Loading...

Table of Content

    25 July 2019, Volume 40 Issue 7
    Previous Issue    Next Issue
    The preliminary study on correlation between transmembrane protein ESDN and vascular hyperplasia
    GONG Yue, WANG Qisong, WANG Fanke, MENG Zeqi, LIU Xiaoning, NIE Lei*
    2019, 40(7):  753-757,798.  doi:10.3969/j.issn.10073205.2019.07.003
    Asbtract ( 646 )   PDF (1604KB) ( 124 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To investigate the correlation of transmembrane protein endothelial and smooth muscle cellderived neuropilinlike molecule(ESDN) expression and vascular hyperplasia.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The intimal hyperplasia model of the Esdn knockout mice and control wild type mice(WT) were prepared by the common carotid artery ligation. HE staining was used to detect the neointima fromation at different time points. Western blot was used to detect the expression of smooth muscle αactin(SM αactin), SM22α and osteopontin(OPN), the phenotype marker genes in vascular smooth muscle cells. Immunofluorescence was used to detect the SM αactin protein expression and distribution in aorta.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓At 7, 14 and 21 days after arterial ligation, the area of the intimal hyperplasia and the I/M ratio were significantly increased in the Esdn-/- arteries compared with WT control arteries. The expression of SM αactin and SM22α were decreased. However, the expression of OPN, the synthetic marker gene, was increased in the Esdn-/- arteries at 3, 7, 14 and 21 days after arterial ligation. Furthermore, the expression of SM αactin was enhanced in the media area of aorta in Esdn-/- arteries using immunofluorescence staining.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The expression of ESDN may be involved in vascular smooth muscle cell phenotypic switching and vascular remodeling.
    Effect of curcumin on the expression of integrin β3 and LIF in endometrium of diabetic rats#br#
    ZHAO Chunfang1， SHEN Yaxin2， GONG Miao1， ZHANG Lei1， ZHANG Yanan1*
    2019, 40(7):  758-762.  doi:10.3969/j.issn.10073205.2019.07.004
    Asbtract ( 702 )   PDF (2156KB) ( 159 )  
    Related Articles | Metrics
    ［Abstract］ Objective〖HTSS〗〓To investigate the effect of curcumin on the expression of integrin β3 and leukemia inhibitory factor(LIF) in endometrium of diabetic rats.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirty female SpragueDawley rats were divided into  control group,Diabetes model group and curcumin compound group randomly. The normal 〖JP2〗controls and diabetes model group served with edible corn oil 2.5 mL/kg for 10 weeks, while the curcumin compound group were treated with 250 mg·〖JP〗kg-1·d-1 curcumin compound suspension for the same time. After 10 weeks, the ratio of male to female was 3〖DK〗∶2 for mating. The vagina of the female was checked next day. The fetus was found to be the first day of pregnancy and were sacrificed in the fourth day. Blood glucose and insulin were measured separately. The morphology of endometrial epithelium were observed by HE staining and immunohistochemistry. Western blot analysis were used to analyze the changing of the endometrial integrin β3 and LIF after treatment with curcumin for 10 weeks.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the normal group, the blood glucose and insulin were significantly increased in the diabetic group(P＜005), and the expression of integrin β3 and LIF in the endometrium were significantly decreased(P＜005). Compared with the diabetes group, the blood glucose and insulin were significantly lower(P＜005), and the expression of integrin β3 and LIF in the endometrium were significantly increased(P＜005) in the curcumin compound treatment group.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Curcumin can lower blood sugar and insulin, increase the expression of endometrial integrin β3 and LIF protein, and finally improve endometrial receptivity.
    The clinical and coronary angiography characteristics in patients with premature myocardial infarction
    ZHANG Lili1， LIU Chuanfen1， CHEN Hong1*， DING Chunhua2， WU Manyan1， CUI Yuxia1
    2019, 40(7):  763-767,802.  doi:10.3969/j.issn.10073205.2019.07.005
    Asbtract ( 663 )   PDF (411KB) ( 126 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To explore the clinical and coronary angiography features in patients with premature myocardial infarction(pMI)．
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred and fiftynine patients with premature STsegment elevation myocardial infarction(pMI group) and 190 patients with nonpremature STsegment elevation myocardial infarction(nonpMI group) were collected. General data, laboratory examination results, coronary angiography results and hospital complications of the two groups were compared.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓In the pMI group, the proportion of males, BMI, smoking, family history of earlyonset coronary heart disease, hyperlipidemia, diastolic blood pressure on admission, and cardiac function grade 2 or above was higher than that in the nonpMI group. In the pMI group, the proportion of hypertension, diabetes, stroke, kidney disease and anemia was lower than that in the nonpMI group. The levels of LDLC, total cholesterol, triglyceride and nonHDLC in the pMI group were all higher than those in the nonpMI group. The hemoglobin level in the pMI group was higher than that in the nonpMI group, and the serum creatinine and blood glucose levels were lower than that in the nonpMI group. The proportion of patients in the pMI group receiving coronary angiography and emergency PCI during hospitalization was higher than that in the nonpMI group. The left anterior descending artery was the most common infarctrelated vessel in the pMI group, which was higher than that in the nonpMI group. The proportion of single coronary artery involvement was higher in the pMI group. In the lesion site, the involvement of left main artery and right coronary artery was higher in the nonpMI group. The incidence of serious complications and mortality in the pMI group were lower than those in the nonpMI group.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The proportion of male, smoking, family history of early onset coronary heart disease, BMI and blood lipid levels of the patients with pMI were all higher than the nonpMI patients. Left anterior descending arterys were the most common infarctrelated vasculars in pMI patients. The serious complications and mortality in hospital in pMI patients were lower than those in nonpMI patients.
