白藜芦醇对糖尿病大鼠视网膜病变的影响#br#

doi:10.3969/j.issn.10073205.2017.11.016

河北医科大学学报

白藜芦醇对糖尿病大鼠视网膜病变的影响#br#

1.唐山爱尔眼科医院眼科，河北唐山 063000;2.冀东眼科医院眼科，河北唐山 063000;
3.华北理工大学附属医院眼科，河北唐山 063000

出版日期:2017-11-25 发布日期:2017-11-16
作者简介:刘俊辉（1978-），女，河北唐山人，唐山爱尔眼科医院主治医师，医学学士，从事眼科疾病诊治研究。

The effects of Resveratrol on diabetic rats with retinopathy#br#

1.Department of Ophthalmology, Tangshan Aier Eye hospital, Hebei Province， Tangshan 063000, China;
2.Department of Ophthalmology, Jidong Ophthalmology Hospital, Hebei Province， Tangshan
063000, China; 3.Department of Ophthalmology, North China University of Science and
Technology Affiliated Hospital， Hebei Province, Tangshan 063000, China

Online:2017-11-25 Published:2017-11-16

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗观察白藜芦醇（Resveratrol，RES）对糖尿病大鼠视网膜病变的影响并探讨相关机制。
〖HTH〗方法〖HTSS〗〖KG*2〗清洁级健康雄性SD大鼠45只随机分为对照组、模型组和RES组各15只。应用链脲佐菌素腹腔注射制作糖尿病大鼠模型。造模后1周，RES组大鼠给予白藜芦醇40 mg/kg（10 mL/kg）腹腔注射治疗，1次/d，连续治疗6周。治疗结束后，测量各组大鼠血糖、体质量，采用酶联免疫吸附测定法检测大鼠视网膜中肿瘤坏死因子α（tumor necrosis factorα，TNFα）和白细胞介素1β（interleukin1β，IL1β）含量，检测大鼠血清中血管内皮生长因子（vascular endothelial growth factor，VEGF）、缺氧诱导因子1α（hypoxia inducible factor1α，HIF1α）、丙二醛（methane dicarboxylic aldehyde，MDA）和超氧化物歧化酶（superoxide dismutase，SOD）含量。
〖HTH〗结果〖HTSS〗〖KG*2〗模型组和RES组血糖高于对照组，体质量低于对照组（P＜005）；模型组与RES组血糖、体质量差异均无统计学意义（P＞005）。模型组和RES组大鼠TNFα、IL1β、VEGF、HIF1α和MDA表达明显高于对照组，RES组大鼠TNFα、IL1β、VEGF、HIF1α和MDA表达低于模型组，差异均有统计学意义（P＜005）。模型组和RES组大鼠SOD表达明显低于对照组，RES组大鼠SOD表达高于模型组，差异均有统计学意义（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗RES可抑制炎症反应和HIF1α/VEGF通路，减轻氧化应激损伤，从而改善糖尿病视网膜的血管病变。

关键词: 糖尿病视网膜病变, 白藜芦醇, 大鼠, 链脲佐菌素

Abstract: ［Abstract］ Objective〖HTSS〗〓To observe the effects of Resveratrol(RES) on diabetic rats with retinopathy, and to explore its mechanism.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fortyfive cleaning degree healthy male SD rats were randomly divided into 3 groups, control group,model group and RES group(n=15/group). The diabetic rats model were established by streptozotocin intraperitoneal injection. One week after modeling, rats in RES group was administrated intraperitoneally with RES(40 mg/kg, 10 mL/kg) once daily for consecutive 6 weeks. After treatment, the blood glucose and body weight of different groups were measured. The expression of tumor necrosis factor alpha(TNFα) and interleukin1β(IL1β） in retina of different groups were detected by enzyme linked immunosorbent assay(ELISA). The expression of vascular endothelial growth factor(VEGF), hypoxia inducible factor1α(HIF1α), methane dicarboxylic aldehyde(MDA) and superoxide dismutase(SOD) in serum of different groups were detected by ELISA.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The blood glucose of model group and RES group was higher than control group, body weight of model group and RES group was lower than control group(P<0.05), there were no significantly difference between model group and RES group(P>0.05). The expressions of TNFα, IL1β, VEGF, HIF1α and MDA in model group and RES group were higher than that of control group. The e expressions of TNFα, IL1β, VEGF, HIF1α and MDA in RES group were lower than that of model group(P<0.05). The expression of SOD in model group and RES group was lower than that of control group. The expression of SOD in RES group was higher than that of model group(P<0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓RES could inhibit inflammatory reaction, suppresses HIF1α/VEGF pathway and reduces oxidative stress injury, then ameliorates vascular lesions in diabetic retina.

