GPX-1在大鼠肝缺血再灌注损伤模型的表达变化

doi:10.3969/j.issn.10073205.2019.01.003

河北医科大学学报

GPX-1在大鼠肝缺血再灌注损伤模型的表达变化

1.河北省石家庄市妇幼保健院药剂科，河北石家庄 050051；2.河北省井陉县医院神经内科，河北井陉 050300；3. 河北医科大学基础医学院人体解剖学教研室，河北石家庄 050017

出版日期:2019-12-25 发布日期:2018-12-29
作者简介:于国霞（1977-），女，河北赞皇人，河北省石家庄市妇幼保健院主管中药师，医学学士，从事临床药学研究。
基金资助:
河北省医学科学研究重点课题（20180693）

Expression change of GPX1 in hepatic ischemiareperfusion injury rat model

1.Department of Pharmacy, Shijiazhuang Maternal and Child Health Care Hospital, Hebei Province,
Shijiazhuang 050051, China; 2.Department of Neurology, Jingxing County Hospital, Hebei Province,
Jingxing 050300, China; 3.Department of Anatomy, School of Basic Medical Sciences,
Hebei Medical University, Shijiazhuang 050017， China

Online:2019-12-25 Published:2018-12-29

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗观察谷胱甘肽过氧化物酶1（glutathione peroxidase 1,GPX1)在大鼠肝缺血再灌注损伤（hepatic ischemiareperfusion injury,HIRI）模型的表达变化，探讨GPX1在清除过氧化物、降低缺血再灌注诱发的过氧化损伤中的作用。
〖HTH〗方法〖HTSS〗〖KG*2〗夹闭大鼠肝中、右叶肝蒂30 min，建立大鼠HIRI模型；肝中、右叶再灌注6 h后取血和肝脏。观察血清丙氨酸转氨酶（alanine aminotransferase,ALT）活性变化，采用速率法测定；HE染色法观察大鼠肝脏形态结构变化；观察肝组织过氧化氢（hydrogen peroxide，H2O2）和丙二醛（malondialdehyde,MDA）含量变化，分别采用钼酸比色法和硫代巴比妥酸比色法测定；GPX1的mRNA表达水平和蛋白表达水平分别采用RTPCR和Western blotting法测定；对GPX1表达水平与MDA含量、GPX1表达水平与H2O2含量分别进行相关性分析。
〖HTH〗结果〖HTSS〗〖KG*2〗HIRI模型组大鼠肝组织形态结构受损明显，肝组织内H2O2和MDA含量均升高，血清ALT活性升高；肝组织内GPX1 mRNA表达水平和蛋白表达水平均明显增强，并与肝组织内MDA含量、H2O2含量呈正相关关系。
〖HTH〗结论〖HTSS〗〖KG*2〗大鼠肝缺血再灌注后，肝组织遭受过氧化损伤；抗氧化酶GPX1表达升高可减轻过氧化损伤。

关键词: 再灌注损伤, 谷胱甘肽过氧化物酶, 过氧化氢

Abstract: ［Abstract］ Objective〖HTSS〗〓To observe the expression change of glutathione peroxidase1(GPX1) in rats of hepatic ischemiareperfusion injury(HIRI) model and investigate the role of GPX1 in scavenging peroxide and reducing peroxidative injury induced by ischemia and reperfusion.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The HIRI model of rats was established by blocking the hepatic pedicle of the right and middle lobes for 30 mins. The blood and liver were taken after 6 hours of reperfusion. The activity of serum ALT was detected with rate method, the changes of liver morphology and structure in rats was observed by HE staining, the malondialdehyde（MDA） and hydrogen peroxide(H2O2 ) content in liver were determined by Thiobarbituric acid colorimetric method，and Molybdate colorimetric method respectively. The GPX1 mRNA and protein expression level were 〖JP2〗evaluated by RTPCR and Western blotting respectively, the correlation between the GPX1〖JP〗 expression level and the MDA content, between the GPX1 expression level and the H2O2 content were analyzed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the rats of control group, the morphological structure of liver of HIRI group was significantly damaged, the serum ALT activity, the content of MDA and H2O2 in the liver of HIRI model were significantly increased, the mRNA and protein expression level of GPX1 in liver were also significantly enhanced, There were positive correlation between GPX1 mRNA and MDA, 〖JP2〗between GPX1 pretein and MDA, between GPX1 mRNA and H2O2, between GPX1〖JP〗 pretein and H2O2.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Ischemia and reperfusion causes liver tissue to be in oxidative stress and been subjected to peroxidative damage. Antioxidant enzymes GPX1 may play an antioxidant stress role by scavenging peroxides during the process, and reduce hepatic peroxidative injury induced by ischemiareperfusion.

Key words: reperfusion injury, glutathione peroxidase, hydrogen peroxide

于国霞1，杨俊慧2，霍宏昌1，王〓磊3，王〓切3*. GPX-1在大鼠肝缺血再灌注损伤模型的表达变化[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2019.01.003.

YU Guoxia1, YANG Junhui2, HUO Hongchang1, WANG Lei3, WANG Qie3*. Expression change of GPX1 in hepatic ischemiareperfusion injury rat model[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2019.01.003.

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GPX-1在大鼠肝缺血再灌注损伤模型的表达变化

Expression change of GPX1 in hepatic ischemiareperfusion injury rat model

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