    Correlation of serum placental growth factor and NTproBNP and recent prognosis of elderly patients with chronic heart failure #br#
    PENG Xue, WANG Wenjuan, NIU Jieting
    2019, 40(7):  768-772.  doi:10.3969/j.issn.10073205.2019.07.006
    Asbtract ( 504 )   PDF (399KB) ( 134 )  
    Related Articles | Metrics
    ［Abstract］ Objective〖HTSS〗〓To investigate the correlation of serum placental growth factor(PlGF) and Nterminal probrain natriuretic peptide type B(NTProBNP) and recent prognosis of elderly patients with chronic heart failure(CHF).
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One huandred and eightyfive elderly CHF patients in our hospital were selected as CHF group and 60 patients were selected as control group during the same period. The general clinical data and serum PlGF level were collected. The main adverse cardiovascular events(MACE) were divided into MACE group and nonmace group following 6 months of followup. The relationship between PlGF and MACE was analyzed by Logistic regression equation.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Serum PlGF CHF group, NTproBNP, left ventricular enddiastolic diameter(LVEDD), left ventricular endsystolic diameter(LVESD) were higher than the control group, left ventricular ejection fraction(LVEF) and E/A were lower than the control group, comparing differences statistically significant(P＜005). The serum PlGF level was increased gradually(P＜005) with the increasing of NYHA classification. Pearson correlation analysis showed that PlGF was positively correlated with NTproBNP presentation; serum PlGF, NYHA, NTproBNP, LVEDD in MACE group were higher than nonmace group, LVEF and E/A were lower than nonmace group, and the difference was statistically significant(P＜005). Multiple Logistic regression analysis showed that PlGF level was an independent risk factor for MACE in elderly CHF patients.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Serum PlGF expression significantly higher in elderly patients with CHF, with deterioration of cardiac function level increased significantly, and positively correlated with the NTproBNP, It may be a risk factor for poor prognosis in the near future and should be paid attention to clinically.
    Relationship between miRNA29a3p and myocardial fibrosis in patients of hypertension with left ventricular hypertrophy#br#
    HAN Wei1， HAN Yancong2*， QIU Yonghua1， HUANG Lirong1， ZHENG Xiaopeng1， LIU Jiarong1
    2019, 40(7):  773-776,793.  doi:10.3969/j.issn.10073205.2019.07.007
    Asbtract ( 617 )   PDF (387KB) ( 85 )  
    Related Articles | Metrics
    ［Abstract］ Objective〖HTSS〗〓To explore the relationship between miRNA29a3p and myocardial fibrosis in patients of hypertension with left ventricular hypertrophy.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred and sixty  patients with grade 2 or higher primary hypertension were divided into pure hypertension group(80 cases) and hypertension with left ventricular hypertrophy group(80 cases), the healthy people in our hospital physical examination center were enrolled as control group（90 cases）. The miRNA29a3p,matrix metalloproteinases9(MMP9), procollagen Ⅰ and Ⅲ in serum were detected in three groups. The relationship between miRNA29a3p and MMP9,PINP and PIIINP in hypertension with left ventricular hypertrophy group were analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Serum miRNA29a3p levels in the hypertension group and hypertension with left ventricular hypertrophy were higher than those in the control group, and the levels of miRNA29a3p in the hypertension with left ventricular hypertrophy group were higher than those in the hypertension group(P＜005). The levels of serum type Ⅰ collagen, type Ⅲ  collagen and matrix metalloproteinase9 in the hypertension group and hypertension with left ventricular hypertrophy were higher than those in the control group. The levels of serum type Ⅰ collagen, type Ⅲ collagen and matrix MMP9 in hypertension with left ventricular hypertrophy group were higher than the hypertension group(P＜005).The expression level of serum miRNA29a3p in hypertension with left ventricular hypertrophy was positively correlated with the levels of type Ⅰ collagen, type Ⅲ collagen and matrix MMP9(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Level of miRNA29a3p reflects the degree of myocardial fibrosis in patients with hypertension with left ventricular hypertrophy.
