无创性延迟肢体缺血预适应对大鼠脑缺血再灌注损伤的保护作用

doi:10.3969/j.issn.10073205.2017.11.002

河北医科大学学报

无创性延迟肢体缺血预适应对大鼠脑缺血再灌注损伤的保护作用

1.Department of Geriatrics and Gerontology, the First Hospital of Shijiazhuang City, Hebei Province，
Shijiazhuang 050011, China; 2.Department of Traditional Chinese Medicine, the First Hospital of
Shijiazhuang City, Hebei Province， Shijiazhuang 050011, China

出版日期:2017-11-25 发布日期:2017-11-16
作者简介:赵会颖（1964-），女，天津人，河北省石家庄市第一医院主任医师，医学硕士，从事老年病诊治研究。
基金资助:
河北省科技计划项目（15277785D）

The protective effect of noninvasive delayed limb ischemic preconditioning on cerebral ischemia reperfusion injury in rats#br#

1.河北省石家庄市第一医院老年病科，河北石家庄 050011；2.河北省石家庄市第一医院中医科，河北石家庄 050011

Online:2017-11-25 Published:2017-11-16

摘要/Abstract

摘要： 观察无创性肢体缺血预适应(noninvasive delayed limbischemic preconditioning，NDLIP)对大鼠脑缺血再灌注损伤的保护作用。
〖HTH〗方法〖HTSS〗〖KG*2〗将30只健康雄性SD大鼠随机分成假手术组、缺血再灌注组和NDLIP组各10只。NDLIP组大鼠给予左侧后肢缺血5 min、再灌注5 min，每天1次，连续操作3 d，然后再制作缺血模型；假手术组大鼠给予同样的麻醉和操作，但不进行颈总动脉的闭塞；假手术组和缺血再灌注组不进行NDLIP操作。检测各组大鼠海马超氧化物歧化酶(superoxide dismutase，SOD)、丙二醛(malondialdehyde,MDA)含量和Bax、Bcl2、Caspase3蛋白表达水平。
〖HTH〗结果〖HTSS〗〖KG*2〗缺血再灌注组和NDLIP组大鼠海马MDA含量明显高于假手术组，SOD含量明显低于假手术组，Bax、Caspase3表达水平明显高于假手术组，Bcl2表达水平明显低于假手术组；NDLIP组大鼠海马MDA含量明显低于缺血再灌注组，SOD含量明显高于缺血再灌注组，Bax、Caspase3表达水平明显低于缺血再灌注组，Bcl2 的表达水平明显高于缺血再灌注组。
〖HTH〗结论〖HTSS〗〖KG*2〗NDLIP能显著减少脑缺血再灌注损伤后凋亡的发生和氧化应激损伤，对脑缺血再灌注损伤具有保护作用。

关键词: 脑缺血, 再灌注损伤, 肢体远端缺血预适应, 大鼠

Abstract: To study the protective effect of noninvasive delayed limb ischemia preconditioning（NDLIP） on cerebral ischemia reperfusion injury in rats.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirty healthy male SD rats randomly divided into 3 groups, Shamoperated group, ischemicreperfusion group and NDLIP group(n=10/group). Rats in NDLIP group were subjected to 5 minutes ischemia/5 minutes reperfusion on the left hind limb once a day for 3 consecutive days, then all rats were subjected to cerebral ischemia reperfusion injury. The Shamoperated group gave the same anesthesia and operation, but did not carry out the occlusion of the common carotid artery. The shamoperated group and ischemicreperfusion group were not performed NDLIP operation. The expression of superoxide dismutase(SOD) and malondialdehyde(MDA) was detected by the kit. The protein expression of Bax, Bcl2 and Caspase3 were detected by Western blotting.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the rats in the Shamoperation group, the contents of the hippocampal MDA, Bax and Casaspe3 were significantly higher in the ischemicreperfusion group. Compared with the rats in the ischemicreperfusion group, the contents of the hippocampal MDA, Bax and Casaspe3 were significantly decreased in the NDLIP group. Compared with the rats in the Shamoperation group, the contents of the hippocampal SOD and Bcl2 were significantly decreased in the ischemicreperfusion group. Compared with the rats in the ischemicreperfusion group, the contents of the hippocampal SOD and Bcl2 were significantly increased in the NDLIP group.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓NDLIP could significantly reduce the occurrence of apoptosis and oxidative stress damage after cerebral ischemia reperfusion injury, it has protective effect on cerebral ischemia reperfusion injury.

Key words: brain ischemia, reperfusion injury； limb ischemia preconditioning, rats

赵会颖1，杨永刚1，张建平1，李贵琴1，钱玉中2，李倩倩1. 无创性延迟肢体缺血预适应对大鼠脑缺血再灌注损伤的保护作用[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2017.11.002.

ZHAO Huiying1, YANG Yonggang1, ZHANG Jianping1, LI Guiqin1, QIAN Yuzhong2, LI Qianqian1. The protective effect of noninvasive delayed limb ischemic preconditioning on cerebral ischemia reperfusion injury in rats#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2017.11.002.

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无创性延迟肢体缺血预适应对大鼠脑缺血再灌注损伤的保护作用

The protective effect of noninvasive delayed limb ischemic preconditioning on cerebral ischemia reperfusion injury in rats#br#

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