞0.05). The incidence rates of postoperative sore throat and dysphagia in P group and S group were lower than those in T group(P＜0.05). There were no significant differences in the incidence rates of bucking, laryngospasm, gaseous distention and jaw pain among the three groups(P＞0.05).
    Conclusion〖HTSS〗Compared with tracheal intubation, hysteroscopic surgery under general anesthesia for laryngeal mask to establish a safe airway is easy to operate and easy to place, and it has better ventilation effects and airway sealing, and has no effects on hemodynamics during surgery and fewer complications after extubation. ProSeal laryngeal mask and Supreme laryngeal mask are well-applied for safe airway management in patients undergoing hysteroscopic surgery.
    Effect of femoral nerve combined with sciatic nerve block and patient-controlled intravenous analgesia on postoperative analgesia after total knee arthroplasty#br#
    LIU Yuan-hui, QIU Liu-ji, LIN Xiao-feng, LUO De-xing
    2020, 41(1):  85-88,93.  doi:10.3969/j.issn.1007-3205.2020.01.020
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    ［Abstract］ Objective〖HTSS〗To observe the effect of ultrasound-guided femoral nerve combined with sciatic nerve block and patient-controlled intravenous analgesia for postoperative analgesia after total knee arthroplasty.
    Methods〖HTSS〗One hundred patients undergoing elective fitting line of knee replacement as object, according to the random indicator method were divided into two groups, 50 cases in each group. At the end of operation, the observation group was treated with femoral nerve combined with sciatic nerve block analgesia under the guidance of ultrasound, and the control group was treated with controlled intravenous analgesia. Visual Analogue Scale(VAS) was used to compare the analgesic effects of the two groups, and the postoperative recovery and adverse reactions were observed.
    Results〖HTSS〗The VAS score of the observation group was not statistically significant compared with the control group(P＞0.05), and the VAS score of 4, 12, 24, 48, 72 h after the operation was significantly lower than that of the control group(P＜0.05). The time of active straight leg elevation of 45 degrees and active flexion of knee up to 90 degrees were significantly shorter than that of the control group(P＜0.05), and the knee joint activity of the discharged knee was significantly higher than that of the control group(P＜0.05). After 72 h, the incidence of adverse reactions in the observation group was 24.00%, and the incidence of adverse reactions in the control group was 46.00%, the difference was statistically significant(P＜0.05).
    Conclusion〖HTSS〗Ultrasound guided femoral nerve combined with sciatic nerve block can effectively relieve the pain after knee replacement, reduce the incidence of adverse reactions and accelerate functional recovery.
    Apoptotic cells regulate the level of IL-12 family in macrophages#br#
    LIU Bing-hui1， GONG Zhi-ping1， MEI Yan-fang1， YANG Tao1， FENG Jun1, QIAN Xue-song2*
    2020, 41(1):  89-93.  doi:10.3969/j.issn.1007-3205.2020.01.021
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    ［Abstract］Objective〖HTSS〗To investigate the effect of apoptotic cells on expression of interleukin-12（IL-12） family cytokines in macrophages.
    Methods〖HTSS〗RAW264.7 (RAW cells) from the mouse macrophage cell lineage and mouse peritoneal macrophages were divided into 4 groups respectively: blank control group, lipopolysaccharide(LPS) stimulation group, apoptotic(apo) cell stimulation group, LPS+apo cell stimulation group, the levels of mIL-12p35, mIL-12p40 and mIL-23p19 in macrophage cell of each group before and after the stimulation were detected by real-time quantitative polymease chain reaction（PCR）. The contents of IL-10, prostaglandin E2(PGE2) and transforming growth factor-β (TGF-β) in RAW cell supernatant were detected by enzyme linked immunosorbent assay(ELISA).
    Results〖HTSS〗The mRNA expression level of IL-12p35, IL-12p40 and IL-23p19 in RAW cells or mouse peritoneal macrophages were higher in LPS group than in blank control group and apo group. The mRNA level of IL-12p35, IL-12p40 and IL-23p19 in the LPS+apo group were higher than those in the blank control group and in the apo group, and lower than those in the LPS group(P＜0.05). The concentrations of IL-10, PGE2 and TGF-β in the LPS group or LPS+apo group were all higher than those in the control group, and  the concentrations of TGF-β in LPS+apo group were significantly higher than those in the LPS group(P＜0.05), while the differences of IL-10 or PGE2 concentrations in the LPS group and LPS+apo group were not statistically significant(P＞0.05).
    Conclusion〖HTSS〗Apoptotic cells inhibit the expression of IL-12p35, IL-12p40 and IL-23p19 mRNA in activated macrophages. Apoptotic cells have no effect on the secretion of IL-10 and PGE2, but can promote the secretion of TGF-β in macrophages.
    Comparison of the two methods in the detection of goat-milk containing anti-Helicobacter pylori specific antibody and preliminary clinical effect#br#
    YAN Xi1， FAN Jian-ping2， WANG Xian3， FENG Jian-fei2， ZHAO Lian-mei4， SHAN Bao-en4*
    2020, 41(1):  94-98.  doi:10.3969/j.issn.1007-3205.2020.01.022
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    ［Abstract］ Objective〖HTSS〗To compare two detection methods of goat-milk containing anti-Helicobacter pylori specific antibody, and to observe the clinical effect of antibody goat-milk.
    Methods〖HTSS〗Goat-milk containing antibodies against Helicobacter pylori was prepared by immunizing dairy goat with international standard strains (ATCC26695, ATCC26695 and SS1 mixture). The valence of antibody against Helicobacter pylori in goat-milk was detected by double immunodiffusion test and enzyme linked immunosorbent assay(ELISA), and then compare the two methods. 30 subjects were randomly assigned to two groups. The test group were treated with goat-milk containing Helicobacter pylori specific antibody, and control group with common goat-milk, drink for 30 days continuously. The effect was assessed by the 14C urea breath test(14C-UBT).
    Results〖HTSS〗The results of the two methods all confirmed the existence of anti-Helicobacter pylori specific antibodies in goat milk, ELISA test show that the content of antibodies in the two goat-milk was higher than that in the control, the difference was statistically significant(P＜0.05), and with the increase of dilution ratio, the antibody content decreased gradually. There was no significant difference in antibody content between the two kinds of goat milk(P＞0.05). Preliminary clinical trials have shown that the effective rate of the experimental group was 66.6% after taking the antibody goat-milk, and the difference was statistically significant(P＜0.05) compared with the control group.
    Conclusion〖HTSS〗Both of the two detection methods can better prove the existence of anti-Helicobacter pylori specific antibodies in goat-milk. Methed of ELISA is simple and fast, which can more accurate quantitatively detect antibody content. And the results are more reliable. Clinical trials have shown that goat-milk containing Helicobacter pylori specific antibody can effectively reduce the value of 14C-UBT in patients, it has a good prospect of clinical application.