    Logistic regression analysis and countermeasure of nondrug influencing factors on Helicobacter pylori eradication rate
    SU Weixian, DAI Jinyu, SHI Zenghui
    2019, 40(7):  777-780.  doi:10.3969/j.issn.10073205.2019.07.008
    Asbtract ( 576 )   PDF (363KB) ( 181 )  
    Related Articles | Metrics
    〗［Abstract］〓Objective〖HTSS〗〓To analyze the nondrug influencing factors on eradication rate of Helicobacter pylori(H.pylori) and related countermeasures.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Ninetyeight patients with H.pyloripositive peptic ulcer or chronic gastritis were selected. All patients were treated with bismuth quadruple regimen(rabeprazole+bismuth potassium citrate capsule+amoxicillin+clarithromycin) for eradication therapy about 2 weeks. After four weeks at the end of the course of treatment, 14C breath test was reexamined to observe the result of H.pylori eradication. The nondrug factors influencing the eradication rate of H.pylori were analyzed, and then the preventive measures were further summarized.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓H.pylori eradication was successful in 75 out of 98 patients, the eradication rate was 76.5%. The eradication rates of H.pylori in those who did not drink stimulating drinks(tea, coffee), peptic ulcer and body mass index < 25 were higher than those who drank stimulating drinks(tea, coffee), chronic gastritis and body mass index(≥25), there were statistical significance(P＜005).Logistic regression analysis showed that drinking stimulating drinks, chronic gastritis and body mass index(≥25) were risk factors for H.pylori eradication rate.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The eradication rate of H.pylori can be improved by controlling the consumption of stimulating drinks, curing chronic gastritis, prolonging the course of treatment about overweight or obese patients, and appropriately increasing the dosage of drugs.
    Effect of drugcoated balloon on diabetic patients with inferior genu artery disease#br#
    YAO Huaqiang1, BAI Jundong2, ZHANG Yuna3, CHEN Xianghong1, LI Yanmin4, WANG Fujun3*
    2019, 40(7):  781-784,788.  doi:10.3969/j.issn.10073205.2019.07.009
    Asbtract ( 557 )   PDF (408KB) ( 220 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To investigate the effect of drugcoated balloon on diabetic patients with inferior genu artery disease.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Sixtyfour diabetic patients with inferior genu artery disease were randomly divided into observation group and control group with 32 cases in each group. The control group was treated with percutaneous translumin alangioplasty and the observation group with drugcoated balloon. The primary patency rate, the incidence of revascularization and restenosis were compared between two groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓After treatment, the minimum lumen diameter was significantly increased than that before treatment, and the rate of lumen stenosis was lower than that before treatment in both group(P＜005). There was no significant difference in the minimum lumen diameter and the rate of lumen stenosis between two groups(P＞005). There was no significant difference in operation time, maximal dilation pressure, dilation time, stretch time, dosage of contrast medium and primary patency rate between two groups(P＞005). One year after treatment, the incidence of restenosis in the observation group was significantly lower than that in the control group, and the anklebrachial index was significantly higher than that in the control group(P＜005). There was no significant difference in the rate of revascularization at the target lesions between two groups(P＞005). The improvement rate of walking disorder before discharge and 6minute walking test distance in the observation group were significantly higher than those in the control group(P＜0.05). There was no significant difference in the incidence of adverse reactions between two groups(P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Drugcoated balloon has a better effect on revascularization of target lesions in patients with diabetic inferior genicular artery disease, which can increase their motor ability and improve their quality of life.
    Effect of Shenmai injection on sepsis patients with myocardial injury#br#
    HE Cong1, LONG Ling1, DU Quansheng1, FENG Jing2, LIU Chunxia1, WANG Xianlei3*
    2019, 40(7):  785-788.  doi:10.3969/j.issn.10073205.2019.07.010
    Asbtract ( 681 )   PDF (370KB) ( 218 )  
    Related Articles | Metrics
    ［Abstract］ Objective〖HTSS〗〓To investigate the cardioprotective effect of Shenmai injection in patients with sepsis and myocardial injury.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirtytwo patients with myocardial injury due to sepsis were selected. All patients underwent invasive hemodynamic monitoring and ultrasound monitoring to guide fluid resuscitation. All patients were given standard conventional bundle therapy(antiinfection, respiratory and circulatory support, etc.).32 patients were randomly divided into 2 groups,16 cases per group. The control group: standard bundle treatment.Treatment group: standardized bundle therapy + Shenmai injection 200 mL, once daily for 7 days.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓There was no significant difference in brain natriuretic peptide(BNP) level and troponin I(cTnI) level between two groups on day 1 and day 4(P＞005). The level of BNP and cTnI in the treatment group on day 7 was higher than that in the control group, and the difference was statistically significant(P＜005). The creatine kinase isoenzyme MB(CKMB) level in both groups decreased with time, but the level of CKMB in the two groups on day 1, 4 and 7 decreased.There was no statistically significant difference in the improvement rate of ECG between two groups(P＞005). There was no statistically significant difference in the length of ICU stay and 28day mortality between two groups(P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Shenmai injection can protect the myocardium of patients with sepsis myocardial injury.
    miR122 inhibits the activity of liver cancer cells by targeting the LMNB2 gene
    YANG Tao, LI Xiaona
    2019, 40(7):  789-793.  doi:10.3969/j.issn.10073205.2019.07.011
    Asbtract ( 649 )   PDF (384KB) ( 123 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To investigate the role and mechanism of miR122 in the progression of liver cancer.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The regulatory effect of miR122 on LMNB2 gene was confirmed by luciferase reporter gene assay and Western blot. Then the effect of LMNB2 gene on the bioactivity of hepatocellular carcinoma（HCC） cells was analyzed by colony formation assay and transwell cell invasion assay .
