限食后重饲大鼠躯干脂肪改变对骨密度的影响及机制

doi:10.3969/j.issn.1007-3205.2020.12.003

河北医科大学学报 ›› 2020, Vol. 41 ›› Issue (12): 1374-1378.doi: 10.3969/j.issn.1007-3205.2020.12.003

限食后重饲大鼠躯干脂肪改变对骨密度的影响及机制

河北省人民医院老年病二科，河北石家庄 050051

出版日期:2020-12-25 发布日期:2020-12-31
作者简介:王素星（1982-），女，河北新乐人，河北省人民医院主治医师，医学博士，从事内分泌科疾病诊治研究。
基金资助:
河北省卫健委青年科技课题(20180107)；河北省中医药局课题（2018072）；河北省自然科学基金 (H2016307015)

Effect and mechanism of trunk fat changes on bone mineral density in rats re-fed after food restriction

The Second Department of Geriatrics, Hebei General Hospital, Shijiazhuang 050051, China

Online:2020-12-25 Published:2020-12-31

摘要/Abstract

摘要： 目的观察限食后重饲大鼠躯干脂肪的改变情况及对骨密度的影响及可能机制。
方法 8周龄雄性SD大鼠分为正常对照（normal control,NC）组,普通饮食4周、12周（NC4、NC12)组、热卡限制（calorie restriction for 4 weeks,R4）组、限食后普通饮食重饲（refeeding with normal chow,RN）组、限食后高脂饮食重饲 (refeeding with high-fat diet,RH)组。实验4周、12周检测体重，躯干脂肪含量及躯干与全身脂肪比例，全身体重指数，血清胰岛素（fasting insulin,FINS）、游离脂肪酸（free fatty acids,FFA）、炎症因子及骨转换标志物水平。
结果 ①热卡限制后的重饲使躯干脂肪含量及躯干脂肪比例明显高于NC12 组（P＜0.01）。全身骨密度在限食期间呈显著下降趋势（P＜0.05）。恢复饮食后，RN 组BMD恢复程度优于RH 组。②R4组三酰甘油(triglyceride,TG)、肿瘤坏死因子α（tumor necrosisfactor-α,TNF-α）,白细胞介素6（ interleuken-6,IL-6）、骨转换标志物（碱性磷酸酶、骨钙素）水平均明显降低（P＜0.05）；重饲后RH及RN组血清碱性磷酸酶、骨钙素水平有所回升，仍较NC12组低（P＜0.05），而FINS、FFA、TG、TNF-α及IL-6水平显著升高（P＜0.05），尤以RH组为著。③逐步回归分析显示躯干脂肪含量、躯干脂肪比例是影响全身骨密度的重要因素（r2=0.823，P＜0.001）。
结论限食后重饲可导致躯干部脂肪堆积、骨密度下降，以高脂饮食重饲为著；躯干脂肪堆积导致的脂质外溢、炎症因子分泌增多，进而影响骨转换，可能是降低全身骨密度的重要原因。

关键词: 骨密度, 脂肪类, 大鼠

Abstract: Objective To investigate the changes of trunk fat and its relationship with bone mineral density in rats re-fed after calorie restriction and its possible mechanism.
Methods Male SD rats, aged 8 weeks, were divided into normal chow(containing 4-week and 12-week subgroups, NC4 and NC12) group, calorie restriction for 4 weeks(R4) group, refeeding with normal chow after calorie restriction(RN) group, refeeding with high-fat diet after calorie restriction(RH) group. Trunk fat content and the whole body BMD were detected at the end of 4 and 12 weeks, along with body weight, fasting insulin(FINS), free fatty acids(FFA), inflammatory factors and bone turnover markers.
Results (1) Refeeding after calorie restriction increased the trunk fat content and trunk fat ratio significantly(vs.NC12, P＜0.01).The bone mineral density decreased in R4 group(vs.NC4, P＜0.05). After refeeding, the recovery degree of bone mineral density in RN group was higher than that in RH group.(2) The levels of triglyceride(TG), inflammatory factors(tumor necrosisfactor-α and interleuken-6,TNF-α and IL-6), bone turnover markers(alkaline phosphatase, osteocalcin) were significantly decreased in R4 group(P＜0.05). After refeeding, the levels of serum alkaline phosphatase and osteocalcin in RH and RN groups increased, but were still lower than those in NC12 group(P＜0.05),while the levels of FINS, FFA, TG, TNF-α and IL-6 were significantly increased(vs.NC12, P＜0.05), especially in RH group.(3) In the linear regression analysis, trunk fat content and trunk fat ratio were both entered as predictors(r2=0.823， P＜0.001).
Conclusion Refeeding after calorie restriction can lead to trunk fat accumulation and the whole body bone mineral density decrease, especially refeeding with high-fat diet. Lipid spillover and secretion of inflammatory factors increase due to fat accumulation in the trunk, and then affect bone turnover, which may be an important reason for reducing the whole body bone mineral density.

Key words: bone density, fats；rats

王素星, 邵伟华, 王娜, 吕彩霞. 限食后重饲大鼠躯干脂肪改变对骨密度的影响及机制[J]. 河北医科大学学报, 2020, 41(12): 1374-1378.

WANG Su-xing, SHAO Wei-hua, WANG Na, LYU Cai-xia. Effect and mechanism of trunk fat changes on bone mineral density in rats re-fed after food restriction[J]. Journal of Hebei Medical University, 2020, 41(12): 1374-1378.

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限食后重饲大鼠躯干脂肪改变对骨密度的影响及机制

Effect and mechanism of trunk fat changes on bone mineral density in rats re-fed after food restriction

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