Key words: diabetic retinopathy, Resveratrol, rats, streptozotocin

刘俊辉1,2,李春江2,李玉涛2,3*. 白藜芦醇对糖尿病大鼠视网膜病变的影响#br#[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2017.11.016.

LIU Junhui1,2, LI Chunjiang2, LI Yutao2,3*. The effects of Resveratrol on diabetic rats with retinopathy#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2017.11.016.

[1]	李雪景, 陈冬梅, 段佳良, 姚一民, 尚庆丽. 联合手术治疗增殖性糖尿病视网膜病变的继发性闭角型新生血管性青光眼的临床效果观察 [J]. 河北医科大学学报, 2023, 44(3): 319-323.
[2]	吴苏敏, 周欣, 王丽波. 血清CMKLR1、FSTL1水平与糖尿病视网膜病变严重程度相关性 [J]. 河北医科大学学报, 2023, 44(2): 189-193,213.
[3]	吴海峰, 贾建雷, 李倩, 甄秀丽, 魏雅楠, 乜照燕. 白藜芦醇通过调节SIRT1改善环磷酰胺诱导的人颗粒细胞氧化应激损伤 [J]. 河北医科大学学报, 2022, 43(7): 832-838.
[4]	李爽, 张海召, 姜晓丹, 蒋燕铌. 玻璃体腔注射阿柏西普联合全视网膜光凝治疗糖尿病视网膜病变增殖期黄斑水肿的疗效分析[J]. 河北医科大学学报, 2022, 43(2): 183-186,197.
[5]	王艳新, 贾冠美, 曹朗, 曹顺义, 张士宏. 血浆miR-let-7e-5p、LncRNA NEAT1表达水平与2型糖尿病视网膜病变的关系 [J]. 河北医科大学学报, 2022, 43(10): 1167-1171.
[6]	刘涛, 张望, 钟艳丽, 吴宏伟. MicroRNA-326通过靶向Klk7调控MAPK信号通路对抑郁模型大鼠的神经保护作用[J]. 河北医科大学学报, 2021, 42(6): 631-636,645.
[7]	白洁, 宋妮娟. 基于聚焦解决模式的强化护理对糖尿病性视网膜病变患者负性情绪、依从性及视功能的影响[J]. 河北医科大学学报, 2021, 42(11): 1298-1301,1306.
[8]	刘慧（综述）, 刘丹岩（审校）. 增殖性糖尿病视网膜病变玻璃体切除术后发生新生血管性青光眼的影响因素[J]. 河北医科大学学报, 2021, 42(11): 1357-1361.
[9]	闫姗姗, 赵芳, 肖剑, 张春良. 白藜芦醇在慢性阻塞性肺疾病合并肺源性心脏病SD大鼠肺功能及心脏功能改善中的作用[J]. 河北医科大学学报, 2020, 41(8): 882-887.
[10]	王素星, 邵伟华, 王娜, 吕彩霞. 限食后重饲大鼠躯干脂肪改变对骨密度的影响及机制[J]. 河北医科大学学报, 2020, 41(12): 1374-1378.
[11]	窦方方, 李振通, 曹素平, 邓志峰. 原花青素调控miR-21表达对糖尿病视网膜病变大鼠血管内皮细胞功能及视网膜神经节细胞的保护机制研究[J]. 河北医科大学学报, 2020, 41(12): 1379-1383,1388.
[12]	黄玉哲1，刘丽霞2，邱雨童3，金〓铂4，尹立勇5*. 红景天苷对慢性肾衰竭大鼠保护作用机制的研究#br#[J]. 河北医科大学学报, 2019, 40(8): 882-885,910.
[13]	陈伽俐，李〓炜，李〓林*. 雷珠单抗联合577 nm激光光凝治疗重度非增生型糖尿病视网膜病变伴黄斑水肿的效果观察[J]. 河北医科大学学报, 2019, 40(7): 834-837.
[14]	都义日1，刘〓斌2，郎海云1，李常胜1，李鸿斌1，解雅英1*. 乌司他丁对手术创伤致大鼠术后认知功能障碍的影响及相关机制研究[J]. 河北医科大学学报, 2019, 40(7): 842-846.
[15]	张莉莉1，李玉坤2*. 大鼠骨组织中IRS2 mRNA水平与2型糖尿病性骨质疏松发病的关系[J]. 河北医科大学学报, 2019, 40(6): 637-641.

白藜芦醇对糖尿病大鼠视网膜病变的影响#br#

The effects of Resveratrol on diabetic rats with retinopathy#br#

HTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价