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The luciferase activity of the wildtype mir122 mimics was lower than that of the control group(P＜005). The luciferase activity of the mutant transfected with mir122 mimics was not significantly different from that of the control group(P＞005). The expression of mir122 was silenced in hepatocellular carcinoma cells Hep3B, and the expression level of LMNB2 was significantly higher than that of the control group(P＜005). LMNB2 expression level was significantly lower than that of the control group after overexpression of mir122 in liver cancer SMMC7721 cells(P＜005). LMNB2 was overexpressed in hepatocellular carcinoma cells Hep3B, and the invasion and migration ability of hepatocellular carcinoma cells was significantly stronger than that of the control group(P＜005). The LMNB2 expression was silenced in SMMC7721 HCC cells, and the invasion and migration ability of HCC cells was significantly lower than that of the control group(P＜005). LMNB2 was overexpressed in hepatocellular carcinoma cells Hep3B, and the proliferation capacity of hepatocellular carcinoma cells was significantly stronger than that of the control group(P＜005). The LMNB2 expression was silenced in SMMC7721 hepatocellular carcinoma cells, and the proliferation capacity of hepatocellular carcinoma cells was significantly lower than that of the control group(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓miR122 can inhibit the biological activity of HCC cells by targeting LMNB2, which can be used as an early diagnostic indicator and therapeutic target for HCC.
    Deformationbased imaging classification of medial compartment knee osteoarthritis and its clinical significance#br#
    LU Yang1，2， ZHOU Huilin1， HAO Ruizheng3， ZHENG Zhanle1， LYU Hongzhi1， ZHANG Yingze1*
    2019, 40(7):  794-798.  doi:10.3969/j.issn.10073205.2019.07.012
    Asbtract ( 559 )   PDF (630KB) ( 305 )  
    Related Articles | Metrics
    ［Abstract］ Objective〖HTSS〗〓To investigate the feasibility of genu varum and its clinical significance based on morphological changes of femur and tibia.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Radiographic images of 1 422 knees of varus were collected and analyzed. The alignments were drawn and multiple parameters of lower extremities were measured. The images were classed into four types on the basis of mechanical medial distal femoral angle(mMDFA）, medial proximal tibial angle(MPTA） and joint line convergence angle(JLCA). The different between the distributions of different genders was compared.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Knee varus can be divided into four types with 72.36%（1 029/1 422） of type Ⅰ, 1.05%(15/1 422) of typeⅡ, 23.56%(335/1 422) of type Ⅲ and 3.02%(43/1422) of type Ⅳ. There is no significant difference between men and women. 
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓he typing method could fully reflect the morphological changes of the entire lowerlimb for the change of femur, and could provide a theoretical basis for the kneesalvage treatment or total knee arthroplasty.
    The clinical feasibility study on surgical laparoscopic appendectomy combined with transperitoneal anterior inguinal hernia repair #br#
    LI Chao, WANG Hong, LIU Yuhai, LI Haiwen, DAI Fu
    2019, 40(7):  799-802.  doi:10.3969/j.issn.10073205.2019.07.013
    Asbtract ( 571 )   PDF (383KB) ( 98 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To investigate the clinical feasibility and safety of laparoscopic appendectomy (LA) combined with Transabdominal preperitoneal repair(TAPP) at the same time.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Totally 13 patients with acute appendicitis and inguinal hernia in our department were enrolled in this study. All of them were treated with LA combined with TAPP surgery. The concept of enhanced recovery after surgery was applied to guide the perioperative management, and then, postoperative recovery and the incidence rate of recent postoperative complications including urinary retention, early inflammatory bowel obstruction, bleeding, residual appendicitis, incision infection, and inguinal hernia recurrence one year after surgery were observed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓All the 13 patients successfully completed the operation. All the patients had no complications after surgery such as incision infection, hernia patch infection, and inguinal hernia recurrence. Only 1 patient had postoperative urinary retention, and 1 patient had early inflammatory bowel obstruction, both of them successfully discharged after treatment.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The surgery LA combined with TAPP is feasible and safe for strict selected patients who have both acute appendicitis and inguinal hernia.
    Liver function score in patients with portal vein thrombosis after splenectomy and pericardial devascularization#br#
    GONG Shaojun
    2019, 40(7):  803-806.  doi:10.3969/j.issn.10073205.2019.07.014
    Asbtract ( 591 )   PDF (351KB) ( 81 )  
    Related Articles | Metrics
    ［Abstract］ Objective〖HTSS〗〓To investigate the causes of portal vein thrombosis and the influence of liver function scores in patients with splenectomy and pericardial devascularization.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Eighty patients with cirrhotic portal hypertension due to cirrhosis who underwent splenectomy and cardiac vascularization were selected. All patients′ liver function scores and other relevant data before and after surgery were retrospectively analyzed, and the possible causes of portal vein thrombosis were discussed and analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓After surgery, the levels of cholinesterase, total bilirubin and Child scores were significantly lower than those before surgery, and the difference was statistically significant(P＜005). After surgery, there were no significant differences in albumin, prothrombin time, cholinesterase, total bilirubin and Child score between two groups(P＞005). Ascites complication, surgical removal of spleen mass, postoperative red blood cell count, postoperative dimer level, postoperative portal vein diameter were higher or higher than nonthrombotic group, and postoperative portal vein velocity was lower than nonthrombotic group （P＜005）. Logistic regression analysis was performed with thrombosis as the dependent variable, postoperative ascites, surgical removal of spleen mass, postoperative red blood cell count, postoperative dimer, postoperative portal vein diameter, and postoperative portal vein velocity as independent variables. Increased postoperative dimer, increased postoperative portal vein diameter, and slow postoperative portal vein flowere risk factors for thrombosis.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The liver function of patients with splenectomy and pericardial devascularization is improved. The postoperative dimer level should be closely observed. The postoperative portal vein diameter and postoperative portal vein velocity should be detected to prevent thrombosis in the portal vein.
    Clinical effect of carprostol tromethamine in patients with postpartum hemorrhage and its effect on FIB and DD#br#
    XIONG Ying, MA Honglian， LUO Hongxia*
    2019, 40(7):  807-809，814.  doi:10.3969/j.issn.10073205.2019.07.015
    Asbtract ( 510 )   PDF (375KB) ( 175 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To investigate the clinical effects of carprostol tromethamine in patients with postpartum hemorrhage and the effects on fibrinogen(FIB) and Ddimer(DD) levels.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 78 patients with postpartum hemorrhage were enrolled. The patients were randomly divided into study group and control group by weighting method, with 39 cases in each group. The control group was treated with oxytocin, and the study group was treated with carprost tromethamine. The therapeutic effects, FIB and DD levels, postpartum hemorrhage, duration of lochia, rate of decline of the uterus, and uterine contraction time were compared between the two groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The therapeutic effect of the study group is better than that of the control group(P＜005). After treatment, the levels of FIB and DD in the two groups were lower than those before treatment, and the levels of FIB and DD in the study group were lower than those in the control group(P＜005). The bleeding volume of the study group at 1 h and 12 h after birth was less than that of the control group. The uterine contraction time was longer than that of the control group. The uterus bottom decreased faster than the control group, and the duration of lochia was shorter than that of the control group(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Carbaryl tromethamine is effective in the treatment of postpartum hemorrhage, which improves clinical symptoms, reduces the level of FIB and DD, improves the quality of life, and has higher safety, which can be widely applied in clinical practice.
    Expression and clinical significance of Rab5 and Smad2 in endometrium of patients with endometriosis
    LIN Huifang， ZHANG Jing， SUN Donglan， ZHANG Jing*
    2019, 40(7):  810-814.  doi:10.3969/j.issn.10073205.2019.07.016
    Asbtract ( 519 )   PDF (529KB) ( 124 )  
    Related Articles | Metrics
    ［Abstract］ Objective〖HTSS〗〓To study the expression of Rab5 and Smad2 in endometrial tissues of patients with endometriosis and analyze their clinical significance.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 364 patients treated with laparoscopic surgery were selected as the research objects. Among them, 182 patients diagnosed with endometriosis after laparoscopic surgery were taken as the observation group, and 182 patients with uterine fibroids admitted to our hospital during the same period were taken as the control group. The expressions of serum CA125, inflammatory factors and serum endometrial antibodies were compared between these two groups. The above indexes of different rAFS staging groups were compared. Western blot was used to detect the expression of Rab5 and Smad2 in tissue. The patients′ clinical indicators were observed and their correlation with Rab5 and Smad2 was analyzed to make the receiver operating characteristic curve.  Logistic multiple regression analysis of related factors of endometriosis.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The levels of CA125, inflammatory factors and serum endometrial antibodies in the observation group were significantly higher than those in the control group in serum. The expression of Rab5 and Smad2 in the observation group was significantly higher than that in the control group(P＜005). The expression of Rab5 and Smad2 was positively correlated with CA125, inflammatory factors and serum endometrial antibodies(P＜005). Logistic multiple regression analysis showed that Rab5 and Smad2 in endometrial tissues were risk factors for patients with endometriosis.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The expression of Rab5 and Smad2 in endometrial tissues of patients with endometriosis is significantly increased, which is positively correlated with the corresponding clinical indicators and is a risk factor for endometriosis.
    The effect of PCEA combined with intermittent epidural bolus analgesia on T lymphocyte subsets and motor functions in pregnant women#br#
    ZHANG Jing1， XUE Ning1， LI Shengnan2， WANG Xiufang1， CHEN Jianghong3， YANG Shanshan4
    2019, 40(7):  815-819，824.  doi:10.3969/j.issn.10073205.2019.07.017
    Asbtract ( 463 )   PDF (445KB) ( 139 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To explore the effect of patient control epidural analgesia(PCEA) combined with intermittent epidural bolus(IEB) analgesia on T lymphocyte subsets and motor functions in pregnant women.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The 140 cases of fullterm advanced maternal age were selected. According to the random number table method, they were divided into observation group(n=70) and control group(n=70). The control group was anesthetized with PCEA combined with continuous epidural infusion(CEI), the observation group was anesthetized with PCEA combined with IEB. Compared the analgesic effects, indexes of intraoperative and postoperative operation in parturient, motion block, T lymphocyte subsets and inflammatory factor levels before and after anesthesia in both groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The Visual Analogue Scale(VAS) scores of both groups decreased first and then increased. The VAS score of the observation group was significantly lower than that of the control group, the difference of interblock, time points and interblock·time points was statistically significant（P＜005）. The number of PCEA addition, the dosage of ropivacaine, sufentanil and caesarean section cases in the observation group were less than those in the control group, and the maternal satisfaction in the observation group was higher than that of the control group（P＜005）. The incidence of motor block in the observation group was significantly lower than that in the control group(P＜005). There was no significant difference between the two groups on the first, second and thirdstage of labor time, analgesic time, interval and duration timeofuterine contraction, fetal heart rate, the number of patients using oxytocin，pregnant women adverse reactions, neonate Apgar scores 1 and 5 minutes after birth（P＞005）. The levels of CD3+, CD4+, CD4+/CD8+, interleukin 6(IL6) and tumor necrosis factor α(TNFα) had the trend of decrease in both groups. The CD3+, CD4+ and CD4+/CD8+ of the observation group were higher than those of the control group, and IL6 and TNFα were lower than those of the control group, the difference of interblock, time points and interblock·time points was statistically significant（P＜005）. The difference of interblock, time points and interblock·time points on CD8+ in the two groups was not statistically significant（P＞005）.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The analgesic effect of PCEA combined with IEB on aged parturient was significant, the dosage of anesthetic was less, the patient satisfaction was high, it can reduce the rate of cesarean section and the occurrence of motor block, and improve the immune suppression and inflammation, which is worth popularizing.
    Clinical effect of terbutaline combined with azithromycin in the treatment of mycoplasma pneumoniae pneumonia
    WANG Zhikun， WANG Jianzhong， LI Tong， HAN Jinlin
    2019, 40(7):  820-824.  doi:10.3969/j.issn.10073205.2019.07.018
    Asbtract ( 638 )   PDF (424KB) ( 89 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To observe the clinical effect of terbutaline combined with azithromycin in the treatment of mycoplasma pneumoniae pneumonia(MPP).
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 116 cases of children with MPP were selected as the research object in our hospital. The patients were randomly divided into observation group and control group with 58 cases in each group. Patients in both groups were given azithromycin sequential therapy, and patients in the observation group were given terbutaline sulfate as well. The extinction time of symptoms and signs, the length of hospital stay, the clinical curative effect, the adverse reactions and the levels of interleukin6(IL6), tumor necrosis factorα(TNFα), Creactive protein(CRP), granulocytemacrophage colony stimulating factor(GMCSF) and CXC chemokine ligand 8(CXCL8) were compared between the two groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The extinction time of symptoms and signs and the time of hospitalization of the observation group were significantly shorter than that of the control group, and the difference was statistically significant(P＜005). The clinical efficacy of the observation group was better than that of the control group, and the total effective rate was higher than that of the control group(P＜005). After treatment, the levels of GMCSF, CXCL8, IL6, TNFa and CRP in the two groups were lower than those before treatment, and the levels of GMCSF, CXCL8, IL6, TNFα and CRP in the observation group were lower than those of the control group(P＜005). There was no significant difference in the incidence of adverse reactions between the two groups(P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Terbutaline combined with Azithromycin sequential therapy in the treatment of MPP in children has synergistic effect. It can inhibit inflammation, reduce the expression levels of GMCSF, CXCL8 and related inflammatory factors, improve bronchial stenosis, reduce airway hyperresponsiveness, alleviate clinical symptoms and shorten treatment time. It is worthy of clinical application.
    Comparison of vitamin D status in children with newly diagnosed Hashimoto thyroiditis and Graves disease in Han nationality in Shijiazhuang City#br#
    PI Yalei1, ZHANG Yanan1, HAN Xiao1, GAO Liping2, ZHANG Huifeng1, YAN Xue1*
    2019, 40(7):  825-828，833.  doi:10.3969/j.issn.10073205.2019.07.019
    Asbtract ( 605 )   PDF (392KB) ( 101 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To compare the vitamin D status in children with Hashimoto thyroiditis(HT) and Graves disease(GD)，and to explore the role of vitamin D in the internal relationship between the two diseases.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Forty cases with HT and 37 cases with GD were collected from Shijiazhuang Han nationality. Four milliliter fasting venous blood samples of all the patients were collected in the morning to determine 25 hydroxyvitamin D(25OHD), thyroid stimulating hormone(TSH), total thyroxine(TT4), total triiodothyronine(TT3), free thyroxine(FT4), free triiodothyronine(FT3), thyrotropin receptor antibody(TRAb), thyroid globulin antibody(TGAb) and thyroid peroxidase antibody(TPOAb). The lengths, widths and thicknesses of the left and right lobes of the thyroids were measured by ultrasound, and the thyroids volume were calculated. The iodine of the middle part urine in the morning was measured by electrochemical method.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The levels of FT3, FT4, TT3, TT4 and TRAb in HT group were significantly lower than that of GD group, TSH and TGAb were significantly higher than those in GD group(P＜005). There were no significant differences in 25OHD and TPOAb levels between the 2 groups(P＞005). TGAb in HT group with thyroid volume was significantly higher than that in GD group, and TRAb was significantly lower than GD group(P＜005). There was no significant difference in thyroid volume, 25OHD and TPOAb between the two groups(P＞005). TGAb in HT group with the results of urine iodine was significantly higher than that in GD group, and TRAb was significantly lower than that in GD group(P＜005). There was no significant difference in urinary iodine, 25OHD and TPOAb between the two groups(P＞005). There was no correlation between 25OHD level and TSH, TRAb, TGAb, and TPOAb in HT group(P＞005). There was a weak positive correlation between 25OHD level and TPOAb in GD group(P＜005), but no correlation with TSH, TRAb and TGAb(P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓There is no significant difference in serum 25OHD levels between children with HT and GD. Serum 25OHD level may not play a role in the internal relationship between the twodiseases.
    Comparative study of serological and molecular biological methods for detection of pediatric Mycoplasma pneumoniae infection
     
    FENG Zhishan1, LI Guixia1,2, GUO Yinghui2, YANG Shuo1, YAN Xiaotong1, WANG Le1*
    2019, 40(7):  829-833.  doi:10.3969/j.issn.10073205.2019.07.020
    Asbtract ( 810 )   PDF (398KB) ( 172 )  
    Related Articles | Metrics
    ［Abstract］ Objective〖HTSS〗〓To compare the serological and molecular biological tests for detecting the infection of pediatric Mycoplasma pneumoniae and to analyze the causes of difference.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓9 339 serum samples from children with suspected respiratory tract infection were selected continuously. The patients were aged 0-13 years old. Serum and respiratory secretions from these patients were collected. The antibodies against M. pneumoniae were detected by passive agglutination, the nucleic acids of M. pneumoniae in respiratory secretions were amplified by multiplex PCR or simultaneous amplificationmethods.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓After excluding 428 second sera without altered titer, a total of 8 911 blood samples and 4 045 secretion samples were collected. Among the 8 911 specimens tested by serology assay, the detection rate of female was higher than that of males. The older the age, the higher the positive rate of antibody detection. The positive rate in outpatients was higher than that in hospitalized ones, and the positive rate was greater in spring and summer season. In the 4 065 specimens of nucleic acid detection, the older the age, the greater the positive rate.The positive rate of nucleic acid detection in autumn and winter was greater than that in spring and summer(P＜005), but the significance was not observed in genders and hospitalization. We analyzed the antibody titer and found that in outpatients, the proportion of 1〖DK〗∶320 titers was significantly higher than other titers(14.7%,1 310/8 911). Using nucleic acid detection as gold standard, the results of antibody titer of 1〖DK〗∶160 and 1〖DK〗∶640 were evaluated. When the 1〖DK〗∶640 was set as positive, the consistency was better than that of 1〖DK〗∶160, although the sensitivity decreased, the specificity, accuracy and positive predictive value were all greater than that of 1〖DK〗∶160.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓There were difference between antibody and nucleic acid detection methods. In outpatients or when the serum titer is 1〖DK〗∶320, two methods should be combined to determine the infection of M. pneumoniae.
    Therapeutic effect of ranibizumab combined with 577 nm laser photocoagulation on severe nonproliferative diabetic retinopathy with macular edema#br#
    CHEN Jiali, LI Wei, LI Lin*
    2019, 40(7):  834-837.  doi:10.3969/j.issn.10073205.2019.07.021
    Asbtract ( 616 )   PDF (355KB) ( 114 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To observe the clinical efficacy of ranibizumab combined with 577 nm laser photocoagulation for severe nonproliferative diabetic retinopathy with macular edema.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓 Sixty patients and 60 eyes with severe nonproliferative diabetic retinopathy with macular edema were randomly divided into observation group and control group, 30 cases and 30 eyes respectively. The observation group was given ranibizumab in combination with 577 nm laser photocoagulation, and the control group was given 577 nm laser photocoagulation. Optical coherence tomography and fluorescein fundus angiography were reexamined 1 week, 1 month, 3 months and 6 months after treatment. The best corrected visual acuity before and after treatment, the thickness of the foveal retinal neuroepithelial foci of the macula and the total volume of the neuroepithelial lesion of the 6 mm diameter in the macular area were recorded and compared. The laser energy, energy density, number of spots and complications were compared between the two groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓With the extension of time, the best corrected visual acuity of two groups were significantly increased, the macular foveal retinal neuroepithelial thickness and macular area 6 mm diameter neuroepithelial overall volume of two groups were significantly decreased. The best corrected vision of observation group was higher than the control group, macular foveal retinal neuroepithelial thickness and macular area 6 mm diameter neuroepithelial overall volume were lower than the control group, There were significant differences in interaction among groups, time points and time points between groups（P＜005）. The laser energy, spot number and energy density of the observation group were lower than that of the control group （P＜005）. No serious complications occurred in either group. 〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The efficacy of ranibizumab combined with 577 nm laser photocoagulation for severe nonproliferative diabetic retinopathy with macular edema is positive and clinically safe.
    Quantitative application of high frequency ultrasound in monitoring the curative effect of rheumatoid arthritis#br#
    ZHUANG Yu， LYU Zhifen， ZHANG Xuezhen， CHEN Yuhang， WANG Zhiwen， TAN Zhiming*
    2019, 40(7):  838-841.  doi:10.3969/j.issn.10073205.2019.07.022
    Asbtract ( 627 )   PDF (344KB) ( 164 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To investigate the quantitative application of high frequency ultrasound in monitoring the curative effect of rheumatoid arthritis. 
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Sixty patients with RA were examined by highfrequency ultrasound before treatment, during remission and half a year after remission. Synovial thickness, gray scale(GS) and power Doppler(PD) scores of seven specific joints were observed. Erythrocyte sedimentation rate, Creactive protein and rheumatoid factor levels were measured. The correlation between synovial changes and inflammatory indexes was analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with 840 joint ultrasound images of 60 cases of RA patients, synovial membrane thickness and GS score, blood flow PD score, erythrocyte sedimentation rate, Creactive protein and rheumatoid factor levels of 7 joints in remission period after treatment and half a year after remission were significantly lower than or lower than those before treatment. Six months after remission, synovial membrane thickness and GS score, PD score, erythrocyte sedimentation rate, Creactive protein and rheumatoid factor of 7 joints were significantly lower than those before treatment. The levels were significantly lower than or below the remission stage(P＜005). The synovial membrane thickness and synovial blood flow were positively correlated with erythrocyte sedimentation rate, Creactive protein and rheumatoid factor(P＜005). 
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓High frequency ultrasound shows definite significance in the monitoring of rheumatoid arthritis. Simplified GS and PD quantitative scores of 7 joints are positively correlated with inflammation index and rheumatoid factor titer. It has important guiding significance for formulating and adjusting the therapeutic scheme of rheumatoid therapy.
    Effects of Ulinastatin on postoperative cognitive dysfunction and related mechanisms in rats after surgical trauma#br#
    DU Yiri1， LIU Bin2, LANG Haiyun1， LI Changsheng1， LI Hongbin1， XIE Yaying1*
    2019, 40(7):  842-846.  doi:10.3969/j.issn.10073205.2019.07.023
    Asbtract ( 579 )   PDF (400KB) ( 74 )  
    Related Articles | Metrics
    ［Abstract］〓Objective〖HTSS〗〓To observe the protective effect of ulinastatin on the postoperative cognitive dysfunction(POCD) impairment and to explore its specific mechanism of action in the pathogenesis of disease.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Wistar rats were randomly divided into control group, POCD group, ulinastatin group, preoperative 5 days of space acquired experimental study. The control group was injected with normal saline; the POCD group was injected with normal saline after splenectomy under general anesthesia; and the ulinastatin group was injected with ulinastatin before and after the preparation of POCD model. The behavioral changes of rats in each group were evaluated 3 and 7 days after operation. The contents of malondialdehyde(MDA), superoxide dismutase(SOD), tumor necrosis factorα(TNFα), interleukin1β(IL1β) and nuclear factorκB(NFκB) in hippocampus and prefrontal cortex were measured.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Three days after operation, the rats in POCD group had longer residence time, fewer times to cross the platform, longer working memory latency,  higher MDA content and lower SOD content than those in the control group. The levels of TNFa, IL1β and NFκB in POCD group were higher than those in the control group（P＜0.05）. The retention time of rats in ulinastatin group was shorter than that in control group and longer than that in POCD group. The rats in ulinastatin group had more times of perforation, longer working memory latency, shorter working memory latency than POCD group. The rats in ulinastatin group had higher MDA content than control group, lower SOD content than control group, higher SOD content than POCD group. The rats in ulinastatin group had higher TNFa, IL1β and NFκB levels than control group, had lower those than POCD group(P＜005). At 7 days after surgery, the residence time of the control group and the ulinastatin group was shorter than that at the 3 days after surgery, and the working memory latency was longer than that at the 3 days after surgery. At 7 days after surgery, the MDA content in the control group and ulinastatin group was higher than that in the 3 days after surgery，the MDA content in the POCD group was lower than that at the 3 days after surgery. At 7 days after surgery, the SOD content of the control group was lower than that at the 3 days after surgery， the MDA content in the ulinastatin group of the POCD group was higher than that of the postoperative 3 days. At 7 days after surgery, the levels of TNFα in the POCD group and ulinastatin group were lower than those at 3 days after surgery，the levels of IL1β and NFκB in the three groups were lower than those in the 3 days after surgery(P＜005).The residence time of the rats in the POCD group was shorter than that of the control group, and the latency of working memory was longer than that of the control group. The residence time of the rats in the ulinastatin group was shorter than that of the control group and longer than that of the POCD group. The working memory latency was longer than that of the control group and shorter than the POCD group. The MDA content in the POCD group was higher than that in the control group, and the SOD content was lower than that in the control group. The MDA content in the ulinastatin group was higher than that in the control group, and the SOD content was lower than that in the control group, which was higher than the POCD group. The levels of TNFα, IL1β and NFκB in the POCD group were higher than those in the control group. The level of IL1β in the ulinastatin group was lower than that in the POCD group(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Ulinastatin has a positive effect on improving postoperative cognitive dysfunction by scavenging oxygen free radicals and inhibiting inflammatory responses.